Abstract: The present invention relates to the field of oligonucleotide conjugates and to methods of synthesis thereof. In the present method a low-water content solvent environment allows a more efficient conjugation, reducing the amount of conjugate moiety needed and increasing the conjugation reaction speed.

Abstract: Methods for cleaving oligonucleotides from a solid support are described as are methods for synthesizing an oligonucleotide on a solid support and subsequently cleaving the oligonucleotide from the solid support. In the methods, the 3? nucleoside of the oligonucleotide attached to the solid support is a LNA nucleoside. The method entail treating the bound oligonucleotide with a concentrated ammonium hydroxide solution for about 30 minutes to about 6 hours.

Abstract: The present invention relates to a method for determining the amount of an oligonucleotide present in a powdered form. The method does not require the experimental determination of the extinction coefficient of the oligonucleotide or the serial dilution and A260 measurement. The method is, for example, applicable to modified oligonucleotides including 2? sugar-modified oligonucleotides, phosphorothioate oligonucleotides, reporter labelled oligonucleotides, and conjugated oligonucleotides.

Abstract: The present invention relates to the field of oligonucleotide synthesis using a solid support and in particular to the finding the when using a 3? oxy-LNA nucleoside attached to the solid support of the UnyLinker types, cleavage and deprotection occurs very rapidly.

Abstract: The present invention relates to the field of oligonucleotide conjugates and to methods of synthesis thereof. In the present method a low-water content solvent environment allows a more efficient conjugation, reducing the amount of conjugate moiety needed and increasing the conjugation reaction speed.

Abstract: The present invention relates to the field of oligonucleotide conjugates and to methods of synthesis thereof. In the present method a low-water content solvent environment allows a more efficient conjugation, reducing the amount of conjugate moiety needed and increasing the conjugation reaction speed.

Abstract: The present invention relates to the field of oligonucleotide conjugates and to methods of synthesis thereof. In the present method a low-water content solvent environment allows a more efficient conjugation, reducing the amount of conjugate moiety needed and increasing the conjugation reaction speed.

Abstract: The present invention relates to a process for preparing a short oligonucleotide comprising the steps of: (i) preparing a crude mixture comprising the oligonucleotide (ii) subjecting the mixture formed in step (i) to a desalting step; wherein the process does not comprise a chromatographic purification step.

Abstract: The present invention provides novel quencher composition comprising anthraquinone quencher moieties. The anthraquinone quencher moieties are useful as quencher labels when attached to biomolecules such as natural or modified polynucleotides, oligonucleotides, nucleosides, nucleotides, carbohydrates and peptides. For example, polynucleotides can be labeled at the 3? terminus with fluorescence quencher solid support compositions, and polynucleotides can be labeled at internally or at the 5? terminus. The detectable probes may have a format like molecular beacons, scorpion probes, sunrise probes, conformationally assisted probes and TaqMan probes.

Abstract: The invention relates to probes which are extendable useful as PCR probes and in probe libraries. The invention further relates to prevention of replication of a primer extension product in PCR reactions.

Abstract: The present invention provides novel quencher composition comprising anthraquinone quencher moieties. The anthraquinone quencher moieties are useful as quencher labels when attached to biomolecules such as natural or modified polynucleotides, oligonucleotides, nucleosides, nucleotides, carbohydrates and peptides. For example, polynucleotides can be labeled at the 3? terminus with fluorescence quencher solid support compositions, and polynucleotides can be labeled at internally or at the 5? terminus. The detectable probes may have a format like molecular beacons, scorpion probes, sunrise probes, conformationally assisted probes and TaqMan probes.