Patents by Inventor Michael Kubista
Michael Kubista has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9932633Abstract: The present description refers to methods and kits for the assessment of RNA quality in a sample. Stable RNA is used as a reference for the assessment of RNA quality, wherein the stable RNA has low susceptibility to nuclease degradation.Type: GrantFiled: March 20, 2013Date of Patent: April 3, 2018Assignee: Roche Molecular Systems, Inc.Inventors: Jens Bjoerkman, Michael Kubista
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Patent number: 9714448Abstract: The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA including the steps of (i) cultivation of a population of adherent cells in a cell culture vessel (ii) lysis of the population of adherent cells which is supposed to contain the target RNA in the sample vessel with a lysis buffer comprising between 0.05 M and 1 M of a chaotropic agent (iii) adding reagents to the sample vessel which are necessary to perform a reverse transcription reaction such that the chaotropic agent is present in a concentration of about 10 to 60 mM in the sample vessel, and reverse transcribing the target RNA and (iv) amplifying the first strand cDNA by means of subjecting the sample to multiple cycles of a thermocycling protocol.Type: GrantFiled: December 5, 2013Date of Patent: July 25, 2017Assignee: Roche Molecular Systems, Inc.Inventors: Michael Kubista, Linda Stroembom, Neven Zoric
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Publication number: 20160168631Abstract: The present invention is directed to a method for performing RT-PCR for amplifying a target RNA comprising the steps of a) lysis of a cellular sample which is supposed to contain the target RNA with a lysis buffer comprising between 0.2 M and 1 M Guanidine Thiocyanate, b) diluting the sample to an extend such that Guanidine Thiocyanate is present in a concentration of about 30 to 50 mM, e) reverse transcribing in the presence of a mixture of first strand cDNA synthesis primers, the mixture consisting of oligo dT primers and random primers, and d) subjecting the sample to multiple cycles of thermocycling protocol and monitoring amplification of the first strand cDNA in real time, characterized in that steps a) to c) are done in the same vessel.Type: ApplicationFiled: October 23, 2014Publication date: June 16, 2016Inventors: Martin Bengtsson, Michael Kubista, Anders Stahlberg, Linda Stroembom
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Patent number: 9200313Abstract: The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA comprising the steps of a) lysis of a cellular sample which is supposed to contain the target RNA with a lysis buffer comprising between 0.2 M and 1 M Guanidine Thiocyanate, b) diluting the sample to an extent such that Guanidine Thiocyanate is present in a concentration of about 30 to 50 mM, c) reverse transcribing in the presence of a mixture of first strand cDNA synthesis primers, the mixture consisting of oligo dT primers and random primers, and d) subjecting the sample to multiple cycles of a thermocycling protocol and monitoring amplification of the first strand cDNA in real time, characterized in that steps a) to c) are done in the same vessel.Type: GrantFiled: June 10, 2010Date of Patent: December 1, 2015Assignee: ROCHE MOLECULAR SYSTEMS, INC.Inventors: Martin Bengtsson, Michael Kubista, Anders Stahlberg, Linda Stroembom
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Publication number: 20150044685Abstract: The present description refers to methods and kits for the assessment of RNA quality in a sample. Stable RNA is used as a reference for the assessment of RNA quality, wherein the stable RNA has low susceptibility to nuclease degradation.Type: ApplicationFiled: March 20, 2013Publication date: February 12, 2015Inventors: Jens Bjoerkman, Michael Kubista
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Publication number: 20140212958Abstract: The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA including the steps of (i) cultivation of a population of adherent cells in a cell culture vessel (ii) lysis of the population of adherent cells which is supposed to contain the target RNA in the sample vessel with a lysis buffer comprising between 0.05 M and 1 M of a chaotropic agent (iii) adding reagents to the sample vessel which are necessary to perform a reverse transcription reaction such that the chaotropic agent is present in a concentration of about 10 to 60 mM in the sample vessel, and reverse transcribing the target RNA and (iv) amplifying the first strand cDNA by means of subjecting the sample to multiple cycles of a thermocycling protocol.Type: ApplicationFiled: December 5, 2013Publication date: July 31, 2014Applicant: ROCHE DIAGNOSTICS OPERATIONS INC.Inventors: Michael Kubista, Linda Stroembom, Neven Zoric
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Patent number: 8642266Abstract: The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA comprising the steps of a) lysis of a cellular sample which is supposed to contain the target RNA with a lysis buffer comprising between 0.2 M and 1 M guanidine thiocyanate, b) diluting the sample to an extend such that guanidine thiocyanate is present in a concentration of about 30 to 50 mM, c) reverse transcribing in the presence of a mixture of first strand cDNA synthesis primers, the mixture consisting of oligo dT primers and random primers, and d) subjecting the sample to multiple cycles of a thermocycling protocol and monitoring amplification of the first strand cDNA in real time.Type: GrantFiled: October 22, 2009Date of Patent: February 4, 2014Assignee: RocheDiagnostics Operations, Inc.Inventors: Martin Bergtsson, Michael Kubista, Anders Stahlberg, Linda Stroembom
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Patent number: 8623602Abstract: The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA including the steps of (i) cultivation of a population of adherent cells in a cell culture vessel (ii) lysis of the population of adherent cells which is supposed to contain the target RNA in the sample vessel with a lysis buffer comprising between 0.05 M and 1 M of a chaotropic agent (iii) adding reagents to the sample vessel which are necessary to perform a reverse transcription reaction such that the the chaotropic agent is present in a concentration of about 10 to 60 mM in the sample vessel, and reverse transcribing the target RNA and (iv) amplifying the first strand cDNA by means of subjecting the sample to multiple cycles of a thermocycling protocol.Type: GrantFiled: January 19, 2011Date of Patent: January 7, 2014Assignee: Roche Diagnostics Operations, Inc.Inventors: Michael Kubista, Linda Stroembom, Neven Zoric
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Publication number: 20110136180Abstract: The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA comprising the steps of a) lysis of a cellular sample which is supposed to contain the target RNA with a lysis buffer comprising between 0.2 M and 1 M Guanidine Thiocyanate, b) diluting the sample to an extend such that Guanidine Thiocyanate is present in a concentration of about 30 to 50 mM, c) reverse transcribing in the presence of a mixture of first strand cDNA synthesis primers, the mixture consisting of oligo dT primers and random primers, and d) subjecting the sample to multiple cycles of a thermocycling protocol and monitoring amplification of the first strand cDNA in real time, characterized in that steps a) to c) are done in the same vessel.Type: ApplicationFiled: June 10, 2010Publication date: June 9, 2011Inventors: Martin Bengtsson, Michael Kubista, Anders Stahlberg, Linda Stroembom
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Publication number: 20110111463Abstract: The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA including the steps of (i) cultivation of a population of adherent cells in a cell culture vessel (ii) lysis of the population of adherent cells which is supposed to contain the target RNA in the sample vessel with a lysis buffer comprising between 0.05 M and 1 M of a chaotropic agent (iii) adding reagents to the sample vessel which are necessary to perform a reverse transcription reaction such that the the chaotropic agent is present in a concentration of about 10 to 60 mM in the sample vessel, and reverse transcribing the target RNA and (iv) amplifying the first strand cDNA by means of subjecting the sample to multiple cycles of a thermocycling protocol.Type: ApplicationFiled: January 19, 2011Publication date: May 12, 2011Inventors: Michael Kubista, Linda Stroembom, Neven Zoric
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Publication number: 20100216194Abstract: The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA comprising the steps of a) lysis of a cellular sample which is supposed to contain the target RNA with a lysis buffer comprising between 0.2 M and 1 M guanidine thiocyanate, b) diluting the sample to an extend such that guanidine thiocyanate is present in a concentration of about 30 to 50 mM, c) reverse transcribing in the presence of a mixture of first strand cDNA synthesis primers, the mixture consisting of oligo dT primers and random primers, and d) subjecting the sample to multiple cycles of a thermocycling protocol and monitoring amplification of the first strand cDNA in real time.Type: ApplicationFiled: October 22, 2009Publication date: August 26, 2010Inventors: Martin Bergtsson, Michael Kubista, Anders Stahlberg, Linda Stroembom