Abstract: The presently described techniques provide for the use of low-pass sequencing data in the calculation of a polygenic risk score for an individual. As discussed herein, the low-pass sequencing data may be acquired in a context where DNA (e.g., cfDNA) from more than one source is present in the sample and the portion of the DNA attributable to a secondary source may bias the PRS calculation for the primary individual of interest. In one implementation fragment length may be used to derive a function (e.g., a linear function) relating fetal fraction to the respective PRS estimate at each fetal fraction. This function may then be used to calculate the PRS in the absence of a fetal contribution (i.e., at a 0% fetal fraction).

Abstract: Presented are automated fluid handling systems and automated sequencing methods for re-analyzing a sample to achieve a more informative test result. In one embodiment, a method of processing a sample nucleic acid to identify a target mutation comprises performing a first sequencing reaction to determine sample specific properties. The method further comprises determining a statistical measure to determine if a first read coverage for the target mutation from the first sequencing reaction is above or below a threshold. If the determined first read coverage does not exceed the threshold, the method further comprises determining if a sufficient amount of sample nucleic acid is available to perform a second sequencing reaction to increase the read coverage above the threshold. If a sufficient amount of sample nucleic acid is available, the method proceeds to perform re-sequencing of the sample nucleic acid to achieve a second read coverage exceeding the threshold.

Abstract: An imaging member with a unique overcoat composition adapted for forming protective, scratch-resistant overcoats is disclosed. The overcoat composition comprises a binder component that includes particular proportions of a acrylated polyol and propylene glycol. The overcoat composition also comprises a transport agent and a crosslinking agent.

Abstract: An electrophotographic imaging member includes a substrate, a charge generating layer, a charge transport layer, and an overcoating layer, the overcoating layer including a cured polyester polyol or cured acrylated polyol film forming resin and a charge transport material.