Patents by Inventor Michael Killoran

Michael Killoran has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12241839
    Abstract: Provided herein are bioluminescent polypeptides and compositions and methods for the assembly of a tripartite or multipartite bioluminescent complex. In particular embodiments, a bioluminescent complex is formed upon the interaction of three or more peptide and/or polypeptide components.
    Type: Grant
    Filed: November 27, 2020
    Date of Patent: March 4, 2025
    Assignee: Promega Corporation
    Inventors: Virginia Kincaid, Lance P. Encell, Mary Hall, Michael Killoran, Keith Wood, Melanie Dart, Trish Hoang
  • Publication number: 20240368565
    Abstract: Provided herein are compositions and systems comprising complementation-based tags and reporters for labeling and detection of targets by luminescence and a second modality (e.g., fluorescence), and methods of use thereof. In particular, tags are provided comprising the fusion of a first component of bioluminescent complex and a first component of modified dehalogenase complex, reporters are provided comprising the second component of bioluminescent complex and the second component of modified dehalogenase complex, and systems and methods are provided comprising the tags and reporters herein for dual-modality labeling and detection of targets.
    Type: Application
    Filed: May 3, 2024
    Publication date: November 7, 2024
    Inventors: Michael KILLORAN, Rahele Esmatpour, Lance P. Encell, Christopher Todd Eggers, Nina J. Bonde
  • Publication number: 20240344039
    Abstract: Provided herein are compositions and systems comprising a DNA polymerase domain, a thioredoxin binding domain (TBD), and thioredoxin (TRX), wherein one or both of the TRX and TBD are fused or otherwise conjugated to the DNA polymerase domain. A TRX or TBD may also be provided in a system herein as a separate entity (e.g., a binary system). The DNA polymerase/TBD/TRX compositions and systems herein are engineered to reduce stutter and/or to produce fewer stutter artifacts. Kits comprising the DNA polymerase/TBD/TRX compositions and systems herein and methods of use thereof are also within the scope herein.
    Type: Application
    Filed: March 4, 2024
    Publication date: October 17, 2024
    Inventors: Nicholas A. COURTNEY, Lance P. ENCELL, Robert A. MCLAREN, David Z. MOKRY, Kristopher ZIMMERMAN, Kimberly SCHNELL, Michael KILLORAN, Kimberly K. KNOCHE, Michael R. SLATER, Monika G. WOOD, Michael BROCKMAN
  • Publication number: 20230416699
    Abstract: Provided herein are enhanced luciferase enzymes for use with thermostable luciferin analogs for bioluminescent assays. In particular, the present disclosure provides compositions, assays, and methods for performing a bioluminescent assay using enhanced, high-activity luciferase enzymes compatible with thermostable luciferins, such as 5,5-disubstituted luciferin analogs.
    Type: Application
    Filed: June 16, 2023
    Publication date: December 28, 2023
    Inventors: Michael Killoran, Ce Shi, Mary Hall, Lance P. Encell, Thomas Kirkland
  • Patent number: 11767517
    Abstract: Provided herein are enhanced luciferase enzymes for use with thermostable luciferin analogs for bioluminescent assays. In particular, the present disclosure provides compositions, assays, and methods for performing a bioluminescent assay using enhanced, high-activity luciferase enzymes compatible with thermostable luciferins, such as 5,5-disubstituted luciferin analogs.
    Type: Grant
    Filed: June 5, 2019
    Date of Patent: September 26, 2023
    Assignee: Promega Corporation
    Inventors: Michael Killoran, Ce Shi, Mary Hall, Lance P. Encell, Thomas Kirkland
  • Publication number: 20210262941
    Abstract: Provided herein are bioluminescent polypeptides and compositions and methods for the assembly of a tripartite or multipartite bioluminescent complex. In particular embodiments, a bioluminescent complex is formed upon the interaction of three or more peptide and/or polypeptide components.
    Type: Application
    Filed: November 27, 2020
    Publication date: August 26, 2021
    Inventors: Virginia Kincaid, Lance P. Encell, Mary Hall, Michael Killoran, Keith Wood, Melanie Dart, Trish Hoang
  • Patent number: 10913972
    Abstract: Described are luciferin analogues, methods for making the analogues, kits including the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.
    Type: Grant
    Filed: July 19, 2019
    Date of Patent: February 9, 2021
    Assignee: Promega Corporation
    Inventors: Lance P. Encell, Mary P. Hall, Michael Killoran, Thomas A. Kirkland, Poncho Meisenheimer, Ce Shi
  • Publication number: 20200270586
    Abstract: Provided herein are compositions and methods for the assembly of a tripartite or multipartite bioluminescent complex. In particular, a bioluminescent complex is formed upon the interaction of two or more peptide tags (e.g., separately or fused as a dipeptide or tripeptide) and a polypeptide component.
    Type: Application
    Filed: June 12, 2019
    Publication date: August 27, 2020
    Inventors: Mary Hall, Lance P. Encell, Michael Killoran, Keith Wood, Thomas Smith, Virginia Kincaid, Thomas Kirkland, Melanie Dart
  • Publication number: 20200071682
    Abstract: Provided herein are enhanced luciferase enzymes for use with thermostable luciferin analogs for bioluminescent assays. In particular, the present disclosure provides compositions, assays, and methods for performing a bioluminescent assay using enhanced, high-activity luciferase enzymes compatible with thermostable luciferins, such as 5,5-disubstituted luciferin analogs.
    Type: Application
    Filed: June 5, 2019
    Publication date: March 5, 2020
    Inventors: Michael Killoran, Ce Shi, Mary Hall, Lance P. Encell, Thomas Kirkland
  • Publication number: 20190338340
    Abstract: Described are luciferin analogues, methods for making the analogues, kits including the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.
    Type: Application
    Filed: July 19, 2019
    Publication date: November 7, 2019
    Inventors: Lance P. Encell, Mary P. Hall, Michael Killoran, Thomas A. Kirkland, Poncho Meisenheimer, Ce Shi
  • Patent number: 10400264
    Abstract: Described are luciferin analogs, methods for making the analogs, kits including the analogs, and methods of using the compounds for the detection of luminescence in luciferase-based assays.
    Type: Grant
    Filed: December 1, 2017
    Date of Patent: September 3, 2019
    Assignee: PROMEGA CORPORATION
    Inventors: Lance P. Encell, Mary P. Hall, Michael Killoran, Thomas A. Kirkland, Poncho Meisenheimer, Ce Shi
  • Publication number: 20180155762
    Abstract: Described are luciferin analogues, methods for making the analogues, kits including the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.
    Type: Application
    Filed: December 1, 2017
    Publication date: June 7, 2018
    Inventors: Lance P. Encell, Mary P. Hall, Michael Killoran, Thomas A. Kirkland, Poncho Meisenheimer, Ce Shi