Abstract: provided is a method for manufacturing a mold element for the production of microarrays, including the following steps: (i) providing a planar base element having a first surface and a second surface opposite the first surface, (ii) providing a planar auxiliary element on the second surface, (iii) penetrating the base element from the first surface in order to form mold openings, and (iv) reversibly or non-reversibly entering the auxiliary element when penetrating the base element. Moreover, a mold element for the production of microarrays, including a planar base element having a first surface a second surface opposite the first surface, a planar auxiliary element arranged on the second surface, and several mold openings extending from the surface of the base element through the second surface of the base element.

Abstract: The invention relates to a method for producing microstructures, the method including providing a planar mold element, which includes at least one mold opening for the microstructure to be produced, wherein the at least one mold opening has a first opening and a second opening; providing a first formulation at the second opening; generating a negative pressure in the mold opening; and taking up the first formulation through the second opening into the mold opening on account of the negative pressure in the mold opening. The invention further relates to a system for producing microstructures.

Abstract: An active ingredient means for delivering an active ingredient, in particular a pharmaceutical active ingredient, includes an active ingredient means and an electronic means cooperating therewith. The active ingredient means includes an active ingredient reservoir fluidically connected to a delivery means. The electronic means, which is manufactured in particular as a separate component, includes a control means and a pumping means electrically connected thereto. The pumping means is used to convey the active ingredient from the active ingredient reservoir to the delivery means.

Abstract: The invention relates to a method for producing a mold element for producing micro-arrays. First, a planar base element, in particular in the form of a film, is provided. Recesses are made in, in particular embossed into, an upper face of the element. The recesses are opened on a lower face of the base element in particular by using laser radiation. The invention also relates in particular to a mold element produced by means of this method, which mold element has recesses like the base element. Each of the recesses has openings in the lower face of the base element.

Abstract: A device for producing microstructures, particularly microneedles and more particularly microneedle arrays, including a female mold that has, on a top side, at least one in particular conical depressed portion for producing a microstructure. The female mold is, for example, in the form of a silicone cap and is connected to a hollow cylinder in particular via a holding element. A plunger is disposed movably inside the hollow cylinder.

Abstract: A microarray receptacle which has a first side and a second side. Furthermore, the microarray receptacle includes a carrier structure for connecting the microarray receptacle to an application point. To the carrier structure there is connected a carrier surface which in turn is connected to a microarray. An articulation device is arranged between the carrier surface and the carrier structure, the articulation device making possible movement of the microarray connected to the carrier surface relative to the carrier structure along the extent of the microarray. Further, a microarray receptacle cluster with a plurality of such microarray receptacles.

Abstract: The disclosure relates to an applicator the application of a microarray. The applicator includes a housing and a plunger that can move within said housing to accelerate the microarray. The applicator additionally includes a target force trigger, and said target force trigger secures the plunger in relation to the housing and releases the movement thereof when the target force is applied. The disclosure also relates to an application system for application of a microarray, including such an applicator and a microarray or a microarray receiving portion, preferably connected to a microarray receiving portion cluster, having a microarray. The microarray or the microarray receiving portion are arranged relative to the applicator and/or connected to the applicator such that the plunger is able to apply the microarray.

Abstract: A support for at least one microarray, with a housing and a punch arranged in a movable manner within the housing. At least one microarray is connected to the punch. The housing includes an application opening for applying the at least one microarray. Further, a method for producing a support for at least one microarray. The method includes the steps of manufacturing a housing with an application opening, manufacturing a punch, connecting a microarray to the punch, and arranging the punch in a movable manner within the housing.

Abstract: The present disclosure relates to a method for producing microneedle arrays in a mold, including a plurality of receptacles that taper from an upper base surface to a lower tip surface, where a first component is fed into at least two receptacles through a feed opening that is spaced apart from the base surface, where the receptacles are filled with an additional component from above the feed opening, where the fillings of at least said two receptacles, which fillings are formed of at least the first component and the additional component, are connected to one another above the base surfaces, and where after the first component and the additional component have solidified, the microneedle array comprising the fillings that have solidified to form needles is removed from the mold.

Abstract: A device for manufacturing microstructures including a permeable template, wherein the template comprises at least one recess for manufacturing the microstructures. In addition, the device comprises a vacuum-generating device connected to the template such that a vacuum is applied to at least one side of the template. Further, a method for filling a permeable template, wherein the template comprises at least one recess for manufacturing microstructures.

Abstract: The invention relates to methods that allow for the efficient differentiation to form pancreatic endoderm cells from pluripotent stem cells such as human embryonic stem cells and definitive endoderm cells. The invention is directly applicable to the ultimate generation of pancreatic beta cells that could be used as part of a therapy to treat or even cure diabetes. Additionally, the present invention may be used to generate liver endoderm cells from human embryonic stem cells and definite endoderm cells as well. This invention relates to a method for generating definitive endoderm and pancreatic endoderm cells from stem cells, preferably human embryonic stem cells using defined media in the absence of feeder cells. A simply two step procedure to provide pancreatic endoderm cells from embryonic stem cells represents further embodiments of the present invention.