Abstract: The disclosure relates to a system and method of using the system to detect and monitor afferent synaptic nerve fiber function in vitro. The disclosure also relates to a method of screening for test agents or compounds that modulate nerve function, such as test agents that modulate pain sensation in a human subject, by exposing one or a plurality of test agents to systems comprising a first and second spheroid, wherein the first spheroid comprise cells from a mammalian dorsal root ganglia and the second spheroid comprises cells from a mammalian spinal cord.

Abstract: The present invention is directed to a microelectrode array for use in microengineered physiological systems and methods of using the same.

Abstract: The present disclosure generally relates to a cell culturing system, and specifically to a three-dimensional cell culturing system for neuronal cells that promotes both structural and functional characteristics that mimic those of in vivo peripheral fibers, including cell myelination. Using a dual hydrogel construct and spheroids comprising neuronal cells, the present disclosure provides methods, devices, and systems for in vitro spatially-controlled, three-dimensional models that permit intra- and extra-cellular electro-physiological measurements and recordings. The three-dimensional hydrogel constructs allow for flexibility in incorporated cell types, geometric fabrication, and electrical manipulation, providing viable systems for culture, perturbation, and testing of biomimetic neural growth with physiologically-relevant results.

Abstract: The disclosure relates to a tissue culture device and components of a system used to grow, maintain and measure recording from cells. In some embodiments, the tissue culture device is an insert with a surface onto which cells may be plated and grown. Electrodes on or near the surface of the cells can be used to measure electrophysiological data when current is applied to the system.

Abstract: The present disclosure generally relates to a cell culturing system, and specifically to a three-dimensional cell culturing system for neuronal cells that promotes both structural and functional characteristics that mimic those of in vivo peripheral fibers, including cell myelination. Using a dual hydrogel construct and explants from neuronal cells, the present disclosure provides methods, devices, and systems for in vitro spatially-controlled, three-dimensional models that permit intra- and extra-cellular electrophysiological measurements and recordings. The three-dimensional hydrogel constructs allow for flexibility in incorporated cell types, geometric fabrication, and electrical manipulation, providing viable systems for culture, perturbation, and testing of biomimetic neural growth with physiologically-relevant results.