Abstract: Provided here are methods of using a mutated patatin-like phospholipase II? (“pPLAII?,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAII? to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAII?. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.

Abstract: The disclosure relates to improvements in methods for efficient editing of two or more endogenous tomato genes by insertion of one or more single stranded DNA donor molecules that are not homologous to the insertion sites in the tomato genome.

Abstract: Transgenic INIR19 soybean plants comprising modifications of the DAS81419 soybean locus which provide for facile excision of the modified DAS81419 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Transgenic INIR20 soybean plants comprising modifications of the MON87701 soybean locus which provide for facile excision of the modified MON87701 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Transgenic INIR11 maize plants comprising modifications of the MON89034 maize locus which provide for facile excision of the modified MON89034 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Transgenic INIR17 maize plants comprising an ecry3.1Ab expression cassette linked to a secondary nopaline synthase terminator element which lack a selectable marker gene and/or which comprise modifications that provide for facile excision of the INIR17 transgenic locus from the maize plant genome are provided. Genomic DNA of INIR17 transgenic plants, detection of INIR17 plants and products thereof, methods of making INIR17 plants, and use of INIR17 plants to facilitate breeding are disclosed.

Abstract: Transgenic INIR4 maize plants comprising modifications of the DAS59122-7 maize locus which provide for facile excision of the modified DAS59122-7 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Methods of selectively excising polynucleotide segments including segments comprising non-essential DNA and/or selectable marker genes from transgenic loci from transgenic plants as well as making targeted genetic changes with genome editing techniques are provided. Also provided are transgenic plants comprising the transgenic loci lacking the non-essential DNA and/or selectable markers and use of such methods and plants to facilitate plant breeding are disclosed.

Abstract: Transgenic plants comprising synthetic protospacer adjacent motifs (sPAMs) or synthetic guide RNA recognition sites introduced at or near the junctions the transgene insert with non-transgenic genomic DNA, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: The disclosure relates to precise modifications in wheat plant genomes including the heterologous integration of nucleic acid sequences that include a regulatory element at a predetermined locus. Also provided are wheat cells, wheat plants, wheat seeds, and processed wheat products comprising the modified wheat genomes.

Abstract: Transgenic INIR6 maize plants comprising modifications of the DP-4114 maize locus which provide for facile excision of the modified DP-4114 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Transgenic INHT31 soybean plants comprising modifications of the MON89788 soybean locus which provide for facile excision of the modified MON89788 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Transgenic INIR19 soybean plants comprising modifications of the DAS81419 soybean locus which provide for facile excision of the modified DAS81419 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Transgenic INIR20 soybean plants comprising modifications of the MON87701 soybean locus which provide for facile excision of the modified MON87701 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Transgenic INIR11 maize plants comprising modifications of the MON89034 maize locus which provide for facile excision of the modified MON89034 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: The disclosure relates to improvements in methods for efficient editing of two or more endogenous soybean genes by insertion of one or more single stranded DNA donor molecules that are not homologous to the insertion sites in the soybean genome.

Abstract: The disclosure relates to gene edited wheat plants, seeds, and compositions, as well as improvements to wheat plant breeding and methods for creating modifications in wheat plant genomes.

Abstract: Transgenic INHT31 soybean plants comprising modifications of the MON89788 soybean locus which provide for facile excision of the modified MON89788 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Plant cells and related systems, methods, and compositions for improving the capacity of the plant cells to regenerate embryogenic plant tissues, plant organs, and whole plants are provided. Such plant cells and related systems, methods, and compositions provide for increased expression of the endogenous morphoregulators BABYBOOM (ODP2) and/or WUSCHEL2 (WUS2).

Abstract: Transgenic INHT26 soybean plants comprising modifications of the DAS44406-6 soybean locus which provide for facile excision of the modified DAS44406-6 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.