Abstract: A bioconjugate of an E. coli glucosylated O4 antigen polysaccharide covalently linked to a carrier protein and compositions thereof are provided. Also provided are recombinant host cells for producing the bioconjugate, and methods of producing the bioconjugate using the recombinant host cells. The recombinant host cells contain a nucleic acid encoding a glucosyl transferase capable of modifying the E. coli O4 antigen with glucose branching to produce the glucosylated O4 antigen polysaccharide. Bioconjugates of an E. coli glucosylated O4 antigen polysaccharide described herein can be used alone or in combination with one or more additional E. coli O-antigen polysaccharides to induce antibodies against an E. coli glucosylated antigen, and to vaccinate a subject against extra-intestinal pathogenic E. coli (ExPEC).

Abstract: A method and a system for the at least partially automated parking of a motor vehicle, wherein a trajectory is provided, the motor vehicle is driven in an at least partially automated manner along the trajectory, and the trajectory is displayed on a display device with an overview map of an environment. Furthermore, by at least one camera, environment regions of the environment are captured as respective image data, at least some of which are saved allocated to the related environment regions, wherein at the time of display of the overview map, all of the image data saved up until this point in time are displayed in corresponding display regions on the overview map.

Abstract: A method for operating a drive train, having a main and, an auxiliary drive and a speed modulation gearbox with a fixed mechanical transmission ratio between the two drives, for starting and towing a drive train to a defined set rotational speed. The main drive is started via a direct-on-line-start with direct coupling to a supply network. The auxiliary drive is started simultaneously with at a time before or at a time after the main drive. For towing the drive train to the defined set rotational speed, which corresponds to a defined percentage of a rated rotational speed of the drive train, the main drive is operated motorically in forward mode at least at times and accelerated to its rated rotational speed, wherein parallel to this the auxiliary drive is operated motorically in reverse mode at least at times.

Abstract: A method for operating a drive train, having a main and, an auxiliary drive and a speed modulation gearbox with a fixed mechanical transmission ratio between the two drives, for starting and towing a drive train to a defined set rotational speed. The main drive is started via a direct-on-line-start with direct coupling to a supply network. The auxiliary drive is started simultaneously with at a time before or at a time after the main drive. For towing the drive train to the defined set rotational speed, which corresponds to a defined percentage of a rated rotational speed of the drive train, the main drive is operated motorically in forward mode at least at times and accelerated to its rated rotational speed, wherein parallel to this the auxiliary drive is operated motorically in reverse mode at least at times.