Abstract: The present invention provides a Mitrecin A polypeptide useful in prevention and treatment of one or more bacteria. Also provided is a method to kill or prevent growth of one or more bacteria comprising contacting the one or more bacteria with a Mitrecin A polypeptide. The target bacteria can be selected from the group consisting of a Gram-positive bacterium, a Gram-negative bacterium, or both. In one embodiment, the present invention is drawn to a polynucleotide encoding a Mitrecin A polypeptide, a vector comprising the polynucleotide, a host cell comprising the polynucleotide, or a composition comprising the Mitrecin A polypeptide, the polynucleotide, the vector, or the host cell.

Abstract: CRISPR/Cas-related compositions and methods which provide for efficient gene editing of eukaryotic cells using modified gRNAs.

Abstract: A quaternized alkoxylated polyethylene amine can be used in a variety of industries, including the oil and gas servicing industry, as a laundry detergent, the personal care industry, as an industrial cleaner, paint, or coating, and mining operations industry. A treatment fluid comprises: a base fluid; and the surfactant. A method of treating a subterranean formation comprises introducing the treatment fluid into a well, wherein the well penetrates the subterranean formation.

Abstract: Various embodiments directed to a work stand for holding a microscopic collection device for collecting a microscopic sample from a target surface, are provided. The work stand includes a first slit formed on a top surface of the work stand and capable of holding a first microscopic collection device comprising a first holding element and a first collection platform, wherein the first collection platform of the first microscopic collection device is perpendicular to the top surface. A second slit formed on the top surface of the work stand and capable of holding a second microscopic collection device comprising a second collection platform and a second holding element at an angle to the second collection platform perpendicular to the top surface of the work stand.

Abstract: The present invention provides a Mitrecin A polypeptide useful in prevention and treatment of one or more bacteria. Also provided is a method to kill or prevent growth of one or more bacteria comprising contacting the one or more bacteria with a Mitrecin A polypeptide. The target bacteria can be selected from the group consisting of a Gram-positive bacterium, a Gram-negative bacterium, or both. In one embodiment, the present invention is drawn to a polynucleotide encoding a Mitrecin A polypeptide, a vector comprising the polynucleotide, a host cell comprising the polynucleotide, or a composition comprising the Mitrecin A polypeptide, the polynucleotide, the vector, or the host cell.

Abstract: The present invention provides a Mitrecin A polypeptide useful in prevention and treatment of one or more bacteria. Also provided is a method to kill or prevent growth of one or more bacteria comprising contacting the one or more bacteria with a Mitrecin A polypeptide. The target bacteria can be selected from the group consisting of a Gram-positive bacterium, a Gram-negative bacterium, or both. In one embodiment, the present invention is drawn to a polynucleotide encoding a Mitrecin A polypeptide, a vector comprising the polynucleotide, a host cell comprising the polynucleotide, or a composition comprising the Mitrecin A polypeptide, the polynucleotide, the vector, or the host cell.

Abstract: An additive comprising: a polymeric weak acid, wherein the polymeric weak acid is in a free acid form or in a monovalent, divalent, or trivalent salt form of the polymeric weak acid, and wherein the additive converts an invert emulsion having an external phase comprising a hydrocarbon liquid to an emulsion having an external phase comprising water. The additive can be added to the invert emulsion in a dry form or included in a base fluid. The invert emulsion can include polyvalent cations. The base fluid can also include a second additive comprising polyvalent cations.

Abstract: Embodiments disclosed herein include needle-free syringe and cap assemblies and filling tub systems. One embodiment of filling tub system includes a filling tub having exterior walls and a floor. The floor is formed into a plurality of sockets configured to receive and support a plurality of cap and syringe body assemblies, with each cap and syringe assembly received in a separate socket. Furthermore, each socket is configured to receive and support a syringe and cap assembly with contact only between one or more surfaces of the socket and selected outer surfaces of the cap. Methods of pre-filling a quantity of needle-free syringes are also disclosed.

Abstract: Techniques are provided for locating and validating single-nucleotide polymorphism (SNP) islands by scanning a reference genome. A system scans a reference genome to locate a high-variance region containing at least a minimum number of known impactful SNP locations in less than a maximum length, wherein the high-variance region is flanked by low-variance regions of at least minimum length and each having fewer than a maximum number of known relevant SNP locations. The system allows for tuning maximum and minimum region lengths, maximum and minimum SNP-location quantities, and rate-of-occurrence thresholds that define relevant and/or impactful SNP locations. Primers are designed for portions of the low-variance regions that are unique within the reference genome, wherein the primers amplify for the high-variance region.

Abstract: The present invention provides a Mitrecin A polypeptide useful in prevention and treatment of one or more bacteria. Also provided is a method to kill or prevent growth of one or more bacteria comprising contacting the one or more bacteria with a Mitrecin A polypeptide. The target bacteria can be selected from the group consisting of a Gram-positive bacterium, a Gram-negative bacterium, or both. In one embodiment, the present invention is drawn to a polynucleotide encoding a Mitrecin A polypeptide, a vector comprising the polynucleotide, a host cell comprising the polynucleotide, or a composition comprising the Mitrecin A polypeptide, the polynucleotide, the vector, or the host cell.

Abstract: A quaternized alkoxylated polyethylene amine can be used in a variety of industries, including the oil and gas servicing industry, as a laundry detergent, the personal care industry, as an industrial cleaner, paint, or coating, and mining operations industry. A treatment fluid comprises: a base fluid; and the surfactant. A method of treating a subterranean formation comprises introducing the treatment fluid into a well, wherein the well penetrates the subterranean formation.

Abstract: Various embodiments directed to a work stand for holding a microscopic collection device for collecting a microscopic sample from a target surface, are provided. The work stand includes a first slit formed on a top surface of the work stand and capable of holding a first microscopic collection device comprising a first holding element and a first collection platform, wherein the first collection platform of the first microscopic collection device is perpendicular to the top surface. A second slit formed on the top surface of the work stand and capable of holding a second microscopic collection device comprising a second collection platform and a second holding element at an angle to the second collection platform perpendicular to the top surface of the work stand.

Abstract: The present invention provides a Mitrecin A polypeptide useful in prevention and treatment of one or more bacteria. Also provided is a method to kill or prevent growth of one or more bacteria comprising contacting the one or more bacteria with a Mitrecin A polypeptide. The target bacteria can be selected from the group consisting of a Gram-positive bacterium, a Gram-negative bacterium, or both. In one embodiment, the present invention is drawn to a polynucleotide encoding a Mitrecin A polypeptide, a vector comprising the polynucleotide, a host cell comprising the polynucleotide, or a composition comprising the Mitrecin A polypeptide, the polynucleotide, the vector, or the host cell.

Abstract: A microscopic collection device, method for collecting a microscopic sample from a target surface, and the work stand for holding the microscopic collection device are provided. The microscopic collection device includes a holding element comprising a handle at one end and a collection platform connected to the holding element at a second end. The collection platform includes a collection surface on which the microscopic sample is deposited when the microscopic collection device comes into contact with the target surface. A tape is inserted through a slit and wrapped around the collection platform. The work stand includes a slit capable of holding the microscopic collection device such that the collection platform is perpendicular to the top surface of the work stand. A microscopic sample, collected using the microscopic collection device, is not only collected from the target surface but is also concentrated onto the collection face of the tape.

Abstract: Embodiments disclosed herein include needle-free syringe and cap assemblies and filling tub systems. One embodiment of filling tub system includes a filling tub having exterior walls and a floor. The floor is formed into a plurality of sockets configured to receive and support a plurality of cap and syringe body assemblies, with each cap and syringe assembly received in a separate socket. Furthermore, each socket is configured to receive and support a syringe and cap assembly with contact only between one or more surfaces of the socket and selected outer surfaces of the cap. Methods of pre-filling a quantity of needle-free syringes are also disclosed.

Abstract: The present invention provides a Mitrecin A polypeptide useful in prevention and treatment of one or more bacteria. Also provided is a method to kill or prevent growth of one or more bacteria comprising contacting the one or more bacteria with a Mitrecin A polypeptide. The target bacteria can be selected from the group consisting of a Gram-positive bacterium, a Gram-negative bacterium, or both. In one embodiment, the present invention is drawn to a polynucleotide encoding a Mitrecin A polypeptide, a vector comprising the polynucleotide, a host cell comprising the polynucleotide, or a composition comprising the Mitrecin A polypeptide, the polynucleotide, the vector, or the host cell.

Abstract: Embodiments disclosed herein include needle-free syringe and cap assemblies and filling tub systems. One embodiment of filling tub system includes a filling tub having exterior walls and a floor. The floor is formed into a plurality of sockets configured to receive and support a plurality of cap and syringe body assemblies, with each cap and syringe assembly received in a separate socket. Furthermore, each socket is configured to receive and support a syringe and cap assembly with contact only between one or more surfaces of the socket and selected outer surfaces of the cap. Methods of pre-filling a quantity of needle-free syringes are also disclosed.

Abstract: The present invention provides a Mitrecin A polypeptide useful in prevention and treatment of one or more bacteria. Also provided is a method to kill or prevent growth of one or more bacteria comprising contacting the one or more bacteria with a Mitrecin A polypeptide. The target bacteria can be selected from the group consisting of a Gram-positive bacterium, a Gram-negative bacterium, or both. In one embodiment, the present invention is drawn to a polynucleotide encoding a Mitrecin A polypeptide, a vector comprising the polynucleotide, a host cell comprising the polynucleotide, or a composition comprising the Mitrecin A polypeptide, the polynucleotide, the vector, or the host cell.

Abstract: CRISPR/Cas-related compositions and methods which provide for efficient gene editing of eukaryotic cells using modified gRNAs.