Abstract: Disclosed is an RNA allotopic strategy to complement and genetically rescue mitochondrial gene defects. This approach can permit rescue of a mitochondrial DNA mutant by allotopic expression of a full-length recoded mitochondrial RNA that is transcribed in the nucleus, successfully imported into the mitochondria, and expressed there.

Abstract: Described herein is a mitochondrial-targeted RNA expression system (mtTRES) for delivery of RNA molecules to mitochondria. mtTRES vectors generate RNAs in vivo that are un-capped, non-polyadenylated, and actively directed to mitochondria. The disclosed vectors are capable of delivering either non-coding RNA molecules or RNA molecules encoding a protein of interest to the mitochondria. In particular, the disclosed vectors include (1) an RNAPIII initiation (promoter) sequence, (2) a non-coding leader sequence (NCL), (3) a mitochondrial translation initiation sequence and an ORF encoding a protein of interest, or a sequence encoding a non-coding RNA, and (4) an RNAPIII termination sequence.

Abstract: Described herein is a mitochondrial-targeted RNA expression system (mtTRES) for delivery of RNA molecules to mitochondria. mtTRES vectors generate RNAs in vivo that are un-capped, non-polyadenylated, and actively directed to mitochondria. The disclosed vectors are capable of delivering either non-coding RNA molecules or RNA molecules encoding a protein of interest to the mitochondria. In particular, the disclosed vectors include (1) an RNAPIII initiation (promoter) sequence, (2) a non-coding leader sequence (NCL), (3) a mitochondrial translation initiation sequence and an ORF encoding a protein of interest, or a sequence encoding a non-coding RNA, and (4) an RNAPIII termination sequence.

Abstract: A portable table system detachably secured upon various supporting members, such as vertical or angled columns or trees and horizontal rails. The system includes a tabletop having a first and second section each with a cutout for receiving the vertical or angled support member, first and second hook members extending from the underside of the tabletop, and a clamping assembly for being attached to the support member and being coupled to the tabletop via the hook members. Latching assemblies are used to attach the first and second sections together. Fillers may be used to partially fill the cutouts such as when the support member is relatively small or to completely fill the cutouts such as when attached to a horizontal support member. The clamping assembly may include a structure suitable for receiving flat-sided, rounded, or irregular-shaped support members. An optional legged-base assembly may also be used as the support member.

Abstract: A method for obtaining a Drosophila neurodegeneration mutant includes the steps of selecting at least one Drosophila mutant having an aberrant phenotype selected from the group consisting of temperature-sensitive paralysis and bang-sensitive paralysis; and screening the at least one selected Drosophila mutant for age-dependent neurodegeneration. Neurodegeneration mutants are obtained in the method and causal mutations are characterized. The neurodegeneration mutants can be used in screening methods to identify putative neuroprotective agents.

Abstract: A method for obtaining a Drosophila neurodegeneration mutant includes the steps of selecting at least one Drosophila mutant having an aberrant phenotype selected from the group consisting of temperature-sensitive paralysis and bang-sensitive paralysis; and screening the at least one selected Drosophila mutant for age-dependent neurodegeneration. Neurodegeneration mutants are obtained in the method and causal mutations are characterized. The neurodegeneration mutants can be used in screening methods to identify putative neuroprotective agents.

Abstract: A refrigerated cabinet with adjustable shelves includes a cabinet having a molded plastic liner defining a refrigeration compartment. A plurality of shelves are disposed in the refrigeration compartment. Shelf supports are integrally molded with the liner to support said plurality of shelves in both horizontal and tilted positions. Integrally formed back stops define channels for air to flow vertically through the cabinet and help lock the shelves in place to prevent accidental dislodging. The liner also defines a housing for the evaporator coil and fan in the upper portion of the liner. Shoulders are integrally formed along the top corners of the liner. The fan panel mounts to the shoulders on opposing sides of the liner and serves as the bottom of the evaporator housing.

Abstract: A refrigeration system includes a base having an inlet opening, an outlet opening, an evaporator pan, a condenser pan, and a compressor mounting surface all integrally formed therein. All of the components of the refrigeration system mount onto the base to from a unitary structure. An evaporator is mounted to the base above said evaporator pan. A condenser is mounted to said base above said condenser pan. A compressor mounted to the compressor mounting surface and operatively connected to said evaporator and said condenser. A cover encloses the inlet opening, outlet opening and evaporator.

Abstract: A refrigeration system includes a base having an inlet opening, an outlet opening, an evaporator pan, a condenser pan, and a compressor mounting surface all integrally formed therein. All of the components of the refrigeration system mount onto the base to from a unitary structure. An evaporator is mounted to the base above said evaporator pan. A condenser is mounted to said base above said condenser pan. A compressor mounted to the compressor mounting surface and operatively connected to said evaporator and said condenser. A cover encloses the inlet opening, outlet opening and evaporator. The cover and base include an integral locking mechanism to secure the cover to the base.

Abstract: A device for forming a solid support surface adjacent an open wire shelf having a front support member, a rear support member and a plurality of intermediate wire members which have longitudinal openings therebetween wherein the solid support surface is flexibly resilient to be snapped in place between a slot defined in the ticket molding secured along the front support member of the open wire shelf and the rear support member thereof. The cover plate of the solid shelf device preferably is of a plastic or spring steel material to facilitate placement thereof between a slot defined by the ticket molding and the rear support member.