Abstract: The invention relates to a material which consists essentially of sand and polymers as well as optionally, a small quantity of aggregates. The invention also relates to a method for producing this material, comprising the following steps: producing a hot molten mixture consisting of sand and polymers; introducing said molten mixture into a mould; cooling the molten mixture under pressure until the molten mixture hardens in the mould. The material can be used e.g. for producing building blocks, pavement slabs, manhole covers, housings in electrical engineering and insulators for high-voltage applications.

Abstract: The invention relates to a material which consists essentially of sand and polymers as well as optionally, a small quantity of aggregates. The invention also relates to a method for producing this material, comprising the following steps: producing a hot molten mixture consisting of sand and polymers; introducing said molten mixture into a mould; cooling the molten mixture under pressure until the molten mixture hardens in the mould. The material can be used e.g. for producing building blocks, pavement slabs, manhole covers, housings in electrical engineering and insulators for high-voltage applications.

Abstract: The present invention utilizes AAV as a vector to transfect epithelial cells with an AAV/heterologous gene containing/Neo vector, to form a heterologous protein secreting culture of epithelial cells. This culture is useful for preparing recombinant skin using the organotypic epithelial raft culture system. The sheets of epithelial cells is composed of a stratified sqaumous epithelium composed of a lower layer of immature basal cells and an upper layer of mature keratinized epithelium. The rAAV virus stock prepared without wild type AAV is useful in expressing heterologous protein, however, the maximum production of the heterologous protein was achieved; when the rAAV virus stock contained wild type AAV. This invention discloses that AAV is appropriate for genetically altering skin to secrete new proteins to treat diseases and skin disorders or conditions.

Abstract: The present invention utilizes AAV as a vector to transfect epithelial cells with an AAV/heterologous gene containing/Neo vector, to form a heterologous protein secreting culture of epithelial cells. This culture is useful for preparing recombinant skin using the organotypic epithelial raft culture system. The sheets of epithelial cells is composed of a stratified sqaumous epithelium composed of a lower layer of immature basal cells and an upper layer of mature keratinized epithelium. The rAAV virus stock prepared without wild type AAV is useful in expressing heterologous protein, however, the maximum production of the heterologous protein was achieved; when the rAAV virus stock contained wild type AAV. This invention discloses that AAV is appropriate for genetically altering skin to secrete new proteins to treat diseases and skin disorders or conditions.

Abstract: The present invention utilizes AAV as a vector to transfect epithelial cells with an AAV/heterologous gene containing/Neo vector, to form a heterologous protein secreting culture of epithelial cells. This culture is useful for preparing recombinant skin using the organotypic epithelial raft culture system. The sheets of epithelial cells is composed of a stratified sqaumous epithelium composed of a lower layer of immature basal cells and an upper layer of mature keratinized epithelium. The rAAV virus stock prepared without wild type AAV is useful in expressing heterologous protein, however, the maximum production of the heterologous protein was achieved; when the rAAV virus stock contained wild type AAV. This invention discloses that AAV is appropriate for genetically altering skin to secrete new proteins to treat diseases and skin disorders or conditions.