Abstract: Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

Abstract: A driver authentication and safety system and method for monitoring and controlling vehicle usage by high-risk drivers. A centralized database comprising a software application can be accessed by an authorized user via a data communications network utilizing a remote computer in order to configure a desired operating profile that matches requirements of the high-risk driver. The operating profile can be loaded to a driver identification and data logging device in conjunction with the remote computer. A master control unit receives a unique identification code from the data logging device to authenticate the high-risk driver and to operate the vehicle within the desired operating profile. A slave control unit receives commands from the master control unit and generates a real time alarm signal if the driver violates the preprogrammed operating profile unique to the driver.

Abstract: Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.

Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.

Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

Abstract: Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.

Abstract: A driver authentication and safety system and method for monitoring and controlling vehicle usage by high-risk drivers. A centralized database comprising a software application can be accessed by an authorized user via a data communications network utilizing a remote computer in order to configure a desired operating profile that matches requirements of the high-risk driver. The operating profile can be loaded to a driver identification and data logging device in conjunction with the remote computer. A master control unit receives a unique identification code from the data logging device to authenticate the high-risk driver and to operate the vehicle within the desired operating profile. A slave control unit receives commands from the master control unit and generates a real time alarm signal if the driver violates the preprogrammed operating profile unique to the driver.

Abstract: A telescoping mast for supporting and raising a load may be telescopically extended by a zipper-action chain extension system further comprising a system for the management of one or more cables, such as power or communication cables traversing the length of the mast, and is capable of being locked into a rigid formation along a range of telescopic lengths.

Abstract: Methods, systems and apparatus for supplier collaboration and data accuracy within a materials requirement planning scenario. An external supplier is easily integrated into an inter-enterprise planning engine by generating uniquely modified external supplier input files that only include demand information, inventory coverage, and bills of material data associated with the external supplier. This modified external supplier data is used to integrate the external supplier into the planning engine, and for generating a requirements build that fulfills a build plan input into the planning engine. The planning engine is preferably an online planning engine, such that, the requirements build may be validated by inputting the most currently available supplier input data into the planning engine for generating a new, updated requirements build online substantially in real time for fulfilling the build plan.

Abstract: Methods, systems and apparatus for exhausting surplus inventory within currently existing supplies. Intra-enterprise and inter-enterprise lists of surplus inventories are input into a planning engine along with allocation rules for establishing priorities and preferences of allocating and exhausting parts within existing inventories. The planning engine also receives central parameter file inputs for identifying those parts within surplus inventory that can be allocated to other enterprises. Using an identifier of a requested part, the planning engine then analyzes the lists of surplus inventories for compatible parts, and builds hierarchy allocation plans using selected inputs dependent upon which list the compatible parts are located. These hierarchy allocation plans are output in tables for allowing a user to select a plan that exhausts surplus inventory prior to such inventory being declared as excess.

Abstract: Methods, systems and apparatus for determining a current state of an order book for order request reconciliation. A customer inputs an order request for a desired quantity of items to be shipped to a recipient into the system. A supplier inputs a shipment quantity of any such previously shipped items into an input table, while a recipient inputs into another table a receipt quantity of any such received items. These input shipment and receipt quantities are then transmitted to a reconciliation program of the system for determining an in-transit quantity that is used along with the receipt quantity to generate an updated, current state of the order book. This current state of the order book substantially avoids any over-shipments, under-shipments and unwanted inventory returns. A Materials Requirement Planning component then generates an order book reconciliation output table for viewing by users using the current state of the order book.

Abstract: A telescoping mast for supporting and raising a load may be telescopically extended by a zipper-action chain extension system further comprising a system for the management of one or more cables, such as power or communication cables traversing the length of the mast, and is capable of being locked into a rigid formation along a range of telescopic lengths.

Abstract: A method and apparatus for debugging applications on a microkernel without invoking services provided by a particular personality. An application debugger is separated into three distinct pieces consisting of a user interface, debug engine, debug probe (instrumentation server). The user interface supports user inputs and debugger output in a language and platform-independent syntax. The debug engine supports semantics for specific language and target operating environments. The debug probe supports low-level query, access and update of the applications's state. The instrumentation server checks to see if a requested application is in its list of known application tasks. The instrumentation server sets an application into debug mode by either attaching to the application from a list of applications that it has constructed by polling the IBM Microkernel loaders or by having the application launched by a given IBM Microkernel loader.

Abstract: The present invention provides novel processes for the dewatering of a wide variety of solid-liquid matrices, including primary and secondary sludge, which involve the simultaneous application of pressure and heat to the solid-liquid matrices.

Abstract: A data processing system stores and maintains a plurality of security levels for dynamically linked libraries. Upon loading of an application, and upon determination of which dynamically linked libraries are required by the application, the data processing system determines the predefined security level assigned to the application and loads dynamically linked libraries previously encoded with the predefined security level.

Abstract: The present invention provides novel processes for the dewatering of a wide variety of solid-liquid matrices, including primary and secondary sludge, which involve the simultaneous application of pressure and heat to the solid-liquid matrices.

Abstract: Lazy loading of executable library objects reduces operating system overhead and memory commitment requirements by postponing object loading until object references are expected. Initial task loading allocates only the main executable and library objects referenced by that executable. Secondary referenced objects are not allocated. Object references cause page faults for allocated but not loaded pages. Page fault handling causes loading and fixup of executable objects. Page fault handling also determines the next level of object references and allocates memory for the next object level. Shared memory systems allow sharing of executable objects until explicitly referenced. Once referenced, memory fault causes copying and fixup to referencing task memory area.