Abstract: Golf balls having dimple patterns arranged in dipyramid layouts are disclosed. The patterns may be arranged in triangular dipyramid, quadrilateral dipyramid, pentagonal dipyramid, or hexagonal dipyramid layouts. The dipyramid patterns have six, eight, ten, or twelve substantially identical dimple sections, where each dimple section is defined by a spherical triangle. The dimples in each of the identical dimple sections have at least two different dimple diameters including a minimum dimple diameter and a maximum dimple diameter. The resulting dimple patterns have a surface coverage of about 70 percent or less. The reduced surface coverage helps to reduce the flight of the golf balls.

Abstract: Golf balls having a dimple pattern arranged in a tetrahedral layout are disclosed. The dimple pattern has four substantially identical dimple sections, where each dimple section is defined by a spherical triangle. The dimples in each of the four dimple sections have at least three different dimple diameters including a minimum dimple diameter, a maximum dimple diameter, and at least one additional dimple diameter. The resulting dimple pattern has a surface coverage of about 70 percent or less. The reduced surface coverage helps to reduce the flight of the golf balls.

Abstract: Golf balls having dimple patterns arranged in dipyramid layouts are disclosed. The patterns may be arranged in triangular dipyramid, quadrilateral dipyramid, pentagonal dipyramid, or hexagonal dipyramid layouts. The dipyramid patterns have six, eight, ten, or twelve substantially identical dimple sections, where each dimple section is defined by a spherical triangle. The dimples in each of the identical dimple sections have at least two different dimple diameters including a minimum dimple diameter and a maximum dimple diameter. The resulting dimple patterns have a surface coverage of about 70 percent or less. The reduced surface coverage helps to reduce the flight of the golf balls.

Abstract: Golf balls having a dimple pattern arranged in a tetrahedral layout are disclosed. The dimple pattern has four substantially identical dimple sections, where each dimple section is defined by a spherical triangle. The dimples in each of the four dimple sections have at least three different dimple diameters including a minimum dimple diameter, a maximum dimple diameter, and at least one additional dimple diameter. The resulting dimple pattern has a surface coverage of about 70 percent or less. The reduced surface coverage helps to reduce the flight of the golf balls.

Abstract: Golf balls having a dimple pattern arranged in an icosahedral layout are disclosed. The dimple pattern has 20 substantially identical dimple sections, where each dimple section is defined by a spherical triangle. The dimples in each of the 20 dimple sections have at least three different dimple diameters including a minimum dimple diameter, a maximum dimple diameter, and at least one additional dimple diameter. The resulting dimple pattern has a surface coverage of about 70 percent or less. The reduced surface coverage helps to reduce the flight of the golf balls.

Abstract: Golf balls having a dimple pattern arranged in an octahedral layout are disclosed. The dimple pattern has eight substantially identical dimple sections, where each dimple section is defined by a spherical triangle. The dimples in each of the eight dimple sections have at least three different dimple diameters including a minimum dimple diameter, a maximum dimple diameter, and at least one additional dimple diameter. The resulting dimple pattern has a surface coverage of about 70 percent or less. The reduced surface coverage helps to reduce the flight of the golf balls.

Abstract: A method of testing for discerning substitution of carbohydrate ester includes the step of providing a predetermined amount of a solution. Further, the method also includes adding ammonium hydroxide and sodium borohydride to the solution. The method also includes the step of transferring the solution to an ammonium ion exchange cartridge and collecting the eluate from the cartridge. Also included in the method is the step of analyzing the sample in a mass spectrometer to produce a mass spectrum. Further, the method includes calculating the amu of phosphate and sulfate substitution of the ion and comparing it to the actual amu of the found ion.

Abstract: A method for isolating derivatives of general O-linked amino acids including derivatives of O-tyrosine linked kappa casein di-O-sulfo tetrasaccharide and of O-di-phospho serine linked bovine submaxillary mucin disaccharide is provided. These derivatives are isolated and analyzed by further enzymatically separating the N-linked oligosaccharides from the O-linked oligosaccharides and then cleave O-linked oligosaccharides by decreasing the pH levels such that de-amidations are allowed. This de-amidation results in individual O-linked glycan amino acid components removed from the original glycoprotein. The O-linked glycan amino acid components are thus isolated from the glycoprotein, and can be individually analyzed.

Abstract: A laboratory method used for isolating and identifying fruit oligosaccharides from ripe banana fruit is provided. The method includes the step of obtaining a predetermined amount of a plant, such as banana fruit and combining it with ethanol. The method also includes the step of isolating an oligosaccharide from the plant without adding heat to the ethanol. The method can be used to isolate and identify neokestose-1,6-diphosphate.

Abstract: Methods for making designed carbohydrate affinity columns and lectin columns are provided. Reducing sugar of choice is dissolved in pH 6.0 acetate buffer and injected onto a primary amino group functionality column. The column is washed with pH 6.0 acetate buffer and the column effluent monitored with a variable wavelength UV detector. Lectin columns are made by injecting appropriate lectin to the designed carbohydrate and the lectin binds to this scaffold. The lectin column is formed.

Abstract: A laboratory method and system used to isolate and analyze O-linked oligosaccharides from glycoproteins that uses non-reductive ?-elimination (NBRE). The method including the step of providing a predetermined amount of solution and then passing it through an ion exchange resin. The method further includes collecting a second solution off of the ion exchange resin and adding a predetermined amount of sodium hydroxide to form a third solution. The third solution is allowed to stand for a set amount of time at a particular temperature. The third solution is then washed through an ion exchange cartridge in the ammonium form. The collected fourth solution is evaporated and pushed through a sodium form resin prior to being analyzed for its composition.

Abstract: A laboratory method and system used to isolate and analyze O-linked oligosaccharides from glycoproteins that uses non-reductive ?-elimination (NBRE). The method including the step of providing a predetermined amount of solution and then passing it through an ion exchange resin. The method further includes collecting a second solution off of the ion exchange resin and adding a predetermined amount of sodium hydroxide to form a third solution. The third solution is allowed to stand for a set amount of time at a particular temperature. The third solution is then washed through an ion exchange cartridge in the ammonium form. The collected fourth solution is evaporated and pushed through a sodium form resin prior to being analyzed for its composition.

Abstract: A laboratory method used for isolating and identifying fruit oligosaccharides from ripe banana fruit is provided. The method includes the step of obtaining a predetermined amount of a plant, such as banana fruit and combining it with ethanol. The method also includes the step of isolating an oligosaccharide from the plant without adding heat to the ethanol. The method can be used to isolate and identify neokestose-1,6-diphosphate.

Abstract: A method of testing for a trisaccharide in milk includes the step of providing a predetermined amount of milk. The method also includes the step of isolating a trisachharide of the milk with a hydride insertion reaction to identify the molecule as phosphorylated. Also included in the method is the step of obtaining a mass spectrum of the isolated milk trisaccharide sample. The method further includes the step of identifying a molecular structure of the isolated milk trisaccharide sample.

Abstract: A method of testing for discerning substitution of carbohydrate ester includes the step of providing a predetermined amount of a solution. Further, the method also includes adding ammonium hydroxide and sodium borohydride to the solution. The method also includes the step of transferring the solution to an ammonium ion exchange cartridge and collecting the eluate from the cartridge. Also included in the method is the step of analyzing the sample in a mass spectrometer to produce a mass spectrum. Further, the method includes calculating the amu of phosphate and sulfate substitution of the ion and comparing it to the actual amu of the found ion.

Abstract: A method of forming methanol, formaldehyde, formic acid and ammonium pentaborate tetrahydrate includes the steps of providing ammonium hydroxide and producing air bubbles within the ammonium hydroxide to form a solution. Sodium borohydride is added and dissolved within the solution of air bubbled ammonium hydroxide to form methanol, formaldehyde, formic acid and ammonium pentaborate tetrahydrate. An alternative method of forming methanol, formaldehyde, formic acid and ammonium pentaborate tetrahydrate is also provided which includes the steps of providing ammonium hydroxide and dissolving sodium borohydride therein to form a solution. Sodium bicarbonate is added to the solution of ammonium hydroxide and sodium borohydride to form methanol, formaldehyde, formic acid and ammonium pentaborate tetrahydrate.

Abstract: Methods for making designed carbohydrate affinity columns and lectin columns are provided. Reducing sugar of choice is dissolved in pH 6.0 acetate buffer and injected onto a primary amino group functionality column. The column is washed with pH 6.0 acetate buffer and the column effluent monitored with a variable wavelength UV detector. Lectin columns are made by injecting appropriate lectin to the designed carbohydrate and the lectin binds to this scaffold. The lectin column is formed.

Abstract: A method of forming methanol formaldehyde, formic acid and ammonium pentaborate tetrahydrate includes the steps of providing ammonium hydroxide and producing air bubbles within the ammonium hydroxide to form a solution. Sodium borohydride is added and dissolved within the solution of air bubbled ammonium hydroxide to form methanol formaldehyde, formic acid and ammonium pentaborate tetrahydrate. An alternative method of forming methanol formaldehyde, formic acid and ammonium pentaborate tetrahydrate is also provided which includes the steps of providing ammonium hydroxide and dissolving sodium borohydride therein to form a solution. Sodium bicarbonate is added to the solution of ammonium hydroxide and sodium borohydride to form methanol formaldehyde, formic acid and ammonium pentaborate tetrahydrate.

Abstract: A method for isolating banana starch from green bananas where the green banana is blended with a solution of sodium bisulfite and water. The liquid suspension of the resulting mixture is then centrifuged to separate the green material and the white banana starch. The white banana starch is then washed in a 95% ethanol to wash free the sodium bisulfite and isolate the banana starch.

Abstract: A method of testing for a trisaccharide in milk includes the step of providing a predetermined amount of milk. The method also includes the step of isolating a trisachharide of the milk with a hydride insertion reaction to identify the molecule as phosphorylated. Also included in the method is the step of obtaining a mass spectrum of the isolated milk trisaccharide sample. The method further includes the step of identifying a molecular structure of the isolated milk trisaccharide sample.