Abstract: Provided herein are direct-to-library methods, systems, and compositions.

Abstract: Provided herein are fragment length profiles of nucleic acid libraries, methods of generating fragment length profiles of nucleic acid libraries and methods of using fragment length profiles for diagnostics and/or prognostics. The application further provides methods, compositions and kits for determining the infection stage or the site of localization in a subject.

Abstract: Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples.

Abstract: Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples.

Abstract: Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples.

Abstract: Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples.

Abstract: A method of selling season tickets is disclosed in which unrelated purchasers may reserve a seat ticket for one or more selected events in a related series of events. When enough seat tickets have been reserved, a season ticket for that seat is sold, and the individual tickets reserved for each purchaser are paid for and delivered to the purchasers. Provisions for canceling previously made reservations are included. The method is effectuated on one or more computers, and the computers may be connected to a computer network, including the internet, to allow use of the system by prospective purchasers at remote locations.

Abstract: A method of selling season tickets is disclosed in which unrelated purchasers may reserve a seat ticket for one or more selected events in a related series of events. When enough seat tickets have been reserved, a season ticket for that seat is sold, and the individual tickets reserved for each purchaser are paid for and delivered to the purchasers. Provisions for canceling previously made reservations are included. The method is effectuated on one or more computers, and the computers may be connected to a computer network, including the internet, to allow use of the system by prospective purchasers at remote locations.

Abstract: A container for containing a liquid that has suspended particles used for depositing on a substrate is formed of an electrically conductive material, and has a bottom wall. An atomizer for atomizing the liquid and particles to form an aerosol is mounted at an upper end thereof. A bottom wall of the container has a piezoelectric ultrasonic transducer attached to the wall. A high voltage connection to the piezoelectric transducer is in a recess formed by a lower skirt on the container and is mounted in an insulating block in the recess. A cup shaped insulating sleeve slips over the lower portions of the container and shields the high voltage connection from the exterior.

Abstract: A container for containing a liquid that has suspended particles used for depositing on a substrate is formed of an electrically conductive material, and has a bottom wall. An atomizer for atomizing the liquid and particles to form an aerosol is mounted at an upper end thereof. A bottom wall of the container has a piezoelectric ultrasonic transducer attached to the wall. A The high voltage connection to the piezoelectric transducer is in a recess formed by a lower skirt on the container and is mounted in an insulating block in the recess. A cup shaped insulating sleeve slips over the lower portions of the container and shields the high voltage connection from the exterior.

Abstract: A whole arm orthosis with plural degrees of freedom dampens limb tremors. A linkage mechanism permits movement of the forearm in a horizontal plane, as well as tilting of the forearm out of the horizontal plane. A sensor coupled to the joints of the linkage mechanism detects the angular velocity of rotation. A brake applies resistive torque to the joints in accordance with the angular velocity of rotation.

Abstract: Hand control devices are disclosed which provide for damping of vibrations and other unintended motion with three or more degrees of freedom. In one embodiment, a four degree of freedom damped hand control is illustrated, including a chamber filled with a viscous fluid, and a transmission which couples movement of a handle in the X, Y and Z directions and rotation in a .theta. direction about one of the axes to movements of one or more drag elements in the fluid. The hand control further includes a position-sensing and a rotation-sensing assembly for sensing the position of the handle relative to a reference frame and for generating electrical signals indicative of the position and angle.

Abstract: A computer-assisted method for prescribing communication devices for non-speaking patients which is objective, and is not device-limited and functions as an effective tool for clinicians prescribing communication devices. Because the method is not device-limited, it may also be used to test the effect of design changes in device characteristics which affect the fit of the device to the user and the communication rate achieved; and enables a direct comparison to be made among devices, based on scoring the user-device pair. The method comprises the compilation of data concerning client needs and preferences as well as the client cognitive and learning characteristics; assessing the patient's motor abilities for control of devices; assessing the characteristics of a variety of communication devices; and using a computer program and specially developed formulas to integrate, compare, and assess the results so as to arrive at a communication device best suited for the client's needs.

Abstract: A hand control capable of suppressing tremors and other unwanted vibrations is disclosed which incorporates viscous damping in two degrees of freedom to mechanically filter the forces applied by the user's hand. To suppress pathological intention tremors, the damping characteristics are chosen so as to selectively reduce the amplitude of movements at or above about 3 Hertz. The hand control includes a chamber filled with a viscous fluid, a position-sensing actuator assembly and a damping element connected to the actuator and disposed within the chamber to suppress involuntary movements of the actuator. The volume of the chamber, size of the damping element and viscosity of the fluid are chosen to achieve a damping constant of about 2 to 20 lbf-sec/ft., preferably from about 5 to about 15 lbf-sec/ft. The viscous fluid is preferably a silicone oil having a viscosity of about 300,000 to about 900,000 cstokes.