Abstract: In some aspects, the present disclosure provides a nanoparticle composition comprising tRNA and an aminolipid delivery compound. The aminolipid delivery compound may be a dendrimer, dendron, or dendritic lipid, a polymer such as a polyamide or polyester, or a lipid with one or more hydrophobic components. In some embodiments, these compositions may be administered to a patient to treat a genetic disease or disorder such as cystic fibrosis, Duchene muscular dystrophy, or cancer.

Abstract: In some aspects, the present disclosure provides a nanoparticle composition comprising tRNA and an aminolipid delivery compound. The aminolipid delivery compound may be a dendrimer, dendron, or dendritic lipid, a polymer such as a polyamide or polyester, or a lipid with one or more hydrophobic components. In some embodiments, these compositions may be administered to a patient to treat a genetic disease or disorder such as cystic fibrosis, Duchene muscular dystrophy, or cancer.

Abstract: In some aspects, the present disclosure provides a nanoparticle composition comprising tRNA and an aminolipid delivery compound. The aminolipid delivery compound may be a dendrimer, dendron, or dendritic lipid, a polymer such as a polyamide or polyester, or a lipid with one or more hydrophobic components. In some embodiments, these compositions may be administered to a patient to treat a genetic disease or disorder such as cystic fibrosis, Duchene muscular dystrophy, or cancer.

Abstract: Fully dense composite accurate tooling is manufactured by casting but without the requirement of subsequent machining. The tooling so provided by the invention comprises injection mold halves for mating engagement to form a mold for injecting multiple near net shapes using either thermoset or thermoplastic materials. A known vacuum casting technique used for producing parts has been modified to make tooling according to the invention. A master pattern of the near net shape or a model of the actual core and cavity of the desired mold is used to create a silicone rubber mold (SRM). Once the SRM is completed, the 3-part material (catalyst, resin and composite) is weighed to the required volume to cast into the SRM, then placed (along with the SRM) into the vacuum casting system which reaches a level of 28 inches Hg of vacuum. The 3-part composite material is thoroughly mixed for approximately 20 minutes in about 28 inches Hg of vacuum.