Abstract: The present invention provides a method of treating an ocular disease in a subject. In a first step, a nucleic acid is introduced into cells or a tissue. The nucleic acid is introduced by electron avalanche transfection. With this technique, a high electric field induces a vapor bubble and plasma discharge between an electrode and the surrounding medium. The formation of a vapor bubble generates mechanical stress. Plasma discharge through the ionized vapor in the bubble enables connectivity between the electrode and the surrounding medium, so that mechanical stress and electric field are applied simultaneously, which results in permeabilization of the cells or tissue. This permeabilization in turn allows the nucleic acid to enter the cell or tissue. Cells or tissue containing the nucleic acid are then transplanted into an ocular region of the subject.

Abstract: A peer-to-peer commerce system in part relies on being able to identify the geographic location of the electronic devices, e.g., computers or palm pilots. Where the computer or device is stationary, this can be indicated by inserting a zip code, address or telephone number in the computer code identifying the device. Where the device is mobile, this can be indicated manually in a similar fashion. In addition, global positioning satellite (“GSP”) devices may be incorporated with the device to give geographic location automatically. Further, if the device is a cellular telephone device, the cellular device has location information in terms of identifying the cell in which the device is broadcasting. The peer-to-peer search is passed simultaneously over various branches of a binary tree to applications controlling databases for various businesses. Each business application checks the query against the information on its database and provides it to the requesting computer when available.