Patents by Inventor Michael J. Knauf

Michael J. Knauf has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Interleukin-2 muteins and polymer conjugation thereof
    Patent number: 5206344
    Abstract: Muteins of IL-2 in which one of the amino acids of the mature native sequence of IL-2 is replaced by a cysteine residue are prepared and conjugated through the replaced cysteine residue to a polymer selected from polyethylene glycol homopolymers or polyoxyethylated polyols, wherein said homopolymers are unsubstituted or substituted at one end with an alkyl group. These muteins are made via host expression of mutant genes encoding the muteins that have been changed from the genes for the parent proteins by site-directed mutagenesis. In addition, other species of IL-2 may be conjugated via the cysteine residue at position 125 of the mature IL-2 protein that is not necessary for the biological activity of the IL-2.
    Type: Grant
    Filed: January 11, 1988
    Date of Patent: April 27, 1993
    Assignee: Cetus Oncology Corporation
    Inventors: Nandini Katre, Robert F. Halenbeck, Robert J. Goodson, Peter C. McCabe, Michael J. Knauf
  • Succinylated interleukin-2 for pharmaceutical compositions
    Patent number: 4931544
    Abstract: A pharmaceutical composition is prepared wherein biologically active conjugated interleukin-2 is dissolved in an aqueous carrier medium without the presence of a solubilizing agent. The unconjugated IL-2, which is not water soluble or not readily soluble in water at pH 6-8 without such solubilizing agent, is selectively conjugated to one or more succinyl groups by reaction with succinic anhydride.
    Type: Grant
    Filed: January 27, 1988
    Date of Patent: June 5, 1990
    Assignee: Cetus Corporation
    Inventors: Nandini Katre, Michael J. Knauf
  • Solubilization of immunotoxins for pharmaceutical compositions using polymer conjugation
    Patent number: 4917888
    Abstract: A pharmaceutical composition is prepared wherein a biologically active conjugated protein which is .beta.-interferon, interleukin-2, or an immunotoxin is dissolved in an aqueous carrier medium without the presence of a solubilizing agent. The unconjugated protein, which is not water-soluble or not readily soluble in water at pH 6-8 without such solubilizing agent, is selectively conjugated to a water-soluble polymer selected from polyethylene glycol homopolymers or polyoxyethylated polyols.
    Type: Grant
    Filed: December 11, 1987
    Date of Patent: April 17, 1990
    Assignee: Cetus Corporation
    Inventors: Nandini Katre, Michael J. Knauf
  • Solubilization of proteins for pharmaceutical compositions using polymer conjugation
    Patent number: 4766106
    Abstract: A pharmaceutical composition is prepared wherein a biologically active conjugated protein which is .beta.-interferon, interleukin-2, or an immunotoxin is dissolved in an aqueous carrier medium without the presence of a solubilizing agent. The unconjugated protein, which is not water-soluble or not readily soluble in water at pH 6-8 without such solubilizing agent, is selectively conjugated to a water-soluble polymer selected from polyethylene glycol homopolymers or polyoxyethylated polyols.
    Type: Grant
    Filed: January 25, 1988
    Date of Patent: August 23, 1988
    Assignee: Cetus Corporation
    Inventors: Nandini Katre, Michael J. Knauf