Abstract: A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.

Abstract: Aspects of the present invention provide a corn protein isolate, comprising at least about 85 wt % corn protein on a dry basis; an “a*” color value ranging from about ?0.5 and 1.5, and a “b” color value ranging from about 10 and 25; and less than about 1.5% oil on a dry basis. Further aspects include methods of making the same.

Abstract: A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.

Abstract: The present invention provides a protective coating for protecting a substrate from deleterious elements present in environments in which the substrates are deployed and methods and apparatus for deploying a PVC encasement with a longitudinal snap jacket of suitable length and girth to coat a pylon substrate or building girder and provide a filler within the snap jacket and around an encased pylon.

Abstract: A method for preparation of an acidic stabilized syrup concentrate containing rebaudioside B and a stabilizer additive. Acidic stabilized syrup concentrate compositions are also provided.

Abstract: An automation apparatus for controlling irrigation and garden lighting, the apparatus comprising: one or more Switch Modules that are adapted to activate and deactivate one or more managed devices; at least one Control Module coupled to each of the one or more Switch Modules for communicating command data that causes a predetermined Switch Module to activate or deactivate a predetermined respective one or more managed devices; and a Power Supply Module coupled to power cable for providing a source of electrical power to each of the Control Module and the one or more Switch Modules. The apparatus can further include one or more Sensor Modules, the Sensor Module being adapted to obtain sensor data from one or more sensor devices and communicate the sensor data to at least one Control Module. Data communication between the Control Module and Switch Modules or Sensor Modules can be bi-directional and transported across the power cable.

Abstract: A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.

Abstract: The present disclosure relates to protein-starch compositions. The disclosure also relates to processes for preparing the protein-starch compositions. Further, the disclosure relates to uses of the protein-starch compositions in the preparation of adhesives or binders. Further, the disclosure relates to adhesive formulations that include protein-starch containing compositions and to paper products resulting from the processes herein.

Abstract: An inspection apparatus for inspecting high voltage insulators is disclosed. The inspection apparatus includes a first platform having first and second linkages, at least one outer gripping mechanism having first and second arms extending outwardly from the first platform, a second slidable platform adapted to slide along the first and second linkages, and at least one inner gripping mechanism having third and fourth arms extending outwardly from the second slidable platform. The outer and inner gripping mechanisms are adapted to move between an open position where the insulator is received by the outer and inner gripping mechanisms and a closed position where the outer and inner gripping mechanisms engage the insulator.

Abstract: A protection circuit provides transient protection to a solid state circuit of a line interface for wired telecommunications media at a voltage level below the power supply of the solid state circuit and prevents transient currents from entering the power supply distribution where they might cause damage to the protected interface and other circuits. The protection circuit uses a MOSFET, the gate of which is provided with a bias derived from a power supply of the solid state circuit being protected. The bias causes the MOSFET to be always on such that the MOSFET provides an immediate and high transconductance path to ground for transient currents from the line interface.

Abstract: A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.

Abstract: A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.

Abstract: Methods and systems for increasing the production of monatin in a multi-step equilibrium pathway are described. Tryptophan and pyruvate are added to a bioreactor to form a mixture comprising monatin and a plurality of intermediates via a multi-step equilibrium pathway. The methods and systems include operating the bioreactor such that a temperature of the mixture in the bioreactor is less than 25 degrees Celsius, resulting in an increased production of monatin. In some embodiments, the temperature of the mixture in the bioreactor is between about 5 degrees Celsius and about 23 degrees Celsius; in other embodiments, the temperature is between about 10 degrees Celsius and about 18 degrees Celsius.

Abstract: Methods and systems for separating and purifying monatin are described. In the production of monatin, a mixture is formed which includes monatin, starting materials and intermediates. The methods and systems include a membrane having a zeta potential of from about ?19 to ?6 such that the membrane rejects greater than about 90% of the monatin. In some embodiments, the membrane is a nanofiltration membrane.

Abstract: A filtration system for a particulate-laden stream of gas utilizes a group of fabric bags within the upper chamber of a housing as the means by which particulate matter is filtered out of the gas. The gas inlet into a lower chamber of the housing is provided with an inlet guide that directs the incoming gas against the lower side of a porous diffuser located below the group of bags and spanning at least most of the separation zone defined by the bags. Passages through the diffuser are configured to present an array of nozzles so that the gas is subjected to a pressure drop across the diffuser before flowing to the bags. The nozzle effect causes the particulate-laden gas to spread out evenly on the lower, upstream side of the diffuser and pass through the diffuser at substantially equal velocity at all passages, thereby smoothing the flow and distributing it evenly to all bags.

Abstract: A real time Taq-Man PCR assay for detecting multiple serotypes of human papillomavirus (HPV) wherein the number of serotypes detected exceeds the number of colorimetric channels for detection. A biological sample is combined with three oligonucleotide primer/probe sets such that the probes and primers anneal to a target sequence. Each primer/probe set is at least preferential for a specific serotype of an organism. The first and second primer/probe sets are degenerate with respect to each other. The third primer/probe set is not degenerate with respect to the first and second primer/probe sets and discriminates for a third serotype. The third primer/probe set has a signal moiety that emits signal at a wavelength that is the same or different from the wavelength emitted by the signal moiety of the degenerate primer/probe set probes. The target sequences, if present, are amplified and detected.

Abstract: The present disclosure relates to protein-starch compositions. The disclosure also relates to processes for preparing the protein-starch compositions. Further, the disclosure relates to uses of the protein-starch compositions in the preparation of adhesives or binders. Further, the disclosure relates to adhesive formulations that include protein-starch containing compositions and to paper products resulting from the processes herein.

Abstract: A direct chemical lysis composition includes an assay compatible buffer composition and an assay compatible surfactant. When combined with a specimen storage composition, such compositions prevent undesired modifications to nucleic acid and proteins lysed from cells in the biological sample. Assays of samples from such compositions do not require expensive and time-consuming steps such as centrifugation and prolonged high temperature processing. The direct chemical lysis composition of the present invention permits direct nucleic acid extraction from the cells in the biological sample without the need to decant off the transport media or otherwise exchange the transport media with assay compatible buffers. There is no need to combine the sample with proteinase K or another enzyme to extract nucleic acids from the cells. A method for lysing cells to obtain target nucleic acid for assay and a kit for combining the direct chemical lysis composition with a sample are also contemplated.

Abstract: A protection circuit provides transient protection to a solid state circuit of a line interface for wired telecommunications media at a voltage level below the power supply of the solid state circuit and prevents transient currents from entering the power supply distribution where they might cause damage to the protected interface and other circuits. The protection circuit uses a MOSFET, the gate of which is provided with a bias derived from a power supply of the solid state circuit being protected. The bias causes the MOSFET to be always on such that the MOSFET provides an immediate and high transconductance path to ground for transient currents from the line interface.

Abstract: A method of closing a vehicular system having a moveable front bow and a moveable rear bow. The method includes moving the front bow forward, releasing tension from the rear bow, moving the front bow to a closed position, and moving the rear bow to a closed position. In another aspect, a method of minimizing buffeting in a vehicle interior compartment from a sunroof. The method includes moving a first end bow of the sunroof to an open position and moving a second end bow of the sunroof away from a seal.