Abstract: A fine needle aspiration biopsy tool includes a pressurized chamber having a vacuum applied to the chamber. An actuation valve engaged by the chamber and has actuation grips on opposing sides that cooperatively allow for selective fluid engagement with an environment outside of the chamber. A port extends from the actuation valve and selectively receives a needle or a cap. In operation, the actuation valve is actuated by pressing engagement of the actuator grips to apply vacuum to the needle from the vacuum chamber to draw in fluid or mass in flow communication with an inflow end of the needle during a biopsy procedure.