Patents by Inventor Michael John Tarry

Michael John Tarry has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Protein constructs of moloney murine leukemia virus reverse transcriptase (MMLV-RT)
    Patent number: 12655401
    Abstract: Described are protein constructs of Moloney murine leukemia virus reverse transcriptase (MMLV-RT). In one embodiment the protein construct comprises a Moloney murine leukemia virus reverse transcriptase (MMLV) mature protein sequence; and an expression enhancement sequence at the N-terminus of said MMLV mature protein sequence. The expression enhancement sequence is devised for providing heterologous expression of the protein construct in bacterial cells in absence of chaperone co-expression and/or to provide high-level of expression of the MMLV. In another embodiment the protein construct further comprises a tag/linker combo at the N-terminus of the expression enhancement sequence for assisting in purification of the protein construct. Also described are isolated nucleic acid molecules, vectors and methods for expressing the protein construct in cells. Advantageously these allow large-scale expression of MMLV-RT from cultures of E. coli cells and simplify protocols for purification of MMLV-RT at high purity.
    Type: Grant
    Filed: July 7, 2021
    Date of Patent: June 16, 2026
    Assignee: INTEGREON GLOBAL, INC.
    Inventors: Thomas Martin Thorne Schmeing, Michael John Tarry
  • PROTEIN CONSTRUCTS OF MOLONEY MURINE LEUKEMIA VIRUS REVERSE TRANSCRIPTASE (MMLV-RT)
    Publication number: 20230348868
    Abstract: Described are protein constructs of Moloney murine leukemia virus reverse transcriptase (MMLV-RT). In one embodiment the protein construct comprises a Moloney murine leukemia virus reverse transcriptase (MMLV) mature protein sequence; and an expression enhancement sequence at the N-terminus of said MMLV mature protein sequence. The expression enhancement sequence is devised for providing heterologous expression of the protein construct in bacterial cells in absence of chaperone co-expression and/or to provide high-level of expression of the MMLV. In another embodiment the protein construct further comprises a tag/linker combo at the N-terminus of the expression enhancement sequence for assisting in purification of the protein construct. Also described are isolated nucleic acid molecules, vectors and methods for expressing the protein construct in cells. Advantageously these allow large-scale expression of MMLV-RT from cultures of E. coli cells and simplify protocols for purification of MMLV-RT at high purity.
    Type: Application
    Filed: July 7, 2021
    Publication date: November 2, 2023
    Inventors: THOMAS MARTIN SCHMEING, Michael John Tarry