Patents by Inventor Michael L. Nuccio
Michael L. Nuccio has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240099213Abstract: Provided here are methods of using a mutated patatin-like phospholipase II? (“pPLAII?,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAII? to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAII?. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.Type: ApplicationFiled: November 30, 2023Publication date: March 28, 2024Applicant: Syngenta Participations AGInventors: Timothy Kelliher, Satya Chintamanani, Brent Delzer, Michael L Nuccio, Robert Arthur Dietrich, Suresh Babu Kadaru, Todd Lee Warner, William Paul Bullock
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Publication number: 20240076683Abstract: Transgenic INIR20 soybean plants comprising modifications of the MON87701 soybean locus which provide for facile excision of the modified MON87701 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.Type: ApplicationFiled: October 4, 2023Publication date: March 7, 2024Inventors: Michael Lee Nuccio, Michael Andreas Kock, Joshua L. Price
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Publication number: 20240076684Abstract: Transgenic INIR19 soybean plants comprising modifications of the DAS81419 soybean locus which provide for facile excision of the modified DAS81419 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.Type: ApplicationFiled: October 4, 2023Publication date: March 7, 2024Inventors: Joshua L. Price, Michael Andreas Kock, Michael Lee Nuccio
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Patent number: 11840697Abstract: Provided are isolated cDNAs comprising a nucleotide sequence having at least 90% identity to SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53. Also provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit HI; methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid-inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.Type: GrantFiled: February 12, 2021Date of Patent: December 12, 2023Assignee: Syngenta Participations AGInventors: Timothy Kelliher, Satya Chintamanani, Brent Delzer, Michael L Nuccio, Robert Arthur Dietrich, Suresh Babu Kadaru, Todd Lee Warner, William Paul Bullock
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Publication number: 20220192120Abstract: Provided here are methods of using a mutated patatin-like phospholipase II? (“pPLAII?,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAII? to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAII?. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.Type: ApplicationFiled: March 10, 2022Publication date: June 23, 2022Applicant: Syngenta Participations AGInventors: Timothy Kelliher, Satya Chintamanani, Brent Delzer, Michael L Nuccio, Robert Arthur Dietrich, Suresh Babu KADARU, Todd Lee Warner, William Paul Bullock
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Patent number: 11304392Abstract: Provided here are methods of using a mutated patatin-like phospholipase II? (“pPLAII?,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAII? to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAII?. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.Type: GrantFiled: September 6, 2019Date of Patent: April 19, 2022Assignee: Syngenta Participations AGInventors: Timothy Kelliher, Satya Chintamanani, Brent Delzer, Michael L Nuccio, Robert Arthur Dietrich, Suresh Babu Kadaru, Todd Lee Warner, William Paul Bullock
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Patent number: 11261456Abstract: The present disclosure relates to regulatory sequences. In particular, the disclosure relates to a regulatory nucleic acid molecule, at least part of which has a transcription initiation function directing expression of an operably associated protein encoding polynucleotide of interest to non-tassel tissue in maize, but not or substantially not to tassel. The disclosure further relates to chimeric genes and expression cassettes comprising the regulatory nucleic acid molecule and to transgenic plants comprising the chimeric genes and expression cassettes.Type: GrantFiled: December 17, 2019Date of Patent: March 1, 2022Assignee: Syngenta Participations AGInventors: Mikyong Lee, Michael L. Nuccio, Joseph Clarke
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Publication number: 20210163971Abstract: Provided are isolated cDNAs comprising a nucleotide sequence having at least 90% identity to SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53. Also provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit HI; methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid-inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.Type: ApplicationFiled: February 12, 2021Publication date: June 3, 2021Applicant: Syngenta Participations AGInventors: Timothy Kelliher, Satya Chintamanani, Brent Delzer, Michael L Nuccio, Robert Arthur Dietrich, Suresh Babu KADARU, Todd Lee Warner, William Paul Bullock
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Patent number: 10954523Abstract: Provided are isolated cDNAs comprising a nucleotide sequence having at least 90% identity to SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53. Also provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit HI; methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid—inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.Type: GrantFiled: December 13, 2018Date of Patent: March 23, 2021Assignee: Syngenta Participations AGInventors: Timothy Kelliher, Satya Chintamanani, Brent Delzer, Michael L Nuccio, Robert Arthur Dietrich, Suresh Babu Kadaru, Todd Lee Warner, William Paul Bullock
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Publication number: 20200190528Abstract: The present invention relates to regulatory sequences. In particular, the invention relates to a regulatory nucleic acid molecule, at least part of which has a transcription initiation function directing expression of an operably associated protein encoding polynucleotide of interest to non-tassel tissue in maize, but not or substantially not to tassel. The invention further relates to chimeric genes and expression cassettes comprising the regulatory nucleic acid molecule and to transgenic plants comprising the chimeric genes and expression cassettes.Type: ApplicationFiled: December 17, 2019Publication date: June 18, 2020Applicant: SYNGENTA PARTICIPATIONS AGInventors: Mikyong Lee, Michael L. Nuccio, Joseph Clarke
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Publication number: 20200060110Abstract: Provided here are methods of using a mutated patatin-like phospholipase II? (“pPLAII?,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAII? to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAII?. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.Type: ApplicationFiled: September 6, 2019Publication date: February 27, 2020Applicant: Syngenta Participations AGInventors: Timothy Kelliher, Satya Chintamanani, Brent Delzer, Michael L Nuccio, Robert Arthur Dietrich, Suresh Babu Kadaru, Todd Lee Warner, William Paul Bullock
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Patent number: 10538777Abstract: The present invention relates to regulatory sequences. In particular, the invention relates to a regulatory nucleic acid molecule, at least part of which has a transcription initiation function directing expression of an operably associated protein encoding polynucleotide of interest to non-tassel tissue in maize, but not or substantially not to tassel. The invention further relates to chimeric genes and expression cassettes comprising the regulatory nucleic acid molecule and to transgenic plants comprising the chimeric genes and expression cassettes.Type: GrantFiled: October 5, 2017Date of Patent: January 21, 2020Assignee: Syngenta Participations AGInventors: Mikyong Lee, Michael L. Nuccio, Joseph Clarke
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Publication number: 20190390210Abstract: In one aspect, the present invention provides an expression cassette comprising a promoter operably linked to a nucleic acid molecule of interest, which is operably linked to a terminator, further comprising a heterologous intron that enhances expression of the nucleic acid molecule of interest, wherein the heterologous intron is selected from the group comprised of SEQUENCE ID NO. 1 (iZm10430-02), SEQUENCE ID NO. 2 (iZm10430-01) SEQUENCE ID NO. 3 (iZm008975-01), SEQUENCE ID NO. 4 (iZm005854-01), SEQUENCE ID NO. 5 (iZm010719-01), and SEQUENCE ID NO. 6 (iZm007840-01). In another aspect, the present invention provides an expression cassette comprising a promoter operably linked to a nucleic acid molecule of interest, which is operably linked to a heterologous terminator that enhances expression of the nucleic acid molecule of interest, wherein the heterologous terminator is selected from the group of SEQUENCE ID NO. 7 (tZmHSP70-01), SEQUENCE ID NO. 8 (tZmUbi158-01), SEQUENCE ID NO.Type: ApplicationFiled: July 10, 2019Publication date: December 26, 2019Inventors: Michael L. Nuccio, Jonathan Cohn
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Patent number: 10392627Abstract: In one aspect, the present invention provides an expression cassette comprising a promoter operably linked to a nucleic acid molecule of interest, which is operably linked to a terminator, further comprising a heterologous intron that enhances expression of the nucleic acid molecule of interest, wherein the heterologous intron is selected from the group comprised of SEQUENCE ID NO. 1 (iZm10430-02), SEQUENCE ID NO. 2 (iZm10430-01) SEQUENCE ID NO. 3 (iZm008975-01), SEQUENCE ID NO. 4 (iZm005854-01), SEQUENCE ID NO. 5 (iZm010719-01), and SEQUENCE ID NO. 6 (iZm007840-01). In another aspect, the present invention provides an expression cassette comprising a promoter operably linked to a nucleic acid molecule of interest, which is operably linked to a heterologous terminator that enhances expression of the nucleic acid molecule of interest, wherein the heterologous terminator is selected from the group of SEQUENCE ID NO. 7 (tZmHSP70-01), SEQUENCE ID NO. 8 (tZmUbi158-01), SEQUENCE ID NO.Type: GrantFiled: December 12, 2016Date of Patent: August 27, 2019Assignee: Syngenta Participations AGInventors: Michael L. Nuccio, Jonathan Cohn
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Publication number: 20190100763Abstract: Provided are isolated cDNAs comprising a nucleotide sequence having at least 90% identity to SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53. Also provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit HI; methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid—inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.Type: ApplicationFiled: December 13, 2018Publication date: April 4, 2019Applicant: Syngenta Participations AGInventors: Timothy Kelliher, Satya Chintamanani, Brent Delzer, Michael L. Nuccio, Robert Arthur Dietrich, Suresh Babu KADARU, Todd Lee Warner, William Paul Bullock
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Patent number: 10190125Abstract: Provided are isolated cDNAs comprising a nucleotide sequence having at least 90% identity to SEQ ID NO: 33, SEQ ID NO: 37, SEQ ID NO: 52 or SEQ ID NO: 53. Also provided are expression cassettes; vectors; transgenic plant cells; plants, plant parts, and seeds; isolated polypeptides; amplicons and informative fragments of the presently disclosed nucleic acids; compositions that include amplification primer pairs; methods for producing plants that exhibit HI; methods for identifying the presence or absence of an allele associated with HI in a plant; methods for introgressing Haploid-inducing nucleotide sequences into plants; and methods for selecting parental plants predicted to produce progeny generations with plants that exhibit Haploid Induction trait.Type: GrantFiled: May 4, 2017Date of Patent: January 29, 2019Assignee: Syngenta Participations AGInventors: Satya Chintamanani, Timothy Kelliher, Brent Delzer, Michael L. Nuccio, Robert Arthur Dietrich, Suresh Babu Kadaru, Todd Lee Warner, William Paul Bullock
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Patent number: 10006037Abstract: The present invention includes expression cassettes that contain regulatory sequences derived from a target gene, for example, regulatory sequences from the HSP70, Ubi158, and Ubi361 genes, for expression of recombinant gene products in plants. Developmental expression profiling data were used to identify several gene candidates for strong constitutive expression cassette development. Three expression cassettes were developed. They are based on the ZmHSP70, ZmUbi158, and ZmUBI361 genes.Type: GrantFiled: October 7, 2015Date of Patent: June 26, 2018Assignee: Syngenta Participations AGInventor: Michael L. Nuccio
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Publication number: 20180044691Abstract: The present invention relates to regulatory sequences. In particular, the invention relates to a regulatory nucleic acid molecule, at least part of which has a transcription initiation function directing expression of an operably associated protein encoding polynucleotide of interest to non-tassel tissue in maize, but not or substantially not to tassel. The invention further relates to chimeric genes and expression cassettes comprising the regulatory nucleic acid molecule and to transgenic plants comprising the chimeric genes and expression cassettes.Type: ApplicationFiled: October 5, 2017Publication date: February 15, 2018Applicant: Syngenta Participations AGInventors: Mikyong LEE, Michael L. NUCCIO, Joseph CLARKE
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Patent number: 9809826Abstract: The present invention relates to regulatory sequences. In particular, the invention relates to a regulatory nucleic acid molecule, at least part of which has a transcription initiation function directing expression of an operably associated protein encoding polynucleotide of interest to non-tassel tissue in maize, but not or substantially not to tassel. The invention further relates to chimeric genes and expression cassettes comprising the regulatory nucleic acid molecule and to transgenic plants comprising the chimeric genes and expression cassettes.Type: GrantFiled: August 7, 2015Date of Patent: November 7, 2017Assignee: Syngenta Participations AGInventors: Mikyong Lee, Michael L. Nuccio, Joseph Clarke
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Publication number: 20170268014Abstract: The present invention relates generally to the field of molecular biology and describes nucleic acids encoding regulatory elements capable of affecting expression of a coding sequence. The regulatory elements described herein may be used to direct the expression of a heterologous coding region in the green tissues and upon exposure to light in plants. The invention may also be used to create transgenic plants having improved characteristics, such as yield.Type: ApplicationFiled: December 8, 2015Publication date: September 21, 2017Applicant: Syngenta Participations AGInventor: Michael L. NUCCIO