Abstract: The disclosure relates to barcoded polymer nanoparticles for in vivo screening and for in vivo therapeutic delivery, and methods therefor. More particularly, the invention relates to polymer nanoparticles, such as reversible addition-fragmentation chain transfer (RAFT) polymer compositions, associated with polynucleotide barcodes, for therapeutic delivery, and for high throughput in vivo screening of drug delivery nanoparticles.

Abstract: The invention relates to barcoded nucleic acid nanostructure delivery compositions for in vivo screening for subsequent use in vivo therapeutic delivery, and methods therefor. More particularly, the invention relates to nucleic acid nanostructure delivery compositions, such as DNA origami structures, associated with barcodes for high throughput in vivo screening of the nucleic acid nanostructure delivery compositions for subsequent use in drug delivery, and methods therefor.

Abstract: A guidance system and method for keeping a tunnel boring, machine continuously on a plan line. The guidance system requires no machine operator calculations and provides the boring machine operator with a graphic display of past, present and projected positions of the boring machine from a horizontal and vertical perspective. The system uses a laser beam transmitter placed to the rear of the tunnel boring machine along with a front opaque target with a horizontal and vertical cross-hair and a rear transparent target with a horizontal and vertical cross-hair. The front and rear targets are disposed on the front and the rear of the boring machine. Also, an on-board programmable computer is installed on the boring machine for imputing data as to horizontal offset and vertical offset readings from the front and rear targets as the boring machine advances forward. Typically the boring machine moves forward in increments of four feet with offset readings taken by the operator after each increment.