Patents by Inventor Michael Josephus Theresia Van Eijk
Michael Josephus Theresia Van Eijk has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11873529Abstract: The invention relates to a kit of parts for detecting one or more polymorphisms in a plurality of target nucleotide sequences in a plurality of samples, wherein the kit of parts comprises (i) a first probe and a second probe, wherein the first probe comprises a first target specific section and a first tag section that is non-complementary to the target nucleotide sequence and that comprises a first universal primer binding site, wherein the second probe comprises a second target specific section and a second tag section that is non-complementary to the target nucleotide sequence; and (ii) a first primer and optionally a second primer wherein the first primer comprises a sample-specific identifier sequence and wherein the first primer can hybridize to the first universal primer binding site.Type: GrantFiled: March 22, 2021Date of Patent: January 16, 2024Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Rene Cornelis Josephus Hogers
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Patent number: 11649494Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.Type: GrantFiled: December 18, 2019Date of Patent: May 16, 2023Assignee: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Adrianus Johannes Van Tunen
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Publication number: 20210207213Abstract: The invention relates to a kit of parts for detecting one or more polymorphisms in a plurality of target nucleotide sequences in a plurality of samples, wherein the kit of parts comprises (i) a first probe and a second probe, wherein the first probe comprises a first target specific section and a first tag section that is non-complementary to the target nucleotide sequence and that comprises a first universal primer binding site, wherein the second probe comprises a second target specific section and a second tag section that is non-complementary to the target nucleotide sequence; and (ii) a first primer and optionally a second primer wherein the first primer comprises a sample-specific identifier sequence and wherein the first primer can hybridize to the first universal primer binding site.Type: ApplicationFiled: March 22, 2021Publication date: July 8, 2021Applicant: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Rene Cornelis Josephus Hogers
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Publication number: 20210202035Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.Type: ApplicationFiled: March 11, 2021Publication date: July 1, 2021Applicant: KEYGENE N.V.Inventors: Michael Josephus Theresia VAN EIJK, Henricus Johannes Adam VAN DER POEL
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Publication number: 20210164021Abstract: The invention concerns a method for the production of oligonucleotides. The method of the invention uses a combination of amplification, restriction and affinity purification to produce high quality oligonucleotides. The invention further pertains to a nucleic acid precursor for use in the method of the invention, a solid support comprising said nucleic acid precursor and a kit for use in the method of the invention.Type: ApplicationFiled: December 10, 2020Publication date: June 3, 2021Applicant: Keygene N.V.Inventors: Rene Cornelis Josephus HOGERS, Maria Johanna BLEEKER, Michael Josephus Theresia VAN EIJK
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Patent number: 11008615Abstract: The invention relates to a kit for use in a method for detecting genetic variation in one or more members of a population, comprising (a) an adaptor adapted for ligation to a plurality of nucleic acid fragments and (b) a set of primers for PCR amplification having a 5?-end and a 3?-end, wherein at least one of the primers comprises one or more selective nucleotides at the 3? end, and wherein the adaptor and/or at least one of the primers comprises a sample-specific identifier sequence capable of indicating sample origin of an amplification product.Type: GrantFiled: July 19, 2019Date of Patent: May 18, 2021Assignee: KEYGENE N.V.Inventors: Michael Josephus Theresia Van Eijk, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek
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Patent number: 10988807Abstract: The invention relates to a kit of parts for detecting one or more polymorphisms in a plurality of target nucleotide sequences in a plurality of samples, wherein the kit of parts comprises (i) a first probe and a second probe, wherein the first probe comprises a first target specific section and a first tag section that is non-complementary to the target nucleotide sequence and that comprises a first universal primer binding site, wherein the second probe comprises a second target specific section and a second tag section that is non-complementary to the target nucleotide sequence; and (ii) a first primer and optionally a second primer wherein the first primer comprises a sample-specific identifier sequence and wherein the first primer can hybridize to the first universal primer binding site.Type: GrantFiled: August 13, 2019Date of Patent: April 27, 2021Assignee: KEYGENE N.V.Inventors: Michael Josephus Theresia Van Eijk, René Cornelis Josephus Hogers
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Patent number: 10978175Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.Type: GrantFiled: July 19, 2019Date of Patent: April 13, 2021Assignee: KEYGENE N.V.Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel
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Publication number: 20200291471Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.Type: ApplicationFiled: December 18, 2019Publication date: September 17, 2020Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Adrianus Johannes VAN TUNEN
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Publication number: 20200185056Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.Type: ApplicationFiled: July 19, 2019Publication date: June 11, 2020Applicant: KEYGENE N.V.Inventors: Michael Josephus Theresia VAN EIJK, Henricus Johannes Adam VAN DER POEL
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Publication number: 20200181698Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.Type: ApplicationFiled: July 19, 2019Publication date: June 11, 2020Applicant: KEYGENE N.V.Inventors: Michael Josephus Theresia VAN EIJK, Anker Preben SØRENSEN, Marco Gerardus Maria VAN SCHRIEK
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Publication number: 20200181694Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3? end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular marker.Type: ApplicationFiled: July 19, 2019Publication date: June 11, 2020Applicant: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Rene Cornelis Josephus Hogers
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Publication number: 20200102612Abstract: The present invention relates to a method for identifying the source of an amplicon, comprising: providing a plurality of pools of amplicons from different sources, wherein the amplicons from different sources are present in more than one pool, and wherein the amplicons in each pool are tagged with a unique pool-specific identifier; sequencing at least part of the amplicons that comprise the pool-specific identifiers; and assigning one or more of the amplicons to corresponding pools and/or sources using the pool-specific identifiers.Type: ApplicationFiled: May 17, 2019Publication date: April 2, 2020Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, Taco Peter JESSE, Adrianus Johannes VAN TUNEN
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Publication number: 20200071757Abstract: The invention relates to a method for the detection of a target nucleotide sequence in a sample based on an oligonucleotide ligation assay wherein probes are used that contain (a combination of) sequence-based identifiers that can identify the sample and the target sequence (i.e. locus and/or allele combination) wherein after the ligation step, the ligated probes, or after amplification, the amplified ligated probes, are restricted using restriction enzymes to cut of part of the probes and continue with those parts (identifiers and target sequence) that contain the relevant information in the sequencing step.Type: ApplicationFiled: August 13, 2019Publication date: March 5, 2020Applicant: Keygene N.V.Inventors: Michael Josephus Theresia VAN EIJK, René Cornelis Josephus HOGERS
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Patent number: 10538806Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.Type: GrantFiled: February 4, 2019Date of Patent: January 21, 2020Assignee: KEYGENE N.V.Inventors: Michael Josephus Theresia Van Eijk, Adrianus Johannes Van Tunen
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Patent number: 10422001Abstract: The invention relates to a method for the detection of a target nucleotide sequence in a sample based on an oligonucleotide ligation assay wherein probes are used that contain (a combination of) sequence-based identifiers that can identify the sample and the target sequence (i.e. locus and/or allele combination) wherein after the ligation step, the ligated probes, or after amplification, the amplified ligated probes, are restricted using restriction enzymes to cut of part of the probes and continue with those parts (identifiers and target sequence) that contain the relevant information in the sequencing step.Type: GrantFiled: August 22, 2017Date of Patent: September 24, 2019Assignee: KEYGENE N.V.Inventors: Michael Josephus Theresia Van Eijk, René Cornelis Josephus Hogers
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Patent number: 10316364Abstract: The present invention relates to a method for identifying the source of an amplicon, comprising: providing a plurality of pools of amplicons from different sources, wherein the amplicons from different sources are present in more than one pool, and wherein the amplicons in each pool are tagged with a unique pool-specific identifier; sequencing at least part of the amplicons that comprise the pool-specific identifiers; and assigning one or more of the amplicons to corresponding pools and/or sources using the pool-specific identifiers.Type: GrantFiled: September 18, 2017Date of Patent: June 11, 2019Assignee: KEYGENE N.V.Inventors: Michael Josephus Theresia Van Eijk, Taco Peter Jesse, Adrianus Johannes Van Tunen
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Publication number: 20190153534Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.Type: ApplicationFiled: February 4, 2019Publication date: May 23, 2019Applicant: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Adrianus Johannes Van Tunen
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Publication number: 20190144938Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.Type: ApplicationFiled: October 19, 2018Publication date: May 16, 2019Applicant: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek
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Publication number: 20190147977Abstract: The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples.Type: ApplicationFiled: November 1, 2018Publication date: May 16, 2019Applicant: Keygene N.V.Inventors: Michael Josephus Theresia Van Eijk, Henricus Johannes Adam Van Der Poel