Abstract: This invention relates to methods and compositions for assessing an amount of total cell-free DNA, such as from a transplant subject. The methods and composition provided herein can be used to determine risk of complications following transplantation, including infection, cardiac arrest, and death, in a subject.

Abstract: A smart-home device may include an energy-storage element that stores energy harvested from an environmental system; a power wire connector and a return wire connector; and switching elements configured to operate in a first state where the switching elements create a connection between the power and the return; and a second state where the switching elements interrupt the connection between the power and return. The smart-home device may also include a circuit that controls the switching elements, where the circuit is configured to detect a zero-crossing of a current received through the power wire connector; wait for a first time interval after the zero-crossing is detected; after an expiration of the first time interval, enable active power stealing for a second time interval; and after an expiration of the second time interval, disable active power stealing.

Abstract: This invention relates to methods and compositions for assessing an amount of total cell-free DNA, such as from a subject having a pro-inflammatory response, such as a pro-inflammatory response associated with an infection, such as a COVID-19 infection. This invention also relates to compositions and methods for treating subjects having or suspected of having a pro-inflammatory response, such as a pro-inflammatory response associated with an infection, such as a COVID-19 infection. In embodiments, there is a risk of an inappropriate or detrimental pro-inflammatory response, such as in a subject with a COVID-19 infection. The methods and composition provided herein can be used to determine severity and risk of complications in a subject. The methods and composition provided herein can also be used to assess the effectiveness of a therapy in a subject, such as a COVID-19 subject.

Abstract: A method of powering a controller using an intermediate device with power from an environmental system may include receiving current from a power wire from the environmental system; passing the current from the power wire to a second command wire from the controller; monitoring the current flowing between the power wire and the second command wire while the current is below a threshold indicative of an amount of current used to power the controller from the environmental system; detecting when the current flowing between the power wire and the second command wire exceeds the threshold indicating that the controller is sending a command to the environmental system to perform the function; and sending a command to environmental system using a first command wire from the environmental system after detecting that the current exceeds the threshold.

Abstract: Systems and methods are provided for using automated mobile computing device testing to ensure that a given targeted message is actually appearing in an intended manner for various types of intended recipients. The intended content or format of a target message to be presented by each of a plurality of mobile computing devices may be based on characteristics of each device. The targeted message may be sent to each device, and an indication of the actual content and/or format of the targeted message as presented by each device may be received and compared to the intended content or format of the targeted message for each device. Errors associated with the targeted message may be detected based on any discrepancies between the intended content and/or format of the targeted message for each device and the actual content or format of the targeted message as presented by each device.

Abstract: Systems and methods relating to detection and remediation of underperforming third party processes are disclosed. Such systems and methods include implementing web page performance profiling by loading a target web page in a selected testing environment and acquiring data related to performance metrics of third party processes in the selected testing environment. The techniques may be applied to manage third party embedded processes on a client website and implementing a corrective action to remediate the impacting events before the underperforming third party processes are further accessed, thus improving website performance.

Abstract: A smart-home device may include a plurality of wire connectors configured to receive a plurality of signals from an environmental system; a plurality of diodes configured to receive the plurality of signals from the plurality of wire connectors, wherein at least two of the plurality of diodes have different voltage drops; and a power-stealing circuit that is configured to receive outputs of the plurality of diodes and to steal power through one of the plurality of wire connectors that is determined at least in part by the different voltage drops.

Abstract: A method of powering a controller using an intermediate device with power from an environmental system may include receiving current from a power wire from the environmental system; passing the current from the power wire to a second command wire from the controller; monitoring the current flowing between the power wire and the second command wire while the current is below a threshold indicative of an amount of current used to power the controller from the environmental system; detecting when the current flowing between the power wire and the second command wire exceeds the threshold indicating that the controller is sending a command to the environmental system to perform the function; and sending a command to environmental system using a first command wire from the environmental system after detecting that the current exceeds the threshold.

Abstract: The present invention relates to lipids and compositions thereof. In various aspects of the invention, the compositions are lipid nanoparticle compositions used to deliver various nucleic acid molecules and/or therapeutic agents to selected targets, such as cells for gene delivery, and/or to prevent or treat diseases or disorders in a subject in need thereof.

Abstract: The present invention relates to lipid nanoparticles (LNP) or compositions thereof for delivery of mRNA molecules encoding CAR, nucleic acid molecule, and/or therapeutic agents to selected targets, such as cells. Thus, in various aspects, the present invention also provides methods of preventing or treating diseases or disorders in a subject in need thereof using the said LNPs or compositions thereof.

Abstract: Methods and systems are disclosed for coordinating content selection across devices. A user device may transmit a request for webpage content and in response, may receive response communications that include the webpage content, embedded content, and a script. The user device may retrieve a device identifier initially generated by a remote system from a locally stored data object. The user device can detect, using the script, whether at least part of the embedded content is being presented within a portion of the webpage that is displayed on a screen of the user device. The user device may then transmit a report communication to a remote ID-graph system that includes the device identifier and an indication as to whether the at least part of the embedded content was presented within the portion of the webpage that was displayed on the screen of the user device.

Abstract: Woven irrigation tubing comprises a woven, extrusion coated & laminated tube formed of a high density polyethylene (HDPE) outer layer, a low density polyethylene (LDPE) middle layer and a linear low density polyethylene (LLDPE) inner layer. The finished tubing is treated for ultraviolet resistance. The tubing is tied off at a distal end with a proximal end connected to a pressurized irrigation source. Watering holes are created in the tubing at spaced intervals and the resulting water streams are directed into parallel plowed furrows. The tubing is completely recyclable. The tubing is formed by manufacturing tape for the woven outer tubing cover, stretching the tape along its length to strengthen it, weaving the outer layer from the tape, flattening the woven outer layer, extrusion coating each surface of the outer layer with LDPE, laminating the LLDPE inner layer to the LDPE, reversing and winding the tubing for storage and distribution.

Abstract: This invention relates to methods and compositions for assessing an amount of donor-specific fraction of cell-free DNA, such as from a subject. The methods and compositions provided herein can be used to determine risk of a condition, such as transplant organ injury (e.g., cellular injury), cellular rejection grade, antibody-mediated rejection, cardiac allograft vasculopathy, and/or cardiac arrest in a transplant subject.

Abstract: Woven irrigation tubing comprising a woven, extrusion coated & laminated tube formed of a high density polyethylene (HDPE) outer layer, a low density polyethylene (LDPE) middle layer and a linear low density polyethylene (LLDPE) inner layer. The finished tubing is treated for ultraviolet resistance. The tubing is tied off at a distal end with a proximal end connected to a pressurized irrigation source. Watering holes are created in the tubing at spaced intervals and the resulting water streams are directed into parallel plowed furrows. The tubing is completely recyclable. The tubing is formed by manufacturing tape for the woven outer tubing cover, stretching the tape along its length to strengthen it, weaving the outer layer from the tape, flattening the woven outer layer, extrusion coating each surface of the outer layer with LDPE, laminating the LLDPE inner layer to the LDPE, reversing and winding the tubing for storage and distribution.

Abstract: A suitability analysis platform may receive one or more environmental parameter values of one or more environmental parameters collected for a plurality of sites by multiple sensor devices. The platform may aggregate the one or more environmental parameter values collected for each site of the plurality of sites by each site. Subsequently, the platform may receive queries for suitable sites for performing activities from user devices, in which each of the queries includes one or more environmental parameter value criteria for at least one corresponding environmental parameter of a suitable site for performing a specific activity. The platform may provide responses to the queries in which each response includes information on one or more sites of the plurality of sites with one or more environmental parameter values that match or are closest to the one or more environmental parameter value criteria of the corresponding query.

Abstract: This invention relates to methods and compositions for assessing risk by obtaining the product of a donor-specific fraction and total cell-free DNA, such as from a transplant subject. The methods and compositions provided herein can be used to assess a transplant subject to determine risk of one or more transplant complications (e.g., death) and/or a need for monitoring or treatment.

Abstract: Systems and methods for monitoring mobile devices in a controlled environment are provided. One or more mobile devices may be accessible through a communication port of the mobile device. One or more monitoring devices may be configured to monitor the one or more mobile devices. A processor in communication with the one or more monitoring devices may control the monitoring devices and receive signals from the monitoring devices. A server in communication with the mobile devices may create a communication channel with each of the mobile devices, with each communication channel accessible through a network for use by software developers and testers. Thus, monitoring and detection of malfunctions or other abnormalities of the mobile devices is achieved, and remediation may be performed.

Abstract: A method of powering a controller using an intermediate device with power from an environmental system may include receiving current from a power wire from the environmental system; passing the current from the power wire to a second command wire from the controller; monitoring the current flowing between the power wire and the second command wire while the current is below a threshold indicative of an amount of current used to power the controller from the environmental system; detecting when the current flowing between the power wire and the second command wire exceeds the threshold indicating that the controller is sending a command to the environmental system to perform the function; and sending a command to environmental system using a first command wire from the environmental system after detecting that the current exceeds the threshold.

Abstract: Adaptive automated testing methods and systems are disclosed. In an embodiment, an automatic test controller of an automated test system may collect a log of a plurality of actions executed by a plurality of devices of the automated test system and apply a heuristic to the log as the automated test system operates to determine whether to execute a next action by a device of the automated test system. When the next action is to be executed, the automatic test controller automatically directs the device to execute the next action. Otherwise, the automatic test controller indicates the next action was intentionally skipped by recording such omission in the log and automatically skipping the next action.

Abstract: This invention relates to methods and compositions for assessing amount(s) of total cell-free DNA in a subject. Such amount(s) can be used to determine risk in a subject, which subjects are non-transplant subjects, such as surgical subjects, in some preferred embodiments.