Abstract: Protein crystallization droplet actuators, systems and methods are provided. According to one embodiment, a droplet actuator for providing an array of crystallization conditions is provided and includes: (a) two or more processing reservoirs; (b) two or more dispensing reservoirs collectively comprising two or more crystallization reagents; and (c) a port for introducing a sample for crystallization analysis. Systems including the droplet actuator, methods of providing an array of crystallization conditions, and methods of identifying crystallization conditions are also provided.

Abstract: Multiwell droplet actuators, systems and methods are provided. According to one embodiment, a substrate is provided and comprises: (a) one or more input ports for introduction of one or more reagents and/or samples; (b) a regular array of processing wells; and (c) a network of droplet transport pathways comprising pathways that provide direct or indirect droplet transport from each of the input ports to each of the one or more processing wells. Varying droplet actuators and systems related thereto are also provided.

Abstract: Protein crystallization screening and optimization droplet actuators, systems and methods are provided. According to one embodiment, a screening droplet actuator is provided and includes: (a) a port for introduction of one or more crystallization reagents and/or one or more protein solutions; and (b) a substrate including: (i) an array of two or more mixing wells; and (ii) electric field mediated microfluidics for moving droplets comprising the crystallization reagents and protein solutions into the mixing wells. Optimization droplet actuators, systems including screening droplet actuators, methods of screening protein crystallization conditions, and methods of testing conditions for growing a crystal are also provided.

Abstract: The present invention relates to a droplet-based nucleic acid amplification device, system, and method. According to one embodiment, a droplet microactuator is provided and includes: (a) a substrate comprising electrodes for conducting droplet operations; and (b) one or more temperature control means arranged in proximity with one or more of the electrodes for heating and/or cooling a region of the droplet microactuator and arranged such that a droplet can be transported on the electrodes into the region for heating.

Abstract: Systems and methods are provided for transferring physical layer control information from a central access point to individual subscriber units while maintaining transparency to higher layers. Adaptation of wireline MAC protocols to wireless applications is greatly facilitated. Subscriber unit power level may be controlled from the central access point.

Abstract: Systems for controlling a droplet microactuator are provided. According to one embodiment, a system is provided and includes a controller, a droplet microactuator electronically coupled to the controller, and a display device displaying a user interface electronically coupled to the controller, wherein the system is programmed and configured to permit a user to effect a droplet manipulation by interacting with the user interface. According to another embodiment, a system is provided and includes a processor, a display device electronically coupled to the processor, and software loaded and/or stored in a storage device electronically coupled to the controller, a memory device electronically coupled to the controller, and/or the controller and programmed to display an interactive map of a droplet microactuator.

Abstract: The present invention relates to droplet-based affinity assays. According to one embodiment, a method of detecting a target analyte in a sample is provided, wherein the method includes: (a) executing droplet operations to combine affinity-based assay reagents on a droplet microactuator with a sample potentially comprising the target analyte to generate a signal indicative of the presence, absence and/or quantity of analyte; and (b) detecting the signal, wherein the signal corresponds to the presence, absence and/or quantity of the analyte in the sample.

Abstract: Methods for amplifying nucleic acid, such as DNA or RNA, on a printed circuit board (PCB) substrate are disclosed. The amplification can be achieved through a variety of methods such as thermocycling or isothermally. The printed circuit board substrate can comprise an array of electrodes for transporting droplets and can be part of a sandwich structure including a top plate parallel to the printed circuit board substrate.

Abstract: A space-time signal processing system with advantageously reduced complexity. The system may take advantage of multiple transmitter antenna elements and/or multiple receiver antenna elements, or multiple polarizations of a single transmitter antenna element and/or single receiver antenna element. The system is not restricted to wireless contexts and may exploit any channel having multiple inputs or multiple outputs and certain other characteristics. Multi-path effects in a transmission medium cause a multiplicative increase in capacity.

Abstract: Apparatuses for mixing droplets, such as a binary mixing apparatus, are provided. The binary mixing apparatus includes an array of electrodes and a conducting element positioned in relation to at least one of the electrodes to enable a droplet placed in electrical communication with the at least one electrode to electrically communicate with the conducting element. The binary mixing apparatus additionally includes an electrode selector for sequentially biasing one or more selected electrodes of the array to move a droplet disposed on the array into contact with another droplet. The apparatus further includes a first droplet supply area communicating with the array and a second droplet supply area communicating with the array.

Abstract: Methods for performing microfluidic sampling are provided. The method includes providing a substrate including an arrangement of first, second and third electrodes, wherein the second electrode is interposed between the first and third electrodes. The method additionally includes causing a fluid input to continuously flow to the first electrode and biasing the first, second and third electrodes to a first voltage to cause a portion of the fluid input to spread across the second and third electrodes. The method further includes biasing the second electrode to a second voltage different from the first voltage to form a droplet on the third electrode, the droplet being separate from the fluid input.

Abstract: A space-time signal processing system with advantageously reduced complexity. The system may take advantage of multiple transmitter antenna elements and/or multiple receiver antenna elements, or multiple polarizations of a single transmitter antenna element and/or single receiver antenna element. The system is not restricted to wireless contexts and may exploit any channel having multiple inputs or multiple outputs and certain other characteristics. Multi-path effects in a transmission medium cause a multiplicative increase in capacity.

Abstract: Apparatuses and methods for manipulating droplets on a printed circuit board (PCB) are disclosed. Droplets are actuated upon a printed circuit board substrate surface by the application of electrical potentials to electrodes defined on the PCB. The use of soldermask as an electrode insulator for droplet manipulation as well techniques for adapting other traditional PCB layers and materials for droplet-based microfluidics are also disclosed.

Abstract: Systems and methods for controlling subscriber unit power level using power measurements made on access request transmissions. Since a subscriber unit access request typically precedes a subscriber unit data transmission by a relatively short period of time, the data transmission power level will then be based on a recent power measurement. The access request may also trigger a sequence of power control steps including the transmission of special upstream power measurement transmissions which can be used as the basis for even more accurate power control. These power control features are particularly useful in fading environments such as found in a wireless system.

Abstract: Improved adjustment of transmission power in a communication system is provided. In one embodiment, in a point to multipoint communication system, transmission power of a subscriber unit is controlled based on power measurements made at a central access point. Power measurement information based on transmissions occurring at irregular intervals may be combined in a beneficial manner to control transmission output power. In one embodiment, a power regulation process determines a series of difference values indicating the differences between desired received power level at the central access point and measured received power level. A smoothing process is applied to the difference values. One or more parameters of the smoothing process vary with elapsed time since a last available power measurement.

Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.

Abstract: A medium access contention protocol that is highly beneficial in wireless networks and particularly in wireless networks that employ a fixed minimum burst size such as OFDM wireless networks. In one embodiment, a MAC protocol is a demand-assigned protocol that maximizes utilization of the bus medium (the allocated frequency spectrum.) Each data communication device (DCD) in the network communicates with a central access point (AP). Multiple DCDs may request access from the AP in the same request access (RA) burst. Each of the multiple DCDs transmits its access request to the AP within a frequency domain channel in the RA burst that is orthogonal to the frequency domain channels used by the other DCDs requesting access. Each DCD includes channel training information in the access request burst to allow the AP and/or DCD to adapt to rapid variations in channel characteristics.

Abstract: A space-time signal processing system with advantageously reduced complexity. The system may take advantage of multiple transmitter antenna elements and/or multiple receiver antenna elements, or multiple polarizations of a single transmitter antenna element and/or single receiver antenna element. The system is not restricted to wireless contexts and may exploit any channel having multiple inputs or multiple outputs and certain other characteristics. Multi-path effects in a transmission medium cause a multiplicative increase in capacity.

Abstract: Systems and methods for efficiently transmitting power level information in a point to multipoint wireless system. Multiple subscriber units may transmit indications of their power level within a shared burst where one or more subchannels are allocated to each subscriber unit. The burst holding the power level information may be understood by the MAC layer to be a data burst. In this way, a wireline MAC protocol may be applied to wireless applications.

Abstract: A space-time signal processing system with advantageously reduced complexity. The system may take advantage of multiple transmitter antenna elements and/or multiple receiver antenna elements, or multiple polarizations of a single transmitter antenna element and/or single receiver antenna element. The system is not restricted to wireless contexts and may exploit any channel having multiple inputs or multiple outputs and certain other characteristics. Multi-path effects in a transmission medium cause a multiplicative increase in capacity.