Abstract: A fluid delivery system including a filter for filtering solid particles from a liquid sample, the filter including a first filter member configured to filter solid media from a liquid sample, a second filter member fluidly connected to the first filter member, and a third filter member fluidly connected to a downstream end of the second filter member. The second filter member is configured to filter relatively smaller solid media from the liquid sample than the first filter member, and the third filter member is configured to filter relatively larger solid media from the liquid sample than the second filter member. In various aspects, the system includes a displacement-type plunger needle to draw sample fluid from a vial, a cantilevered carousel for rotatably supporting an array of sample holding vials, and a support for supporting the carousel. Method of using the fluid delivery system are also disclosed.

Abstract: A high-pressure capillary degas assembly includes an assembly block having an eluent inlet and an eluent outlet, and a separator support extending therebetween, a wash inlet and a wash outlet and a groove extending therebetween, a tubular separator extending within the assembly block, the separator having an inner surface and an outer surface, the inner surface defining an eluent fluid line fluidly interconnecting the eluent inlet and outlet, the outer surface abutting against the separator support and enclosing the groove to define a wash line within the groove. The separator may be configured to retain liquid in the eluent fluid line at a first pressure and allow gas to flow through the separator to the wash fluid line at a second pressure lower than the first pressure. A method of using the high-pressure capillary degas assembly is also disclosed.

Abstract: A degas assembly including a low pressure fluid channel for carrying a wash fluid at a first pressure, a pressurized channel for carrying eluent including a gas at a second pressure higher than the first pressure, and a degas separator defining a fluid barrier between the low pressure fluid channel and pressurized fluid channel, the separator configured to retain liquid in the pressurized fluid channel and allow gas to flow through the separator to the low pressure fluid channel. The pressurized fluid channel may extend along an outer periphery of the low pressure fluid channel. The eluent may be received from an eluent generator at a pressure of at least about 3300 psi, and in various embodiments up to about 5000 psi. A liquid chromatography system and method are also disclosed.

Abstract: A high-pressure capillary degas assembly includes an assembly block having an eluent inlet and an eluent outlet, and a separator support extending therebetween, a wash inlet and a wash outlet and a groove extending therebetween, a tubular separator extending within the assembly block, the separator having an inner surface and an outer surface, the inner surface defining an eluent fluid line fluidly interconnecting the eluent inlet and outlet, the outer surface abutting against the separator support and enclosing the groove to define a wash line within the groove. The separator may be configured to retain liquid in the eluent fluid line at a first pressure and allow gas to flow through the separator to the wash fluid line at a second pressure lower than the first pressure. A method of using the high-pressure capillary degas assembly is also disclosed.

Abstract: A degas assembly including a low pressure fluid channel for carrying a wash fluid at a first pressure, a pressurized channel for carrying eluent including a gas at a second pressure higher than the first pressure, and a degas separator defining a fluid barrier between the low pressure fluid channel and pressurized fluid channel, the separator configured to retain liquid in the pressurized fluid channel and allow gas to flow through the separator to the low pressure fluid channel. The pressurized fluid channel may extend along an outer periphery of the low pressure fluid channel. The eluent may be received from an eluent generator at a pressure of at least about 3300 psi, and in various embodiments up to about 5000 psi. A liquid chromatography system and method are also disclosed.

Abstract: A fluid delivery system including a filter for filtering solid particles from a liquid sample, the filter including a first filter member configured to filter solid media from a liquid sample, a second filter member fluidly connected to the first filter member, and a third filter member fluidly connected to a downstream end of the second filter member. The second filter member is configured to filter relatively smaller solid media from the liquid sample than the first filter member, and the third filter member is configured to filter relatively larger solid media from the liquid sample than the second filter member. In various aspects, the system includes a displacement-type plunger needle to draw sample fluid from a vial, a cantilevered carousel for rotatably supporting an array of sample holding vials, and a support for supporting the carousel. Method of using the fluid delivery system are also disclosed.