Abstract: Compositions, kits and methods for detecting a plurality of targets are provided herein. A probe-set composition is provided, including one or more first probes and one or more second probes. Each of the first probe includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a first label, and a cleavage site for a first cleavage agent, wherein the first cleavage agent is capable of releasing the first label. Each of the second probes includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a second label, a quench moiety that renders the second label undetectable, and a cleavage site for the first cleavage agent. The first cleavage agent is capable of releasing the quench moiety, whereby the second label is rendered detectable.

Abstract: The present application provides certain advantageous ways of conducting multiplexed imaging.

Abstract: Compositions, kits and methods for detecting a plurality of targets are provided herein. A probe-set composition is provided, including one or more first probes and one or more second probes. Each of the first probe includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a first label, and a cleavage site for a first cleavage agent, wherein the first cleavage agent is capable of releasing the first label. Each of the second probes includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a second label, a quench moiety that renders the second label undetectable, and a cleavage site for the first cleavage agent. The first cleavage agent is capable of releasing the quench moiety, whereby the second label is rendered detectable.

Abstract: The present application provides certain advantageous ways of conducting multiplexed imaging.

Abstract: The present application provides certain advantageous ways of conducting multiplexed imaging.

Abstract: Compositions, kits and methods for detecting a plurality of targets are provided herein. A probe-set composition is provided, including one or more first probes and one or more second probes. Each of the first probe includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a first label, and a cleavage site for a first cleavage agent, wherein the first cleavage agent is capable of releasing the first label. Each of the second probes includes a nucleic acid sequence complementary to a nucleic acid barcode of a corresponding target-specific binding partner, a second label, a quench moiety that renders the second label undetectable, and a cleavage site for the first cleavage agent. The first cleavage agent is capable of releasing the quench moiety, whereby the second label is rendered detectable.

Abstract: A super-resolution fluorescence microscopy method comprises (a) providing a drift compensation marker, wherein (i) the drift compensation marker comprises a coated plasmonic particle; (ii) the drift compensation marker comprises an anisotropic plasmonic particle; and/or (iii) the drift compensation marker comprises a plasmonic particle comprising at least one reporter molecule on the surface of the plasmonic particle or on the surface of a coating; (b) performing super-resolution fluorescence microscopy; (c) using the drift compensation marker to improve the resolution of the super-resolution fluorescence microscopy.

Abstract: The present application provides compositions for multiplex imaging using labeled nucleic acid imaging agents.

Abstract: The present application provides compositions for multiplexed imaging using labeled nucleic acid imaging agents.

Abstract: The present application provides certain advantageous ways of conducting multiplexed imaging.