Abstract: A system and method for optically detecting samples held in a solution requires the use of a holding plate that has as many as one-thousand through-hole wells, or more. The solution is suspended in these through-hole wells under surface tension between opposed surfaces of the holding plate. A pneumatic pump is then engaged with the plate to establish a differential pressure (&Dgr;p) between the upper and lower surfaces of the solution that is equal to approximately two tenths of a pound per square inch (0.2 psi). The result is the formation of a convex meniscus on a surface of the solution that causes light passing into the solution to converge and concentrate. This concentration of light, in turn, facilitates optical detection of samples in the solution.

Abstract: A holding plate includes a flat base member with a surface that is formed with a plurality of stations (wells or depressions) for holding specimens. A panel extends downwardly from the edge of the surface to create a concavity for receiving a lid member therein. The panel also has a flange extending outwardly from the concavity. The flat lid member has a skirt with a rim that extends from the edge of the lid member to create a hollow for receiving a base member therein. In operation, a robot or mechanical device selectively engages the flange of the base member and the rim of the lid member to engage or disengage the base and lid members. This allows for individual movement of either the base member or the lid member. This also allows for collective movement of the engaged base and lid members for stacking and storage.

Abstract: A holding plate for selectively heating and cooling samples in a solution has two opposing surfaces, and a plurality of cylindrically-shaped through-hole wells for holding the samples. Each well extends between the two surfaces of the holding plate, and has an aspect ratio of greater than 5:1, and a diameter less than approximately 500 microns. A metallic coating is applied by vapor deposition techniques on a surface of the holding plate. Importantly, this coating extends into each well through a distance of approximately one and a half well diameters for contact with the solution and the samples. A heat transfer device is thermally connected to the metallic coating for selectively heating and cooling the samples in the wells of the holding plate.

Abstract: Provided are methods of screening and identification of bioactivities and bioactive molecules of interest using a capillary array system. More specifically, disclosed are methods of using optical detection and capillary array-based techniques for screening libraries and recovering bioactive molecules having a desired activity or a nucleic acid sequence encoding such bioactive molecules.

Abstract: A sample screening platform, system and method for screening and recovery of detectable samples. The platform includes a capillary array including a plurality of capillaries. Each capillary includes at least one wall defining a lumen for retaining the detectable sample. The detectable sample is introduced to and retained in the lumen by capillary forces. In a method of incubating the sample, a second liquid is introduced into the capillary containing the sample. A detection and recovery system includes an optical system for detecting the sample, and a recovery mechanism adapted to contact a capillary containing the sample. The recovery mechanism is further adapted to recover the sample from the capillary, and expel the recovered sample for analysis.

Abstract: A device for positioning the tip of an elongated probe at a selected station of a holding plate includes motors to move the holding plate and a supporting stage within a coordinate plane (mxy). The elongated probe is also moveable along a probe axis that is oriented normal to the coordinate plane (mxy). A camera creates a pixel image of an optical marker placed on the stage. The image defines a coordinate plane (pxy). To relate the coordinate plane (pxy) to the coordinate plane (mxy), the optical marker is moved to successive locations in the mxy plane and a pixel image is obtained at each location. Using the pixel images, a computer calculates the relationship between coordinate planes and uses the relationship to signal the motors to move the holding plate in the mxy plane and position the selected station on the probe axis for interaction with the probe.

Abstract: Provided is a method of screening or enriching a sample containing polynucleotides from a mixed population of organisms. The method includes creating a DNA library from a plurality of nucleic acid sequences of a mixed population of organisms and separating clones containing a polynucleotide sequence of interest on an analyzer detects a detectable molecule on a probe or bioactive substrate. The analyzer includes FACS devices, SQUID devices and MCS devices. The separated or enrich library can then be further process by activity based screening or sequence based screening. In addition, the enriched sequence can be compared to a database and to identify sequences in the database which have homology to a clone in the library thereby obtaining a nucleic acid profile of the mixed population of organisms.

Abstract: Provided are methods of screening and identification of bioactivities and bioactive molecules of interest using a capillary array system. More specifically, disclosed are methods of using optical detection and capillary array-based techniques for screening libraries and recovering bioactive molecules having a desired activity or a nucleic acid sequence encoding such bioactive molecules.

Abstract: A sample screening platform, system and method for screening and recovery of detectable samples. The platform includes a capillary array including a plurality of capillaries. Each capillary includes at least one wall defining a lumen for retaining the detectable sample. The detectable sample is introduced to and retained in the lumen by capillary forces. In a method of incubating the sample, a second liquid is introduced into the capillary containing the sample. A detection and recovery system includes an optical system for detecting the sample, and a recovery mechanism adapted to contact a capillary containing the sample. The recovery mechanism is further adapted to recover the sample from the capillary, and expel the recovered sample for analysis.

Abstract: A sample screening platform, system and method for screening and recovery of detectable samples. The platform includes a capillary array including a plurality of capillaries. Each capillary includes at least one wall defining a lumen for retaining the detectable sample. The detectable sample is introduced to and retained in the lumen by capillary forces. In a method of incubating the sample, a second liquid is introduced into the capillary containing the sample. A detection and recovery system includes an optical system for detecting the sample, and a recovery mechanism adapted to contact a capillary containing the sample. The recovery mechanism is further adapted to recover the sample from the capillary, and expel the recovered sample for analysis.

Abstract: A device for positioning the tip of an elongated probe at a selected station of a holding plate includes motors to move the holding plate and a supporting stage within a coordinate plane (mxy). The elongated probe is also moveable along a linear probe axis that is orientated normal to the coordinate plane (mxy). A camera creates a pixel image of an optical marker placed on the stage. The image defines a coordinate plane (pxy). To relate the coordinate plane (pxy) to the coordinate plane (mxy), the optical marker is moved to successive locations in the mxy plane and a pixel image is obtained at each location. Using the pixel images, a computer calculates the relationship between coordinate planes and uses the relationship to signal the motors to move the holding plate in the mxy plane and position the selected station on the probe axis for interaction with the probe.

Abstract: A GigaMatrix plate for holding a large number of small-volume fluid samples includes a base for supporting a plurality of substantially parallel, elongated capillary tubes. Each tube defines a lumen that extends through the base, and each lumen has an aspect ratio greater than about 5:1. Dimensionally, each lumen has an inner diameter that is less than approximately five hundred microns and it has a length greater than about five millimeters. Further, each tube acts to optically distinguish light that is directed toward it from the sample whenever the sample fluoresces inside the tube lumen. Also, however, light from the sample that is directed axially through the tube is emitted therefrom for optical detection of the tube and the sample therein.

Abstract: A sample screening platform, system and method for screening and recovery of detectable samples. The platform includes a capillary array including a plurality of capillaries. Each capillary includes at least one wall defining a lumen for retaining the detectable sample. The detectable sample is introduced to and retained in the lumen by capillary forces. In a method of incubating the sample, a second liquid is introduced into the capillary containing the sample. A detection and recovery system includes an optical system for detecting the sample, and a recovery mechanism adapted to contact a capillary containing the sample. The recovery mechanism is further adapted to recover the sample from the capillary, and expel the recovered sample for analysis.

Abstract: A device, and a method for using the device, to generate discrete information about an environmental event includes an array of sensors. All sensors in the array have a determinable detection capability and, preferably, some of these detection capabilities are redundant while others are overlapping. The individual sensors are positioned in the particular environment to detect characteristics of the event from different perspectives. The outputs which are generated by the various sensors in the array are selectively segmented and joined to create a convolved pattern of data which explicitly and implicitly includes information about the characteristics of the event. The convolved pattern is then presented to a pattern recognition unit, such as a neural network, where the characteristics are interpreted from the convolved pattern to generate the desired discrete information about the environmental event.

Abstract: A one-piece wrap-around wrist guard is provided that includes a plurality of flexible plastic supports or stays positioned above and below the wrist. The wrap-around support structure helps absorb and distribute forces from a fall rather than transmitting them to the wrist, arm, elbow and shoulder.

Abstract: An ophthalmic lens inspection system includes an illumination assembly which generates diffuse light and transmits the diffuse light through an ophthalmic lens disposed in an inspection position, the diffuse light having a diffusivity of between 30.degree. and 50.degree.. An imaging assembly generates a set of signals representing selected portions of the diffuse light transmitted through the ophthalmic lens in the inspection position. A moving mechanism supports the imaging and illumination assemblies for common movement relative to the contact lens, to bring a reference point into alignment with the center of the imaging assembly.

Abstract: An ophthalmic lens inspection system includes an illumination assembly which generates diffuse light and transmits the diffuse light through an ophthalmic lens disposed in an inspection position, the diffuse light having a diffusivity of between 30.degree. and 50.degree.. An imaging assembly generates a set of signals representing selected portions of the diffuse light transmitted through the ophthalmic lens in the inspection position. A moving mechanism supports the imaging and illumination assemblies for common movement relative to the contact lens, to bring a reference point into alignment with the center of the imaging assembly.

Abstract: An optical fiber security system includes an optical emitter connected to one end of an optical fiber and a detector connected to the other end. A random signal generator triggers the emitter to output a light pulse signal through the fiber. This generator also simultaneously triggers the detector to receive the light pulse signal. A comparator compares the light pulse signal that is received by the detector with an optimum reference to adjust and conform the emitter output with the reference. Also, a monitor determines whether a particular identifiable characteristic of the light pulse signal is within a predetermined range of values. Whenever there is not a simultaneous emission and detection of the light pulse signal, or whenever the light pulse characteristic is outside the predetermined range of values, the system alarms.

Abstract: A hand-held portable arthroscope has a housing and a disposable cap removably attached to the distal end of the housing. A probe which includes a fiber optic image guide and a plurality of optical illuminating fibers is mounted on the cap, and is insertable into a body for imaging the internal structure of the body. The illuminating fibers extend through the cap and are connected in light communication with a quartz halogen lamp for illuminating the internal body structure being imaged. To gather light from the illuminated internal structure of a body into which the probe has been inserted, a GRIN rod is attached to the distal end of the image guide. Focussing optics are mounted in the housing in light communication with the image guide. These focussing optics are axially movable within the housing for focussing the image from the image guide. Light which passes through the focussing optics enters a camera head which is also mounted in the housing.

Abstract: A disposable arthroscope for examining the interior of a joint and for carrying out diagnostic and therapeutic procedures within the joint includes an elongated needle and a cap housing which has a base plate. The proximal end of the needle is mounted on the cap opposite the base plate, and the distal end of the needle extends outwardly from the cap. The needle is a hollow tube which surrounds an image guide and a bundle of illuminating fibers that extend through the tube. A lens is attached to the distal end of the image guide and is slightly angled relative to the longitudinal axis of the needle. The proximal ends of both the image guide and the bundle of illuminating fibers extend from the proximal end of the tube and through the cap for exposure at the surface of the base plate.