Patents by Inventor Michael P. Weiner
Michael P. Weiner has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230212271Abstract: The present disclosure provides a method of making a site-specific antibody to a target of interest.Type: ApplicationFiled: September 27, 2022Publication date: July 6, 2023Inventor: Michael P. WEINER
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Patent number: 10982274Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.Type: GrantFiled: January 16, 2019Date of Patent: April 20, 2021Assignee: Roche Molecular Systems, Inc.Inventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steven Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
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Patent number: 10633701Abstract: The present invention relates to systems and methods for sequencing nucleic acids, including sequencing nucleic acids in fluidic droplets. In one set of embodiments, the method employs sequencing by hybridization using droplets such as microfluidic droplets. In some embodiments, droplets are formed which include a target nucleic acid, a nucleic acid probe, and at least one identification element, such as a fluorescent particle. The nucleic acid probes that hybridize to the target nucleic acid are determined, in some instances, by determining the at least one identification element. The nucleic acid probes that hybridize to the target nucleic acid may be used to determine the sequence of the target nucleic acid. In certain instances, the microfluidic droplets are provided with reagents that modify the nucleic acid probe. In some cases, a droplet, such as those described above, is deformed such that the components of the droplets individually pass a target area.Type: GrantFiled: August 7, 2017Date of Patent: April 28, 2020Assignee: President and Fellows of Harvard CollegeInventors: David A. Weitz, Jeremy Agresti, Michael P. Weiner, Adam R. Abate, Tony Hung
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Publication number: 20190203263Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.Type: ApplicationFiled: January 16, 2019Publication date: July 4, 2019Inventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steve Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
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Patent number: 10240192Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.Type: GrantFiled: June 23, 2016Date of Patent: March 26, 2019Assignee: 454 Life Sciences CorporationInventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steven Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
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Patent number: 9909121Abstract: A method having steps of (a) providing nucleic acids having a tag sequence (N1)n(N2)n . . . (Nx)n, wherein N1, N2 and Nx are nucleotides that complement different nucleotides, respectively, wherein n is an integer that can differ for N1, N2 and Nx; (b) detecting the nucleic acids individually and under conditions to distinguish signal intensities for (N1)n sequences having different values for n, (N2)n sequences having different values for n and. (Nx)n sequences having different values for n; and (c) distinguishing the tags based on the signal intensities.Type: GrantFiled: October 23, 2015Date of Patent: March 6, 2018Assignee: ILLUMINA, INC.Inventor: Michael P. Weiner
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Publication number: 20180057875Abstract: The present invention relates to systems and methods for sequencing nucleic acids, including sequencing nucleic acids in fluidic droplets. In one set of embodiments, the method employs sequencing by hybridization using droplets such as microfluidic droplets. In some embodiments, droplets are formed which include a target nucleic acid, a nucleic acid probe, and at least one identification element, such as a fluorescent particle. The nucleic acid probes that hybridize to the target nucleic acid are determined, in some instances, by determining the at least one identification element. The nucleic acid probes that hybridize to the target nucleic acid may be used to determine the sequence of the target nucleic acid. In certain instances, the microfluidic droplets are provided with reagents that modify the nucleic acid probe. In some cases, a droplet, such as those described above, is deformed such that the components of the droplets individually pass a target area.Type: ApplicationFiled: August 7, 2017Publication date: March 1, 2018Inventors: David A. Weitz, Jeremy Agresti, Michael P. Weiner, Adam R. Abate, Tony Hung
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Patent number: 9797010Abstract: The present invention relates to systems and methods for sequencing nucleic acids, including sequencing nucleic acids in fluidic droplets. In one set of embodiments, the method employs sequencing by hybridization using droplets such as microfluidic droplets. In some embodiments, droplets are formed which include a target nucleic acid, a nucleic acid probe, and at least one identification element, such as a fluorescent particle. The nucleic acid probes that hybridize to the target nucleic acid are determined, in some instances, by determining the at least one identification element. The nucleic acid probes that hybridize to the target nucleic acid may be used to determine the sequence of the target nucleic acid. In certain instances, the microfluidic droplets are provided with reagents that modify the nucleic acid probe. In some cases, a droplet, such as those described above, is deformed such that the components of the droplets individually pass a target area.Type: GrantFiled: December 19, 2008Date of Patent: October 24, 2017Assignee: President and Fellows of Harvard CollegeInventors: David A. Weitz, Jeremy Agresti, Michael P. Weiner, Adam R. Abate, Tony Hung
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Publication number: 20160298175Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.Type: ApplicationFiled: June 23, 2016Publication date: October 13, 2016Inventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steven Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
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Publication number: 20160083718Abstract: A method having steps of (a) providing nucleic acids having a tag sequence (N1)n(N2)n . . . (Nx)n, wherein N1, N2 and Nx are nucleotides that complement different nucleotides, respectively, wherein n is an integer that can differ for N1, N2 and Nx; (b) detecting the nucleic acids individually and under conditions to distinguish signal intensities for (N1)n sequences having different values for n, (N2)n sequences having different values for n and. (Nx)n sequences having different values for n; and (c) distinguishing the tags based on the signal intensities.Type: ApplicationFiled: October 23, 2015Publication date: March 24, 2016Applicant: ILLUMINA, INC.Inventor: Michael P. Weiner
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Patent number: 9200274Abstract: A method having steps of (a) providing nucleic acids having a tag sequence (N1)n(N2)n . . . (Nx)n, wherein N1, N2 and Nx are nucleotides that complement different nucleotides, respectively, wherein n is an integer that can differ for N1, N2 and Nx; (b) detecting the nucleic acids individually and under conditions to distinguish signal intensities for (N1)n sequences having different values for n, (N2)n sequences having different values for n and. (Nx)n sequences having different values for n; and (c) distinguishing the tags based on the signal intensities.Type: GrantFiled: November 19, 2012Date of Patent: December 1, 2015Assignee: Illumina, Inc.Inventor: Michael P. Weiner
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Publication number: 20150099672Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.Type: ApplicationFiled: October 20, 2014Publication date: April 9, 2015Inventors: JAN BERKA, YI-JU CHEN, JOHN H. LEAMON, STEVEN LEFKOWITZ, KENTON L. LOHMAN, VINOD B. MAKHIJANI, JONATHAN M. ROTHBERG, GARY J. SARKIS, MAITHREYAN SRINIVASAN, MICHAEL P. WEINER
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Publication number: 20140342921Abstract: A method having steps of (a) providing nucleic acids having a tag sequence (N1)n(N2)n . . . (Nx)n, wherein N1, N2 and Nx are nucleotides that complement different nucleotides, respectively, wherein n is an integer that can differ for N1, N2 and Nx; (b) detecting the nucleic acids individually and under conditions to distinguish signal intensities for (N1)n sequences having different values for n, (N2)n sequences having different values for n and. (Nx)n sequences having different values for n; and (c) distinguishing the tags based on the signal intensities.Type: ApplicationFiled: November 19, 2012Publication date: November 20, 2014Applicant: ILLUMINA, INC.Inventor: Michael P. Weiner
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Patent number: 8790876Abstract: An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.Type: GrantFiled: April 20, 2007Date of Patent: July 29, 2014Assignee: 454 Life Sciences CorporationInventors: John H. Leamon, Kenton L. Lohman, Jonathan M. Rothberg, Michael P. Weiner
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Patent number: 8765380Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.Type: GrantFiled: September 14, 2012Date of Patent: July 1, 2014Assignee: 454 Life Sciences CorporationInventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steve Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyn Srinivasan, Michael P. Weiner
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Publication number: 20140162885Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.Type: ApplicationFiled: September 25, 2013Publication date: June 12, 2014Applicant: 454 LIFE SCIENCES CORPORATIONInventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steven Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
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Patent number: 8748102Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.Type: GrantFiled: February 23, 2011Date of Patent: June 10, 2014Assignee: 454 Life Sciences CorporationInventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steve Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
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Publication number: 20130078638Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the heads. Also disclosed are kits and apparatuses for performing the methods of the invention.Type: ApplicationFiled: September 14, 2012Publication date: March 28, 2013Inventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steve Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
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Publication number: 20130059741Abstract: This invention provides compositions and methods for assaying the presence of a target analyte in a sample using a solid support. Embodiments of the present invention provide a solid support having a binding protein, such as an antibody, antibody fragment or protein receptor, immobilized to the solid support and at least two separate nucleic acid primers immobilized near the binding protein. This invention also provides a method for tethering two or more polypeptide subunits to generate a multifunctional fusion protein, which can have a primary function, e.g., binding a target analyte, such as a target protein, or an enzymatic activity, and one or more of the subunits of the fusion protein carries out a secondary function, e.g., capture on a solid matrix or quantitation.Type: ApplicationFiled: May 13, 2011Publication date: March 7, 2013Applicant: ILLUMINA, INC.Inventor: Michael P. Weiner
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Publication number: 20120238475Abstract: An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.Type: ApplicationFiled: March 13, 2012Publication date: September 20, 2012Inventors: John H. Leamon, Kenton L. Lohman, Jonathan M. Rothberg, Michael P. Weiner