Patents by Inventor Michael Levene
Michael Levene has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240135067Abstract: Systems and methods of generating an image of a modified desired cutting die are described herein. One image of a desired cutting die is received; an entire cutting edge of the desired cutting die in the image is identified, an image of a modified desired cutting die based on the offset distance and desired tolerance is generated. By using the systems and methods, The amount of material used to create a plurality of pieces of the material with a cutting machine is reduced.Type: ApplicationFiled: February 24, 2022Publication date: April 25, 2024Inventors: Merrick LEVENE, Michael R. GEFFROS, Wesley Elton SCOTT
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Publication number: 20240011977Abstract: The present invention relates to systems and methods for tissue processing and imaging including simultaneous dehydration and staining with fluorescent nuclear and protein dyes followed by optical sectioning microscopy.Type: ApplicationFiled: July 5, 2023Publication date: January 11, 2024Inventors: Michael Levene, Richard Torres
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Publication number: 20230204474Abstract: The present invention relates to systems and methods for tissue processing and analysis. Tissue chambers are configured to allow single-container chemical processing, imaging, and wax embedding of tissue samples in a single container without manipulation between steps. Tissue chambers with features to support the tissue sample and allow fluid flow between the tissue sample and the tissue chamber surface are disclosed. The features may be index matched to sample structures of interest or dissolvable in clearing solution to allow for in-chamber imaging with minimal distortion. Specialized tissue processing and wax removal apparatuses are also disclosed including for use with tissue chambers having frangible portions to permit ease of wax removal.Type: ApplicationFiled: January 13, 2023Publication date: June 29, 2023Inventors: Michael Levene, Richard Torres
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Patent number: 11573179Abstract: The present invention relates to systems and methods for tissue processing and imaging including a counterintuitive inverse relationship between protein dye concentration and tissue sample protein content. Varying dye concentration in such a manner affords higher quality fluorescent imaging at depth in tissue when using optical sectioning microscopy or second harmonic generation (SHG) analysis. Methods of the invention thereby provide more usable histopathology images while reducing waste and reagent costs and are of particular utility in combination assays that include simultaneous protein and nuclear staining and SHG analysis.Type: GrantFiled: August 24, 2020Date of Patent: February 7, 2023Assignee: APPLIKATE TECHNOLOGIES LLCInventors: Richard Torres, Michael Levene
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Patent number: 11555765Abstract: The present invention relates to systems and methods for tissue processing and analysis. Tissue chambers are configured to allow single-container chemical processing, imaging, and wax embedding of tissue samples in a single container without manipulation between steps. Tissue chambers with features to support the tissue sample and allow fluid flow between the tissue sample and the tissue chamber surface are disclosed. The features may be index matched to sample structures of interest or dissolvable in clearing solution to allow for in-chamber imaging with minimal distortion. Specialized tissue processing and wax removal apparatuses are also disclosed including for use with tissue chambers having frangible portions to permit ease of wax removal.Type: GrantFiled: January 6, 2020Date of Patent: January 17, 2023Assignee: APPLIKATE TECHNOLOGIES LLCInventors: Michael Levene, Richard Torres
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Publication number: 20210072156Abstract: The present invention relates to systems and methods for tissue processing and imaging including a counterintuitive inverse relationship between protein dye concentration and tissue sample protein content. Varying dye concentration in such a manner affords higher quality fluorescent imaging at depth in tissue when using optical sectioning microscopy or second harmonic generation (SHG) analysis. Methods of the invention thereby provide more usable histopathology images while reducing waste and reagent costs and are of particular utility in combination assays that include simultaneous protein and nuclear staining and SHG analysis.Type: ApplicationFiled: August 24, 2020Publication date: March 11, 2021Inventors: Richard Torres, Michael Levene
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Publication number: 20200371033Abstract: The invention provides improved systems and methods for multiphoton microscopy including pixel clocking techniques for minimizing pixel integration time and providing consistent signal intensity with maximized imaging speeds. Various systems and method are described for optimizing laser repetition rate based on dye lifetime, combining polygonal mirror scanning and stage translation, using the laser pulse signal to time pixel collection, and minimizing laser pulses and dye usage based on signal to background ratios.Type: ApplicationFiled: June 8, 2020Publication date: November 26, 2020Inventors: Michael Levene, Richard Torres
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Publication number: 20200348216Abstract: The present invention relates to systems and methods for tissue processing and analysis. Tissue chambers are configured to allow single-container chemical processing, imaging, and wax embedding of tissue samples in a single container without manipulation between steps. Tissue chambers with features to support the tissue sample and allow fluid flow between the tissue sample and the tissue chamber surface are disclosed. The features may be index matched to sample structures of interest or dissolvable in clearing solution to allow for in-chamber imaging with minimal distortion. Specialized tissue processing and wax removal apparatuses are also disclosed including for use with tissue chambers having frangible portions to permit ease of wax removal.Type: ApplicationFiled: January 6, 2020Publication date: November 5, 2020Inventors: Michael Levene, Richard Torres
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Patent number: 10677730Abstract: The invention provides improved systems and methods for multiphoton microscopy including pixel clocking techniques for minimizing pixel integration time and providing consistent signal intensity with maximized imaging speeds. Various systems and method are described for optimizing laser repetition rate based on dye lifetime, combining polygonal mirror scanning and stage translation, using the laser pulse signal to time pixel collection, and minimizing laser pulses and dye usage based on signal to background ratios.Type: GrantFiled: July 26, 2019Date of Patent: June 9, 2020Assignee: APLLIKATE TECHNOLOGIES LLCInventors: Michael Levene, Richard Torres
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Patent number: 10591392Abstract: A biopsy-sized tissue sample is stained for quick imaging. A significant amount of permeation enhancer is included in a mixed solution of permeant enhancer, fixative or dehydrant, and one or two fluorescent dyes to simultaneously dehydrate and dye the tissue sample. The permeation enhancer, e.g., 10% to 50% in the mixed solution, achieves an image of dyed tissue in the contacted tissue sample at a depth of at least 200 um within no more than 1.5 hours. One of the fluorescent dyes is a fluorescent nuclear dye such as DAPI, SYTOX green, acridine orange, propidium iodide, or a Hoechst dye. The other fluorescent dye is a fluorescent protein dye such as eosin or rhodamine B. The tissue sample is cleared with a clearing agent having a refractive index of at least 1.4[R2], e.g., using BABB. The mixed solution may further include Chloroform or other morphology preservative.Type: GrantFiled: July 3, 2014Date of Patent: March 17, 2020Assignee: APPLIKATE TECHNOLOGIES LLCInventors: Richard Torres, Michael Levene
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Patent number: 10527528Abstract: The present invention relates to systems and methods for tissue processing and analysis. Tissue chambers are configured to allow single-container chemical processing, imaging, and wax embedding of tissue samples in a single container without manipulation between steps. Tissue chambers with features to support the tissue sample and allow fluid flow between the tissue sample and the tissue chamber surface are disclosed. The features may be index matched to sample structures of interest or dissolvable in clearing solution to allow for in-chamber imaging with minimal distortion. Specialized tissue processing and wax removal apparatuses are also disclosed including for use with tissue chambers having frangible portions to permit ease of wax removal.Type: GrantFiled: October 18, 2018Date of Patent: January 7, 2020Assignee: APPLIKATE TECHNOLOGIES LLCInventors: Michael Levene, Richard Torres
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Publication number: 20190360901Abstract: The present invention relates to systems and methods for tissue processing and analysis. Tissue chambers are configured to allow single-container chemical processing, imaging, and wax embedding of tissue samples in a single container without manipulation between steps. Tissue chambers with features to support the tissue sample and allow fluid flow between the tissue sample and the tissue chamber surface are disclosed. The features may be index matched to sample structures of interest or dissolvable in clearing solution to allow for in-chamber imaging with minimal distortion. Specialized tissue processing and wax removal apparatuses are also disclosed including for use with tissue chambers having frangible portions to permit ease of wax removal.Type: ApplicationFiled: October 18, 2018Publication date: November 28, 2019Inventors: Michael Levene, Richard Torres
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Publication number: 20160003716Abstract: The present invention includes novel methods of processing a tissue sample. The present invention also includes novel methods of imaging a tissue sample. The present invention further includes a specimen holding device for performing the novel methods of processing a tissue sample and a microscope system for performing the novel methods of imaging a tissue sample.Type: ApplicationFiled: July 2, 2015Publication date: January 7, 2016Inventors: Richard Torres, Michael Levene
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Publication number: 20160003715Abstract: The present invention includes novel methods of processing a tissue sample. The present invention also includes novel methods of imaging a tissue sample.Type: ApplicationFiled: July 3, 2014Publication date: January 7, 2016Inventors: Richard Torres, Michael Levene
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Patent number: 7943307Abstract: The present invention is directed to a method of sequencing a target nucleic acid. The method provides a complex comprising a polymerase enzyme, a target nucleic acid molecule, and a primer, wherein the complex is immobilized on a support Fluorescent label is attached to a terminal phosphate group of the nucleotide or nucleotide analog. The growing nucleic acid strand is extended by using the polymerase to add a nucleotide analog to the nucleic acid strand. The nucleotide analog added to the oligonucleotide primer as a result of the polymerizing step is identified. The time duration of the signal from labeled nucleotides or nucleotide analogs that become incorporated is distinguished from freely diffusing labels by a longer retention in the observation volume for the nucleotides or nucleotide analogs that become incorporated than for the freely diffusing labels.Type: GrantFiled: January 19, 2006Date of Patent: May 17, 2011Assignee: Cornell Research FoundationInventors: Jonas Korlach, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet
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Patent number: 7943305Abstract: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid. Each type of labeled nucleotide comprises an acceptor fluorophore attached to a phosphate portion of the nucleotide such that the fluorophore is removed upon incorporation into a growing strand. Fluorescent signal is emitted via fluorescent resonance energy transfer between the donor fluorophore and the acceptor fluorophore as each nucleotide is incorporated into the growing strand. The sequence is deduced by identifying which base is being incorporated into the growing strand.Type: GrantFiled: April 13, 2006Date of Patent: May 17, 2011Assignee: Cornell Research FoundationInventors: Jonas Korlach, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet
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Publication number: 20110111401Abstract: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site.Type: ApplicationFiled: July 22, 2010Publication date: May 12, 2011Applicant: Cornell UniversityInventors: Jonas KORLACH, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet
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Publication number: 20090137007Abstract: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site.Type: ApplicationFiled: November 5, 2008Publication date: May 28, 2009Inventors: Jonas Korlach, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet
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Patent number: 7485424Abstract: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site.Type: GrantFiled: March 21, 2006Date of Patent: February 3, 2009Assignee: Cornell Research Foundation, Inc.Inventors: Jonas Korlach, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet
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Publication number: 20080227654Abstract: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonuelcotide primer at an active site.Type: ApplicationFiled: February 6, 2008Publication date: September 18, 2008Inventors: Jonas Korlach, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet