Abstract: Disclosed is a method for producing genetically engineered cells in vitro. The method includes direct injection of a genome editing composition into a cell nucleus of a cell. The genome editing composition includes at least one Cas protein and at least one gRNA molecule to target a distinct genomic location. The injection is performed with a microelectromechanical systems injection chip including a cantilever. The cantilever includes a microchannel being in fluid communication with a nanosyringe, and wherein direct injection includes providing a fluid communication between the microchannel and the nucleus of the cell by insertion of the nanosyringe into the nucleus of the cell and injecting the genome editing composition via the microchannel through the nanosyringe into the nucleus of the cell.

Abstract: The invention relates to an HPDF operation method for an internal combustion engine (100) with internal formation of a mixture and self-ignition, in which, (i) for a combustion cycle of an operation cycle under high pressure, as main fuel (63) at a first time point, the introduction of a nonself-igniting or gasoline engine fuel, and as ignition fuel (64) at a second time point, the introduction of a self-igniting or diesel fuel into a combustion chamber (20) of the internal combustion engine (1) are at least initiated and/or performed, (ii) a self-ignition of the ignition fuel (64) and with the self-ignition a nonself-ignition of the main fuel (63) are effected, and (iii) the self-ignition of the ignition fuel (64) is performed temporally and/or spatially in such a way that the main fuel (63) is ignited at a location (1) and/or in a region of an jet tip (630 and/or a propagation front (630 of a quantity of introduced main fuel (63)—in particular temporally firstly.

Abstract: A parking lock for a transmission of a motor vehicle having an interlocking element of a connecting rod that is biased against a parking lock disengagement direction to bring and keep a ratchet tooth of a locking pawl in engagement with a tooth space of a parking interlock gear during an engagement of the parking lock. The connecting rod is provided a spring force acting in a parking lock engagement direction from an inserting spring and a compressive force acting in the parking lock disengagement direction from a hydraulically actuatable actuator having first piston pressurizable to disengage the parking lock. A detent device actuatable by an electromagnet mechanically fixes the first piston in either a latched or unlatched piston position associated with an engaged or disengaged position of the parking lock, respectively. A system determines a current engagement position of the detent device by assessing an inductance at the electromagnet.

Abstract: A parking lock for a transmission of a motor vehicle having an interlocking element of a connecting rod that is biased against a parking lock disengagement direction to bring and keep a ratchet tooth of a locking pawl in engagement with a tooth space of a parking interlock gear during an engagement of the parking lock. The connecting rod is provided a spring force acting in a parking lock engagement direction from an inserting spring and a compressive force acting in the parking lock disengagement direction from a hydraulically actuatable actuator having first piston pressurizable to disengage the parking lock. A detent device actuatable by an electromagnet mechanically fixes the first piston in either a latched or unlatched piston position associated with an engaged or disengaged position of the parking lock, respectively. A system determines a current engagement position of the detent device by assessing an inductance at the electromagnet.

Abstract: A motor vehicle transmission with a frictional parking lock mechanism is particularly suitable for shift-by-wire applications. The frictional parking lock mechanism makes it possible to have convenient controllability, as compared with positive parking lock mechanisms.

Abstract: A motor vehicle transmission with a frictional parking lock mechanism is particularly suitable for shift-by-wire applications. The frictional parking lock mechanism makes it possible to have convenient controllability, as compared with positive parking lock mechanisms.