Abstract: Compositions and methods for the transcription of polynucleotide sequences encoding polypeptides in cells are provided. The cells and methods provided are useful for controlling expression of polypeptides for a wide variety of purposes.

Abstract: Power generating devices and systems that include devices, apparatus, systems and methods with a turbine assembly disposed in a recess under the road surface as the sustainable actuating mechanism include a pivoting treadle plate upon which is mounted a counterweight wherein the force of gravity raises load-bearing pivoting treadle plate that spans a recess below the roadway to a slope relative to roadways and also utilizes the movement of wheels of automobiles driving over the upper surface of the angled pivoting treadle plates or flaps with hinges to drive down the treadle plates to be substantially level and flush with the roadway as kinetic power to push down a downshaft, Pitman arm and crank to convert linear force to rotational energy to spin electric generators to generate electricity.

Abstract: The present inventions provide mammalian cells that comprise multiple Stable Integration Sites. The inventions provide sites introduced genomically into a Genomic Safe Harbor and introduced genomically outside of that particular Genomic Safe Harbor, including but not limited to another Genomic Safe Harbor. Polynucleotides of interest that encode polypeptides or RNAs of interest can be inserted into the Stable Integration Sites provided according to the inventions. The cells and methods of the inventions can be used for the high yield production of any protein, including viral proteins. Additionally, the cells and methods of the inventions are useful for production of viral vectors, such as AAV, antibodies and other proteins.

Abstract: The present inventions provide eukaryotic cells, such as mammalian cells, that comprise adeno-associated virus (AAV) polynucleotides, including AAV capsid proteins (Cap), and are capable of expressing the polypeptides encoded by the AAV polynucleotides, and thereby are capable of producing AAV, including recombinant AAV. The eukaryotic cells also may comprise adenovirus (Ad) polynucleotides. The present inventions also provide methods of expressing AAV polynucleotides, as well as Ad polynucleotides, in eukaryotic cells, such as CHO cells, HEK 293 and BHK cells. The present inventions further provides other products and methods described herein.

Abstract: An expandable and retractable wheel assembly in accordance with one or more embodiments includes a hub structure having a circular periphery and a rim expandable in circumference from a retracted circular position to one or more expanded circular positions. The rim engages and is supported by the circular periphery of the hub structure when in the retracted position. A plurality of linear actuators is attached to and supported by the hub structure. Each linear actuator includes a piston rod and a drive system for moving the piston rod from a retracted position to one or more extended positions, wherein a distal end of the piston rod engages and moves the rim when the piston rod is extended and also supports the rim in the one or more expanded rim positions.

Abstract: A drive assembly is provided and includes a rotatable housing, a motor disposed within and to rotate with the housing, the motor including a drive element and first and second drive shafts, which are independently rotatably drivable by the drive element, a first drivable element coupled to the first drive shaft such that rotation thereof is transmitted to the first drivable element and configured to propel the housing in a first direction during first drive shaft rotation and a second drivable element coupled to the second drive shaft such that rotation thereof is transmitted to the second drivable element and configured to propel the motor in a second direction, which is transversely oriented relative to the first direction, relative to the housing during second drive shaft rotation.

Abstract: An expandable and retractable wheel assembly in accordance with one or more embodiments includes a hub structure having a circular periphery and a rim expandable in circumference from a retracted circular position to one or more expanded circular positions. The rim engages and is supported by the circular periphery of the hub structure when in the retracted position. A plurality of linear actuators is attached to and supported by the hub structure. Each linear actuator includes a piston rod and a drive system for moving the piston rod from a retracted position to one or more extended positions, wherein a distal end of the piston rod engages and moves the rim when the piston rod is extended and also supports the rim in the one or more expanded rim positions.

Abstract: A drive assembly is provided and includes a rotatable housing, a motor disposed within and to rotate with the housing, the motor including a drive element and first and second drive shafts, which are independently rotatably drivable by the drive element, a first drivable element coupled to the first drive shaft such that rotation thereof is transmitted to the first drivable element and configured to propel the housing in a first direction during first drive shaft rotation and a second drivable element coupled to the second drive shaft such that rotation thereof is transmitted to the second drivable element and configured to propel the motor in a second direction, which is transversely oriented relative to the first direction, relative to the housing during second drive shaft rotation.

Abstract: A drive assembly is provided and includes a rotatable housing, a motor disposed within and to rotate with the housing, the motor including a drive element and first and second drive shafts, which are independently rotatably drivable by the drive element, a first drivable element coupled to the first drive shaft such that rotation thereof is transmitted to the first drivable element and configured to propel the housing in a first direction during first drive shaft rotation and a second drivable element coupled to the second drive shaft such that rotation thereof is transmitted to the second drivable element and configured to propel the motor in a second direction, which is transversely oriented relative to the first direction, relative to the housing during second drive shaft rotation.

Abstract: A stackable vehicle is provided and includes a deck, pairs of controllable support elements at each of a first pair of opposed minor surfaces of the deck and comprising a first leg affixed to the deck and transversely telescopic relative to a corresponding minor surface plane, a second leg coupled to a distal end of the first leg and telescopic in parallel with the corresponding minor surface plane and a pivotable and rotatable wheel disposed at a distal end of the second leg and pairs of controllable support elements at each of a second pair of opposed minor surfaces of the deck and comprising a first leg affixed to the deck, a second leg coupled to a distal end of the first leg and telescopic in parallel with a corresponding minor surface plane and a pivotable and rotatable wheel disposed at a distal end of the second leg.

Abstract: A drive assembly is provided and includes a rotatable housing, a motor disposed within and to rotate with the housing, the motor including a drive element and first and second drive shafts, which are independently rotatably drivable by the drive element, a first drivable element coupled to the first drive shaft such that rotation thereof is transmitted to the first drivable element and configured to propel the housing in a first direction during first drive shaft rotation and a second drivable element coupled to the second drive shaft such that rotation thereof is transmitted to the second drivable element and configured to propel the motor in a second direction, which is transversely oriented relative to the first direction, relative to the housing during second drive shaft rotation.

Abstract: A stackable vehicle is provided and includes a deck, pairs of controllable support elements at each of a first pair of opposed minor surfaces of the deck and comprising a first leg affixed to the deck and transversely telescopic relative to a corresponding minor surface plane, a second leg coupled to a distal end of the first leg and telescopic in parallel with the corresponding minor surface plane and a pivotable and rotatable wheel disposed at a distal end of the second leg and pairs of controllable support elements at each of a second pair of opposed minor surfaces of the deck and comprising a first leg affixed to the deck, a second leg coupled to a distal end of the first leg and telescopic in parallel with a corresponding minor surface plane and a pivotable and rotatable wheel disposed at a distal end of the second leg.

Abstract: Compositions and methods for engineered cell lines and expressions systems are provided that allow for expression of recombinant proteins in eukaryotic cells and their ease of isolation. Cell expression systems capable of expressing a protein of interest essentially free of a bound host cell protein are also provided.

Abstract: Embodiments of the subject invention relate to systems, methods, and devices providing a reduced number of selectable inputs that enable efficient communication. One aspect of what is disclosed are embodiments of a systematic code, which systematic code enables efficient communication. Another aspect of the invention is the implementation of embodiments of a systematic code on interactive devices, thereby enabling efficient communication using such interactive devices.

Abstract: A reduced number of selectable inputs that enable efficient communication. Simplified tactile and optically machine readable code of raised embossments, particularly for unsighted and visually impaired persons is provided, in which each character is represented by a cell of indicia of an intuitive systematic code. For the alphabet, the cells are configured in ordered positions, which correspond to the order of the alphabet, and thereby, assist in tactile perception so that a person may easily identify the character by touch. Also, optical machines are enabled to easily read the characters; and a keyboard is provided for producing the same.

Abstract: Embodiments of the subject invention relate to systems, methods, and devices providing a reduced number of selectable inputs that enable efficient communication. One aspect of what is disclosed are embodiments of a systematic code, which systematic code enables efficient communication. Another aspect of the invention is the implementation of embodiments of a systematic code on interactive devices, thereby enabling efficient communication using such interactive devices.

Abstract: A reduced number of selectable inputs that enable efficient communication. Simplified tactile and optically machine readable code of raised embossments, particularly for unsighted and visually impaired persons is provided, in which each character is represented by a cell of indicia of an intuitive systematic code. For the alphabet, the cells are configured in ordered positions, which correspond to the order of the alphabet, and thereby, assist in tactile perception so that a person may easily identify the character by touch. Also, optical machines are enabled to easily read the characters; and a keyboard is provided for producing the same.

Abstract: Embodiments of the subject invention relate to systems, methods, and devices providing a reduced number of selectable inputs that enable efficient communication. One aspect of what is disclosed are embodiments of a systematic code, which systematic code enables efficient communication. Another aspect of the invention is the implementation of embodiments of a systematic code on interactive devices, thereby enabling efficient communication using such interactive devices.

Abstract: An input assembly and associated technique for dialing mobile phones or otherwise providing input to a device. The device is configured for use with one hand and includes an input assembly for providing input. The input assembly includes a plurality of input controls, each (or a subset thereof) having a neutral position, a primary position and a secondary position; the neutral, primary and secondary positions corresponding to and engaging contacts to input data. When an input control is moved from a neutral position, the input control engages with a contact corresponding to the input control and that position to which the input control has been moved is detected by the device. In response to detecting the primary position, a primary datum corresponding with the input control is entered. In response to detecting the secondary position, a secondary datum corresponding with the input control is entered.

Abstract: Embodiments of the subject invention relate to systems, methods, and devices providing a reduced number of selectable inputs that enable efficient communication. One aspect of what is disclosed are embodiments of a systematic code, which systematic code enables efficient communication. Another aspect of the invention is the implementation of embodiments of a systematic code on interactive devices, thereby enabling efficient communication using such interactive devices.