Abstract: The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.

Abstract: Methods, systems, processes, and apparatuses are provided for delivery across cell membranes. In one aspect, an apparatus includes a substrate including a mixing channel, a process chamber, and a dilution channel to perform delivery of the payload to across the cell membranes. In another aspect, a system includes reservoirs for a cell suspension, a delivery solution, and a stop solution connected to a pump. The system further includes an agitator, a heater, a temperature controller, and a controller to operate the system. In yet another aspect, cells in suspension are mixed with a delivery solution in a microfluidic mixing chip. The delivery solution includes a permeabilization agent to cause permeabilization of the cells, allowing delivery of a payload from the delivery solution to the cells across the cell membranes.

Abstract: Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.

Abstract: Methods, systems, processes, and apparatuses are provided for delivery across cell membranes. In one aspect, an apparatus includes a substrate including a mixing channel, a process chamber, and a dilution channel to perform delivery of the payload to across the cell membranes. In another aspect, a system includes reservoirs for a cell suspension, a delivery solution, and a stop solution connected to a pump. The system further includes an agitator, a heater, a temperature controller, and a controller to operate the system. In yet another aspect, cells in suspension are mixed with a delivery solution in a microfluidic mixing chip. The delivery solution includes a permeabilization agent to cause permeabilization of the cells, allowing delivery of a payload from the delivery solution to the cells across the cell membranes.

Abstract: The present subject matter provides a method for delivering a gene editing composition across a plasma membrane of a cell. Related apparatus, system, techniques, compositions, and articles are also described.

Abstract: The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.

Abstract: The invention provides a solution to the problem of transfecting non-adherent cells. Methods and compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to mammalian cells.

Abstract: The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.

Abstract: The current subject matter provides a cell engineering platform for vector-free and/or viral delivery of payload/cargo compounds and compositions into cells. The platform achieves delivery to cells quickly and in an easy to use manner. Related apparatus, systems, techniques, articles and compositions are also described.

Abstract: This invention relates to a roof tile comprising a top surface, a bottom surface, a pair of side edges and a pair of end edges. The roof tile comprises an overlapping portion for overlapping an adjacent roof tile and an underlapping portion for underlapping another adjacent roof tile. The overlapping portion comprises a solar panel forming the top surface and there are provided electrical connectors in communication with the solar panel for connection of the solar panel to a household power supply system. Ideally, a plurality of the roof tiles may be connected together to provide more electricity and each roof tile is provided with connectors for connection of the roof tile to other roof tiles. In this way, the solar panel will be integrated seamlessly into the roof, will not be proud of the roof and will not require a frame for mounting on the roof.

Abstract: The present subject matter provides a method for delivering a gene editing composition across a plasma membrane of a cell. Related apparatus, system, techniques, compositions, and articles are also described.

Abstract: An apparatus includes a catheter, and an electrode and methods of delivering molecules to eukaryotic cells using such an apparatus. The catheter defines a fluidic channel and has a distal opening. The electrode is within the fluidic channel and is spaced a distance from the distal opening of the catheter. The catheter is arranged to prevent direct contact between any electrode of the apparatus and tissue. Related apparatus, systems, techniques and articles are also described.

Abstract: Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.

Abstract: The invention provides a method of viral transfection of cells.

Abstract: Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.

Abstract: The invention provides a solution to the problem of transfecting non-adherent cells. Devices and delivery compositions containing ethanol and an isotonic salt solution are used for delivery of compounds and compositions to non-adherent cells.

Abstract: Delivering a payload across a plasma membrane of a cell includes providing a population of cells and contacting the population of cells with a volume of an aqueous solution. The aqueous solution includes the payload and alcohol content greater than 5 percent concentration. The volume of the aqueous solution may be a function of exposed surface area of the population of cells, or may be a function of a number of cells in the population of cells. Related compositions, apparatus, systems, techniques, and articles are also described.

Abstract: The invention relates to methods of engineering cells (e.g., dendritic cells (DCs)) for vaccinations (e.g., COVID-19) using ethanol-based transient cell membrane permeabilization. Related methods, compositions, apparatus, systems, and articles as described and/or illustrated herein.

Abstract: The current subject matter provides a cell engineering platform of a diagnostic and clinical use scale for vector-free and/or viral delivery of payload/cargo compounds and compositions into non-adherent cells. The platform achieves delivery to a large number of cells quickly in a closed system. Related apparatus, systems, techniques, articles and compositions are also described.

Abstract: An apparatus for use with a barrier having an overhanging ledge to limit movement by a person on the ledge. The apparatus has an upright member that is positioned next to the barrier, a base member at a lower end of the upright member that is positioned on a surface adjacent the barrier and opposite the ledge, a mechanism for engaging the barrier to hold the apparatus in place and a plurality of limiting members that extend across the ledge to prevent movement past the apparatus. In one configuration, each of the limiting members extend through openings in a railing above the barrier. The engaging mechanism defines a gap that receives the barrier to hold the apparatus to the barrier. The base member can have a footplate on which weight can be placed. A system has a pair of apparatuses that define an area in which movement is allowed.