Abstract: A computer-implemented method of predicting tire pressure of a tire of an aircraft, the method including obtaining first data indicative of an inflation pressure of the tire, obtaining second data indicative of a brake temperature of a brake associated with an aircraft wheel to which the tire is mounted, obtaining third data indicative of a gas temperature of the tire, and obtaining fourth data indicative of one or more predicted operational conditions of the aircraft during a future time period. The method includes determining, based at least in part on the first, second, third and fourth data, predicted tire pressure characteristics of the tire during the future time period.

Abstract: The present invention relates generally to a method for the selection of a modified nucleic acid molecule and to agents useful for same. More particularly, the present invention relates to a method for counterselecting nucleic acid molecules which have undergone targeted modification. The method of the present invention is useful, inter alia, for rapidly and accurately selecting correctly modified nucleic acid molecules, such as modified bacterial artificial chromosomes. The present invention is also directed to counterselection cassettes for use in the method of the invention and to modified nucleic acid molecules selected thereby.