Abstract: A system and method for bio-analysis of complex samples which enables processing, biomolecule capture, and/or immunoassay detection. The system, capable of operating with a low hydrodynamic force, has at least one capillary, at least one microwell, a filtration well, and a reagent well. The system is able to process whole blood, serum, plasma, urine, wound fluid, bronchial lavage, and sputum specimens, along with any other complex biological samples with volumes from 0.1 ?L to 100 mL. Biomarkers can be whole cells or cell-free biomarkers.

Abstract: A method of using electrochemical reporters in a micro-filtration sensor which contains multiple analyte detection wells with electrodes and where the electrochemical reporters determine the identity and integrity of the product and sample. Identity and integrity are assessed for suitable use and linkage to additional data or preventing producing erroneous in-vitro diagnostic data. The micro-filtration sensor is additionally used for electrochemical bioassays of analytes in one or more of the analyte detection microwells. Analyte detection microwells may contain an additional affinity agent for capture of affinity agents for analyte detection and electrochemical reporters for identity and integrity detection.

Abstract: A microwell microelectrode filtration sensor created by using an inert planar surface, which can be a metal pattern dry etched, to generate a filtration membrane with pores, generating a reactive metal surface layer as a working electrode. The electrode is within an area covering a filtration membrane, and makes one or more microwells added by a first layer of inert plastic of an effective working distance height and a second layer of a non-reactive metal of an effective microwell surface area to serve counter and/or reference an electrode. This is followed by at least one layer of inert plastic or metal.

Abstract: A method for the analysis of one or more non-invasive sample biomarkers. Biomarkers within a normal concentration range may indicate not only the lack of disease, but also general health or lack of disease. These non-invasive sample biomarkers are corrected based on excretion of another non-invasive sample biomarker reflecting changes to the sample volume in a normal concentration range lacking disease. Method allows for indicating the existence of diseases, such as renal insufficiency, infections or other disease biomarkers by analyzing the concentration of biomarkers.

Abstract: An electrochemical analyte detection method of cells and biomolecules that does not interfere with optical, genetic, or mass spectrometric detection allows reagents to be added simultaneously. Additional reagents can be included as mass reporters for identification of an analyte and measuring analyte integrity. Analyte integrity and identity allow electronic coupling of electrochemical analyte results to additional optical, genetic, or mass spectrometric results for the analyte. Reagents for mass reporters, electrochemical, and mass spectrometric detection can be added simultaneously to analyte detection microwells with size exclusion filters used for electrochemical signal generation and affinity agents for capturing mass reporters and analytes.

Abstract: A method and system for cellular response analysis whereby cells are captured into an analyte detection microwell with a size exclusion filter using an affinity agent to capture an analyte, such as a specific cell or biomolecule, by a cleavable linkage arm connected to the size exclusion filter where the affinity captured analyte, such as a cell or biomolecule, does not pass through the size exclusion filter at the bottom of the microwell, but analyte, not affinity captured, do pass along with debris from the sample.

Abstract: A method for collection of complex samples and bio-analysis of the same. Specifically, a system having a porous wicking matrix, at least one capillary, analyte detection microwell was with porous surface and a filtration well, for bio-analysis of complex samples, which enable processing of biomolecule capture and/or immunoassay detection. The system allows for processing samples such as: wholeblood, serum, plasma, urine, wound fluid, bronchial lavage, and sputum. Amounts available for measure range from 0.1 ?L to 1 mL.

Abstract: Collection of target analytes from complex samples is performed in detection microwell which includes a size exclusion filter and allows incubating the target analyte with affinity agents for a target analyte for capture and removal of non-target molecules. Detection of the target analytes by collection of the complexes in close proximity to a working electrode and a reference electrode facilitates the detection electrochemical labels produced.

Abstract: The timing of the reaction of moisture-sensitive reagents for detecting the presence of analytes, e.g. leukocytes in urine samples, is used to detect when the reagents have been compromised by excess humidity. The ratio of light reflectance at wavelengths characteristic of the products of reaction between the reagents and the analyte and an infra-red reference dye is measured at two preset times after a urine sample has been applied to a test strip and used to determine whether the reagents have been compromised by excessive humidity. The presence of unusually dark samples is determined from the reflected light at 470 and 625 nm in order to confirm that the test strips are humidity-compromised.

Abstract: The timing of the reaction of moisture-sensitive reagents for detecting the presence of analytes, e.g. leukocytes in urine samples, is used to detect when the reagents have been compromised by excess humidity. The ratio of light reflectance at wavelengths characteristic of the products of reaction between the reagents and the analyte and an infra-red reference dye is measured at two preset times after a urine sample has been applied to a test strip and used to determine whether the reagents have been compromised by excessive humidity. The presence of unusually dark samples is determined from the reflected light at 470 and 625 nm in order to confirm that the test strips are humidity-compromised.

Abstract: The timing of the reaction of moisture-sensitive reagents for detecting the presence of analytes, e.g. leukocytes in urine samples, is used to detect when the reagents have been compromised by excess humidity. The ratio of light reflectance at wavelengths characteristic of the products of reaction between the reagents and the analyte and an infra-red reference dye is measured at two preset times after a urine sample has been applied to a test strip and used to determine whether the reagents have been compromised by excessive humidity. The presence of unusually dark samples is determined from the reflected light at 470 and 625 nm in order to confirm that the test strips are humidity-compromised.

Abstract: Methods are disclosed for determining progression of a condition, onset of a condition, or efficacy of treatment of a condition characterized by an adipocyte imbalance in a patient. In addition, disclosed herein are methods of treating diabetes, abnormal adipocyte activity, and insulin resistance using monomeric, homodimeric, and heterodimeric forms of certain C-terminal fragments of adiponectin receptor. In addition, methods of treating abnormal adipocyte activity, treating metabolic syndrome, causing insulin secretion, increasing insulin levels, inhibiting insulin degradation enzyme, treating Alzheimer's disease, treating cardiovascular disease associated with adiponectin levels, inhibiting ADAM-17 enzyme, inhibiting a protease, treating a condition associated with TNF-alpha, and treating a condition associated with HER2-neu are disclosed. Compositions, dosage forms, and kits are also disclosed.

Abstract: The timing of the reaction of moisture-sensitive reagents for detecting the presence of analytes, e.g. leukocytes in urine samples, is used to detect when the reagents have been compromised by excess humidity. The ratio of light reflectance at wavelengths characteristic of the products of reaction between the reagents and the analyte and an infra-red reference dye is measured at two preset times after a urine sample has been applied to a test strip and used to determine whether the reagents have been compromised by excessive humidity. The presence of unusually dark samples is determined from the reflected light at 470 and 625 nm in order to confirm that the test strips are humidity-compromised.

Abstract: Sample media for the analysis of analytes in a fluid test sample includes a carrier, at least one test field on the surface of the carrier including at least one reagent reactive with the analytes and capable of providing a detectable response. A non-visible bar code formed by at least two distinct non-visible marker fields is located on the carrier. The marker fields are configured to reflect electro-magnetic (EM) radiation within one or more ranges of non-visible wavelengths to form a coded sequence of reflectances correlated to identification of the sample media.

Abstract: A substrate, which is useful for performing biological, chemical and diagnostic assays, and a method for preparing the substrate are provided. The substrate has an upper surface with a coating disposed thereon. The coating comprises a charged polymer, a non-ionic polyether, and a silicate compound. The substrate can increase capture phase binding and reduce non-specific binding of biomolecules for a biological microarray.

Abstract: The timing of the reaction of moisture-sensitive reagents for detecting the presence of analytes, e.g. leukocytes in urine samples, is used to detect when the reagents have been compromised by excess humidity. The ratio of light reflectance at wavelengths characteristic of the products of reaction between the reagents and the analyte and an infra-red reference dye is measured at two preset times after a urine sample has been applied to a test strip and used to determine whether the reagents have been compromised by excessive humidity. The presence of unusually dark samples is determined from the reflected light at 470 and 625 nm in order to confirm that the test strips are humidity-compromised.

Abstract: The timing of the reaction of moisture-sensitive reagents for detecting the presence of analytes, e.g. leukocytes in urine samples, is used to detect when the reagents have been compromised by excess humidity. The ratio of light reflectance at wavelengths characteristic of the products of reaction between the reagents and the analyte and an infra-red reference dye is measured at two preset times after a urine sample has been applied to a test strip and used to determine whether the reagents have been compromised by excessive humidity. The presence of unusually dark samples is determined from the reflected light at 470 and 625 nm in order to confirm that the test strips are humidity-compromised.

Abstract: A coating formula and method for surface coating non-porous surfaces. Microfluidic devices including said coating achieve desired properties including increased hydrophilicity, improved adhesion, stability and optical clarity.

Abstract: Methods are disclosed for determining progression of a condition, onset of a condition, or efficacy of treatment of a condition characterized by an adipocyte imbalance in a patient. In addition, disclosed herein are methods of treating diabetes, abnormal adipocyte activity, and insulin resistance using monomeric, homodimeric, and heterodimeric forms of certain C-terminal fragments of adiponectin receptor. In addition, methods of treating abnormal adipocyte activity, treating metabolic syndrome, causing insulin secretion, increasing insulin levels, inhibiting insulin degradation enzyme, treating Alzheimer's disease, treating cardiovascular disease associated with adiponectin levels, inhibiting ADAM-17 enzyme, inhibiting a protease, treating a condition associated with TNF-alpha, and treating a condition associated with HER2-neu are disclosed. Compositions, dosage forms, and kits are also disclosed.

Abstract: A substrate, which is useful for performing biological, chemical and diagnostic assays, and a method for preparing the substrate are provided. The substrate has an upper surface with a coating disposed thereon. The coating comprises a charged polymer, a non-ionic polyether, and a silicate compound. The substrate can increase capture phase binding and reduce non-specific binding of biomolecules for a biological microarray.