Abstract: The present invention provides methods for bioprinting collagen and extracellular matrix-based bioinks, and articles such as engineered tissues, comprising the bioinks. In other aspects, the present invention relates to a bioprinting system including a 3D bioprinter with a nozzle, at least one bioink, a support bath solution, wherein the support bath solution comprises PEG (polyethylene glycol) and agarose.

Abstract: A method of generating thickened collagen bundles or clusters, comprising the steps of: preparing a neutralized collagen gel sample; warming the neutralized collagen gel sample to provide a warmed neutralized collagen gel sample; and agitating the warmed neutralized collagen gel sample to provide an agitated collagen gel sample.

Abstract: In some aspects, the present invention provides a method of forming crowded collagen based constructs, having the steps of providing a collagen solution in a buffer, providing a macromolecular bath solution, and delivering the collagen solution into the macromolecular bath solution to form crowded collagen constructs. In other aspects, the present invention provides a method of forming crowded cellular based constructs, having the steps of providing a cellular solution in a buffer, providing a macromolecular bath solution, and delivering the cellular solution into the macromolecular bath solution to form crowded cellular constructs.

Abstract: The present invention relates to a microfluidic device, a microfluidic system, and methods for tracking single cells, multiple cells, single cell lineages, and multiple cell lineages in series and/or in parallel. The microfluidic device comprises a substrate having one microfluidic channel formed therein or a plurality of microfluidic channels formed therein and arranged in parallel. The microfluidic system comprises: a microfluidic device according to the present invention; a cell loading reservoir in fluid communication with the inlet end of each microfluidic channel of the microfluidic device; and an outlet reservoir in fluid communication with the outlet end of each microfluidic channel of the microfluidic device. The present invention also relates to a high throughput microfluidic system and a kit for tracking single cells and/or single cell lineages.

Abstract: The present invention relates to a microfluidic device, a microfluidic system, and methods for tracking single cells, multiple cells, single cell lineages, and multiple cell lineages in series and/or in parallel. The microfluidic device comprises a substrate having one microfluidic channel formed therein or a plurality of microfluidic channels formed therein and arranged in parallel. The microfluidic system comprises: a microfluidic device according to the present invention; a cell loading reservoir in fluid communication with the inlet end of each microfluidic channel of the microfluidic device; and an outlet reservoir in fluid communication with the outlet end of each microfluidic channel of the microfluidic device. The present invention also relates to a high throughput microfluidic system and a kit for tracking single cells and/or single cell lineages.

Abstract: A check-in method for airlines allows passengers to use Short Message Service (SMS) to check-in themselves and baggage. A passenger requests to check-in via SMS from practically anywhere and not limited to the airport. After receipt of the request, a confirmation message is sent back to the passenger's registered mobile phone number by SMS. The system will ask the passenger for, and receive from the passenger, the number of bags they intend to check-in for the flight. The passengers can in some embodiments drop the baggage off without any required further action. The system issues an SMS-based cryptographically secure code, such as a bar-code boarding pass to the passenger once the baggage (if any) is successfully transferred and registered to existing baggage management systems through the process above. This code, when scanned, recognized and authenticated provides identification and security clearance for the passenger to board the aircraft.

Abstract: A check-in method for airlines allows passengers to use Short Message Service (SMS) to check-in themselves and baggage. A passenger requests to check-in via SMS from practically anywhere and not limited to the airport. After receipt of the request, a confirmation message is sent back to the passenger's registered mobile phone number by SMS. The system will ask the passenger for, and receive from the passenger, the number of bags they intend to check-in for the flight. The passengers can in some embodiments drop the baggage off without any required further action. The system issues an SMS-based encrypted code, such as a bar-code boarding pass to the passenger once the baggage (if any) is successfully transferred and registered to existing baggage management systems through the process above. This code, when scanned, recognized and authenticated provides identification and security clearance for the passenger to board the aircraft.