Patents by Inventor Michael P. Kladde

Michael P. Kladde has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Determination of methylation state and chromatin structure of target genetic loci
    Patent number: 10435740
    Abstract: The subject invention pertains to a method of determining methylation state and chromatin structure of target loci. The method comprises treating the genetic material obtained from the cells with DNA methyltransferase, capturing target genetic loci using a set of oligonucleotides, ligating the target loci with oligonucleotide patches that flank the target loci, treating the target loci flanked by oligonucleotide patches with bisulfite, optionally amplifying the target loci by polymerase chain reaction, sequencing the PCR products, and analyzing the sequences to determine methylation state and chromatin structure of the target loci. The current invention also provides a method to identify genes associated with a disease. The invention also provides a method to detect cells suffering from a disease in a group of cells. The current invention also provides kits suitable for carrying out the method of determining methylation state and chromatin structure of the target loci.
    Type: Grant
    Filed: April 1, 2014
    Date of Patent: October 8, 2019
    Assignee: University of Florida Research Foundation, Incorporated
    Inventors: Michael P. Kladde, Nancy H. Nabilsi, Carolina E. Pardo
  • Methyltransferase gene and enzyme
    Patent number: 7034116
    Abstract: A novel cytosine-5 DNA methyltransferase, isolated from Chlorella virus NYs-1, and its encoded enzyme are disclosed. The methyltransferase recognizes a GpC dinucleotide in DNA. Methods of using the novel methyltransferase in high resolution chromatin mapping and related techniques are also disclosed.
    Type: Grant
    Filed: October 18, 2002
    Date of Patent: April 25, 2006
    Assignee: The Penn State Research Foundation
    Inventors: Michael P. Kladde, Robert T. Simpson, Mai Xu
  • Methyltransferase gene and enzyme
    Publication number: 20030073197
    Abstract: A novel cytosine-5 DNA methyltransferase, isolated from Chlorella virus NYs-1, and its encoded enzyme are disclosed. The methyltransferase recognizes a GpC dinucleotide in DNA. Methods of using the novel methyltransferase in high resolution chromatin mapping and related techniques are also disclosed.
    Type: Application
    Filed: October 18, 2002
    Publication date: April 17, 2003
    Applicant: The Penn State Research Foundation
    Inventors: Michael P. Kladde, Robert T. Simpson, Mai Xu
  • Methyltransferase gene and enzyme
    Patent number: 6492168
    Abstract: A novel cytosine-5 DNA methyltransferase, isolated from Chlorella virus NYs-1, and its encoded enzyme are disclosed. The methyltransferase recognizes a GpC dinucleotide in DNA. Methods of using the novel methyltransferase in high resolution chromatin mapping and related techniques are also disclosed.
    Type: Grant
    Filed: April 22, 1999
    Date of Patent: December 10, 2002
    Assignee: The Penn State Research Foundation
    Inventors: Michael P. Kladde, Robert T. Simpson, Mai Xu