Patents by Inventor Michael R. Emmert-Buck
Michael R. Emmert-Buck has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200041489Abstract: Methods, techniques, and kits are provided herein for purifying cells using molecular targeting, at a cellular or subcellular level. The techniques comprise labeling a biological sample comprising cells with a probe capable of producing a protective barrier. The barrier is deposited onto the surface of the labeled cells or structures, to protect and retain the biological material under the barrier. A micropurification solution is applied to the biological sample, wherein the micropurification solution degrades, digests, or otherwise processes cells not covered by the barrier, allowing isolation of the target cells. In some aspects, a plurality of probes, each specific to a different target, may be used. The techniques may be performed without the need for complex instrumentation involving microscopy.Type: ApplicationFiled: April 6, 2018Publication date: February 6, 2020Inventors: Michael R. EMMERT-BUCK, Michael Anthony TANGREA
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Patent number: 9108035Abstract: Systems and methods are disclosed for directing magnetizable particles comprising therapeutic agents to a target volume, or for guiding magnetizable particles comprising therapeutic agents from a first target volume to a second target volume, at a distance using a magnetic field, to enable the treatment of diseased areas including areas deep inside a patient's body. The methods may be used to diagnose or treat diseased areas within a patient, for example tumors of the lungs, intestines, and liver, and is also useful in enhancing the permeability of solid tumors to chemotherapeutic agents.Type: GrantFiled: November 8, 2013Date of Patent: August 18, 2015Assignees: University of Maryland, College Park, The United States of America as represented by the Secretary, Department of Health and Human Services, National Institutes of HealthInventors: Benjamin Shapiro, Michael R. Emmert-Buck
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Patent number: 8779312Abstract: The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.Type: GrantFiled: November 25, 2009Date of Patent: July 15, 2014Assignees: United States of America/NIH, 20/20 Genesystems, Inc.Inventors: Vladimir Knezevic, Dan-Paul Hartmann, Michael R. Emmert-Buck
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Publication number: 20140066752Abstract: Systems and methods are disclosed for directing magnetizable particles comprising therapeutic agents to a target volume, or for guiding magnetizable particles comprising therapeutic agents from a first target volume to a second target volume, at a distance using a magnetic field, to enable the treatment of diseased areas including areas deep inside a patient's body. The methods may be used to diagnose or treat diseased areas within a patient, for example tumors of the lungs, intestines, and liver, and is also useful in enhancing the permeability of solid tumors to chemotherapeutic agents.Type: ApplicationFiled: November 8, 2013Publication date: March 6, 2014Applicants: The Government of the United States of America as Represented by the Department of Health and Human, UNIVERSITY OF MARYLAND COLLEGE PARKInventors: Benjamin Shapiro, Michael R. Emmert-Buck
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Patent number: 8597715Abstract: A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target).Type: GrantFiled: April 2, 2010Date of Patent: December 3, 2013Assignee: The United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Michael R. Emmert-Buck, Michael Anthony Tangrea, Robert F. Bonner, Rodrigo Chuaqui, Thomas J. Pohida
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Patent number: 8579787Abstract: Systems and methods are disclosed for directing magnetizable particles comprising therapeutic agents to a target volume, or for guiding magnetizable particles comprising therapeutic agents from a first target volume to a second target volume, at a distance using a magnetic field, to enable the treatment of diseased areas including areas deep inside a patient's body. The methods may be used to diagnose or treat diseased areas within a patient, for example tumors of the lungs, intestines, and liver, and is also useful in enhancing the permeability of solid tumors to chemotherapeutic agents.Type: GrantFiled: May 19, 2009Date of Patent: November 12, 2013Assignees: University of Maryland College Park, The United States of America as represented by the Secretary of the Department of Health and Human Services, National Institutes of HealthInventors: Benjamin Shapiro, Michael R. Emmert-Buck
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Patent number: 8460744Abstract: A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.Type: GrantFiled: February 25, 2010Date of Patent: June 11, 2013Assignee: The United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Robert F. Bonner, Thomas J. Pohida, Michael R. Emmert-Buck, Michael Anthony Tangrea, Rodrigo F. Chuaqui
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Patent number: 8283158Abstract: This invention relates to methods and apparati for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array, such as a gel, membrane, tissue biopsy, etc. Such manipulations particularly include assays and nucleic acid amplification protocols.Type: GrantFiled: October 14, 2009Date of Patent: October 9, 2012Assignee: The United States of America, as represented by the Secretary, Department of Health and Human ServicesInventors: Michael R. Emmert-Buck, Rodrigo F. Chuaqui, Michael A. Tangrea
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Publication number: 20110287951Abstract: The disclosure provides methods, systems, and devices for purifying, transferring or manipulating nucleic acids while maintaining the 2D spatial relationship of the nucleic acids as they were present in the original sample having 2D spatial information.Type: ApplicationFiled: January 29, 2010Publication date: November 24, 2011Inventors: Michael R. Emmert-Buck, Michael Armani, Elisabeth Smela, Benjamin Shapiro, Michael A. Tangrea, Jaime Rodriguez-Canales, Rodrigo Chuaqui, John Gillespie
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Patent number: 7838222Abstract: The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.Type: GrantFiled: March 22, 2006Date of Patent: November 23, 2010Assignees: United States of America/ NIH, 20/20 Genesystems, Inc.Inventors: Vladimir Knezevic, Dan-Paul Hartmann, Michael R. Emmert-Buck
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Publication number: 20100216166Abstract: A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.Type: ApplicationFiled: February 25, 2010Publication date: August 26, 2010Inventors: Robert F. Bonner, Thomas J. Pohida, Michael R. Emmert-Buck, Michael Anthony Tangrea, Rodrigo F. Chuaqui
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Publication number: 20100190177Abstract: A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target).Type: ApplicationFiled: April 2, 2010Publication date: July 29, 2010Inventors: Michael R. Emmert-Buck, Michael Anthony Tangrea, Robert F. Bonner, Rodrigo Chuaqui, Thomas J. Pohida
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Publication number: 20100137160Abstract: The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.Type: ApplicationFiled: November 25, 2009Publication date: June 3, 2010Inventors: Vladimir KNEZEVIC, Dan-Paul Hartmann, Michael R. Emmert-Buck
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Patent number: 7709047Abstract: A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target).Type: GrantFiled: July 23, 2003Date of Patent: May 4, 2010Assignee: The United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Michael R. Emmert-Buck, Michael Anthony Tangrea, Robert F. Bonner, Rodrigo Chuaqui, Thomas J. Pohida
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Publication number: 20100105056Abstract: This invention relates to methods and apparati for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array, such as a gel, membrane, tissue biopsy, etc. Such manipulations particularly include assays and nucleic acid amplification protocols.Type: ApplicationFiled: October 14, 2009Publication date: April 29, 2010Inventors: Michael R. Emmert-Buck, Rodrigo F. Chuaqui, Michael A. Tangrea
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Patent number: 7695752Abstract: A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.Type: GrantFiled: August 12, 2005Date of Patent: April 13, 2010Assignee: The Government of the United States of America as represented by the Secretary of the Department of Health and Human ServicesInventors: Robert F. Bonner, Thomas J. Pohida, Michael R. Emmert-Buck, Michael Anthony Tangrea, Rodrigo F. Chuaqui
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Publication number: 20090287036Abstract: Systems and methods are disclosed for directing magnetizable particles comprising therapeutic agents to a target volume, or for guiding magnetizable particles comprising therapeutic agents from a first target volume to a second target volume, at a distance using a magnetic field, to enable the treatment of diseased areas including areas deep inside a patient's body. The methods may be used to diagnose or treat diseased areas within a patient, for example tumors of the lungs, intestines, and liver, and is also useful in enhancing the permeability of solid tumors to chemotherapeutic agents.Type: ApplicationFiled: May 19, 2009Publication date: November 19, 2009Applicants: UNIVERSITY OF MARYLAND, NATIONAL INSTITUTES OF HEALTHInventors: Benjamin Shapiro, Michael R. Emmert-Buck
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Patent number: 7358347Abstract: The invention relates to the discovery of a novel tumor suppressor gene which is associated with multiple endocrine neoplasia type 1. The gene has been designated MEN1 and the gene product is menin. The absence of this protein and associated mutations in the corresponding gene have been identified in individuals suffering from multiple endocrine neoplasia type 1. The identification of this marker for multiple endocrine neoplasia type 1 has diagnostic uses as well as for gene therapy.Type: GrantFiled: March 4, 1998Date of Patent: April 15, 2008Assignee: The United States of America as represented by the Department of Health and Human ServicesInventors: Settara Chandrasekharappa, Siradanahalli Guru, Pachiappan Manickam, Francis S. Collins, Michael R. Emmert-Buck, Larisa V. Debelenko, Irina A. Lubensky, Lance A. Liotta, Sunita K. Agarwal, Allen M. Spiegel, A. Lee Burns, Stephen J. Marx, Zhengping Zhuang
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Patent number: 7226731Abstract: A novel gene, PB39, that is up-regulated, or over-expressed, in prostate cancer has been identified. The gene has been identified by means of its cDNA obtained by reverse transcription of the corresponding mRNA. Microdissection of prostate glands that had been surgically removed from prostate cancer patients revealed a novel up-regulated transcript in an aggressive prostate carcinoma. Differential analysis for the presence of this gene was carried out from the same glands by comparing transcription in microdissected normal prostatic epithelium versus that in microdissected invasive tumor. The transcript was over-expressed in 5 of 10 prostate carcinomas examined. A variant transcript was over-expressed in 4 of 4 prostate carcinomas, and was found in 1 of 4 normal samples.Type: GrantFiled: July 23, 1999Date of Patent: June 5, 2007Assignee: The United States of America, as represented by the Secretary of the Department of Health and Human ServicesInventors: Rodrigo F. Chuaqui, Kristina A. Cole, Lance A. Liotta, Michael R. Emmert-Buck
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Patent number: 7214477Abstract: The present invention involves methods, systems, and devices for analyzing a biological material, such as a cellular or other specimen. The method includes placing the specimen on a substrate having different capture regions, such as contiguous layers, wherein the different capture regions of the substrate contain different identification molecules, and transferring components of the specimen through the capture regions under conditions that allow the components to interact with different identification molecules in the different regions of the substrate. The components of the specimen can be transferred through the different layers (or other regions) of the substrate by capillary action of a solution moving through the cellular specimen or by electrophoresis.Type: GrantFiled: July 26, 2000Date of Patent: May 8, 2007Assignee: The United States of America as represented by the Secretary of the Department of Health and Human ServicesInventor: Michael R. Emmert-Buck