Abstract: Methods and compositions are provided for producing full-length cDNA libraries. In the subject methods, full length first strand cDNAs are isolated using a fusion protein of an eIF-4E domain and an eIF-4G domain separated by a flexible linker. Also provided is the novel fusion protein employed in the subject methods, as well as nucleic acids encoding, and host cells capable of expressing, the same. Finally, kits for use in practicing the subject methods are provided. The subject invention finds use in a variety of applications in which full-length cDNA libraries are employed.

Abstract: Methods and compositions are provided for producing full-length cDNA libraries. In the subject methods, full length first strand cDNAs are isolated using a fusion protein of an eIF-4E domain and an eIF-4G domain separated by a flexible linker. Also provided is the novel fusion protein employed in the subject methods, as well as nucleic acids encoding, and host cells capable of expressing, the same. Finally, kits for use in practicing the subject methods are provided. The subject invention finds use in a variety of applications in which full-length cDNA libraries are employed.

Abstract: A rectangular sheet of extruded, twin-walled, fluted plastic fabricated from a polyethylene copolymer is scored with N parallel lines, wherein N is any odd number three (3) or greater. The sheet of plastic is then bent or otherwise configured along the N parallel lines to form N+1 panels. The first and last panels are snapped into place along the opposing sidewalls, respectively, of a shipping container. The intermediate panels are bent into N + 1 2 V-shaped configurations which together will hold the bottom portion of a load of dry particle materials which will be shipped or stored therein. The materials may be loaded and unloaded through parts.

Abstract: Methods and compositions are provided for producing full-length CDNA libraries. In the subject methods, full length first strand cDNAs are isolated using a fusion protein of an eIF-4E domain and an eIF-4G domain separated by a flexible linker. Also provided is the novel fusion protein employed in the subject methods, as well as nucleic acids encoding, and host cells capable of expressing, the same. Finally, kits for use in practicing the subject methods are provided. The subject invention finds use in a variety of applications in which full-length cDNA libraries are employed.

Abstract: Methods and compositions are provided for producing full-length cDNA libraries. In the subject methods, full length first strand cDNAs are isolated using a fusion protein of an eIF-4E domain and an eIF-4G domain separated by a flexible linker. Also provided is the novel fusion protein employed in the subject methods, as well as nucleic acids encoding, and host cells capable of expressing, the same. Finally, kits for use in practicing the subject methods are provided. The subject invention finds use in a variety of applications in which full-length cDNA libraries are employed.

Abstract: Methods and compositions are provided for producing full-length cDNA libraries. In the subject methods, full length first strand cDNAs are isolated using a fusion protein of an eIF-4E domain and an eIF-4G domain separated by a flexible linker. Also provided is the novel fusion protein employed in the subject methods, as well as nucleic acids encoding, and host cells capable of expressing, the same. Finally, kits for use in practicing the subject methods are provided. The subject invention finds use in a variety of applications in which full-length cDNA libraries are employed.

Abstract: Methods and compositions are provided for producing full-length cDNA libraries. In the subject methods, full length first strand cDNAs are isolated using a fusion protein of an eIF-4E domain and an eIF-4G domain separated by a flexible linker. Also provided is the novel fusion protein employed in the subject methods, as well as nucleic acids encoding, and host cells capable of expressing, the same. Finally, kits for use in practicing the subject methods are provided. The subject invention finds use in a variety of applications in which full-length CDNA libraries are employed.

Abstract: Methods and compositions are provided for producing full-length cDNA libraries. In the subject methods, full length first strand cDNAs are isolated using a fusion protein of an eIF-4E domain and an eIF-4G domain separated by a flexible linker. Also provided is the novel fusion protein employed in the subject methods, as well as nucleic acids encoding, and host cells capable of expressing, the same. Finally, kits for use in practicing the subject methods are provided. The subject invention finds use in a variety of applications in which full-length cDNA libraries are employed.

Abstract: Methods are provided for preparing a double-stranded cDNA corresponding to the 5' end of an mRNA. In the subject methods, an mRNA is first contacted with an oligo dT primer under first strand cDNA synthesis conditions. Next, the resultant hybrid is contacted with a random primer under first strand cDNA synthesis conditions, such that a cDNA complementary to the 5' end of the mRNA is produced. The resultant hybrid molecule is converted to two different double-stranded cDNAs, a first cDNA comprising the oligo dT primer and a second cDNA lacking the oligo dT primer. The two double-stranded cDNAs are then separated from each other. The subject methods find use in a variety of applications, and find particular use in the synthesis of 5' enriched cDNA libraries. Also provided are cDNA libraries produced by the subject methods, as well as kits for performing the subject methods.

Abstract: The present invention provides an improved container holder having a unique shape that facilitates efficient manufacturing of the holder and printing of advertising material at a predetermined location on the holder. The container holder comprises an elongate body delimited by top and bottom edges and first and second end edges. The top and bottom edges define straight lines that are parallel to each other. The body further comprises a first tab positioned at the first end edge of the body, and a second tab positioned interiorly of the body, and generally adjacent to the first tab. The tabs are positioned at an acute angle with respect to one of the top and bottom edges, and extend away from each other in opposite directions. The body further comprises a first slot positioned adjacent the second end edge, and a second slot positioned generally adjacent the first slot.

Abstract: A standard digital display alarm clock is provided which is driven by a 1.5 volt DC power source. A two-tone alarm device, whose sound level is adjustable from 60 dB to 100 dB, is electrically wired to the clock and sounds when the clock attains a predetermined time. The power for the alarm device is supplied by either a 9 volt DC source contained within the clock or a 12 volt DC automotive cigarette lighter. The clock is provided with an intermittent alert feature which will cause the alarm to sound every four minutes for an indefinite amount of time and requires operator input to deactivate.

Abstract: Caps of the type used by baseball players but also used by other athletes and their fans have an opening at the back. A strap is attached to the headband of the cap on either side of the opening and the straps can be used to adjust the cap to fit the head size of the wearer by overlapping the ends of the straps to the necessary extent. One strap usually has spaced holes and the other spaced studs which fit into the holes. The present invention provides a panel which may carry the logo of a team, a merchandiser's advertisement or other design. The panel is secured to the overlapped straps in various ways described in detail.

Abstract: In an apparatus for generating a fine dispersion of particles in a gas, a body (47) for containing the gas has an inlet (59a, 59b) and an outlet (60) for passage of the particles. Nozzles (55) are provided to direct a plurality of jets of gas through the body (47). The body (47) is maintained at an elevated temperature by a heating element (49).