Patents by Inventor Michael R. Gambini
Michael R. Gambini has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9671342Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.Type: GrantFiled: May 17, 2007Date of Patent: June 6, 2017Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Michael R. Gambini, Edward J. Lakatos, Anthony L. Cerrone, Eugene F. Young, Susan Atwood Stone
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Publication number: 20160178522Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.Type: ApplicationFiled: January 18, 2016Publication date: June 23, 2016Inventors: Michael R. GAMBINI, Edward J. LAKATOS, Anthony L. CERRONE, Eugene F. YOUNG, John G. ATWOOD
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Publication number: 20160178521Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.Type: ApplicationFiled: January 18, 2016Publication date: June 23, 2016Inventors: Michael R. GAMBINI, Edward J. LAKATOS, Anthony L. CERRONE, Eugene F. YOUNG, John G. ATWOOD
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Patent number: 9273353Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.Type: GrantFiled: June 4, 2015Date of Patent: March 1, 2016Assignee: Life Technologies CorporationInventors: Michael R. Gambini, John G. Atwood, Anthony L. Cerrone, Edward J. Lakatos, Eugene F. Young
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Publication number: 20150322488Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.Type: ApplicationFiled: June 4, 2015Publication date: November 12, 2015Inventors: Michael R. GAMBINI, John G. ATWOOD, Anthony L. CERRONE, Edward J. LAKATOS, Eugene F. YOUNG
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Publication number: 20150080256Abstract: A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. Luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. The samples may be injected in the wells, and the samples may be injected with buffers and reagents, by an injector. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, a filter, and a camera lens, whereupon a focused image is created by the optics on the charge-coupled device (CCD) camera. The use of a Fresnel field lens, in combination with a collimator and filter, reduces crosstalk between samples below the level attainable by the prior art. Preferred embodiments of the luminescence detecting apparatus and method disclosed include central processing control of all operations, multiple wavelength filter wheel, and robot handling of samples and reagents.Type: ApplicationFiled: October 15, 2014Publication date: March 19, 2015Inventors: Michael R. GAMBINI, Jeff A. Levi, John C. Voyta, John G. Atwood, Bruce E. DeSimas, II, Edward J. Lakatos, Israel Metal, George Sabak, Yongdong Wang
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Patent number: 8921098Abstract: An instrument for monitoring replication of DNA is provided.Type: GrantFiled: April 18, 2006Date of Patent: December 30, 2014Assignee: Applied Biosystems, LLCInventors: Michael R. Gambini, Edward J. Lakatos, Anthony L. Cerrone, Eugene F. Young, Susan Atwood Stone, Judith K. Atwood
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Patent number: 8557566Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.Type: GrantFiled: January 17, 2006Date of Patent: October 15, 2013Assignee: Applied Biosystems, LLCInventors: Michael R. Gambini, Eugene F. Young, Edward J. Lakatos, Anthony L. Cerrone, Susan Atwood Stone, Judith K. Atwood
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Publication number: 20110186739Abstract: Apparatus for scanning large cargo to detect concealed contents include a mobile platform configured to carry and position at least one X-ray or gamma-ray source and at least one detector array at a plurality of positions with respect to a stationary cargo. The detector array may be mounted on a boom moveably affixed to the mobile platform. Multiple measurements of radiation passing through the cargo for various source-detector orientations can be used to compute volumetric images of concealed content within the cargo.Type: ApplicationFiled: February 4, 2011Publication date: August 4, 2011Applicant: L-3 Communications Security and Detection Systems Inc.Inventors: Andrew D. Foland, Richard Franklin Eilbert, Michael R. Gambini, Nicholas Danvers Penrose Gillett, Ronald S. McNabb, Boris Oreper, David Perticone, Vitaliy Ziskin
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Patent number: 7831012Abstract: An apparatus may comprise a frame supporting at least first and second skewed radiation sources and at least first and second radiation detectors. The first and second radiation detectors may be substantially non-contiguous such that a substantial gap exists between the first and second radiation detectors that is free of any radiation detectors. Each of the first and second radiation detectors may also configured and arranged to detect radiation emitted by each of the first and second skewed radiation sources.Type: GrantFiled: February 9, 2007Date of Patent: November 9, 2010Assignee: L-3 Communications Security and Detection Systems Inc.Inventors: Andrew D. Foland, Richard Franklin Eilbert, Michael R. Gambini, Boris Oreper, Nikolay Rolshud
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Publication number: 20100248387Abstract: A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. Luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. The samples may be injected in the wells, and the samples may be injected with buffers and reagents, by an injector. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, a filter, and a camera lens, whereupon a focused image is created by the optics on the charge-coupled device (CCD) camera. The use of a Fresnel field lens, in combination with a collimator and filter, reduces crosstalk between samples below the level attainable by the prior art. Preferred embodiments of the luminescence detecting apparatus and method disclosed include central processing control of all operations, multiple wavelength filter wheel, and robot handling of samples and reagents.Type: ApplicationFiled: March 2, 2010Publication date: September 30, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Michael R. Gambini, John C. Voyta, John Atwood, Susan A. Atwood-Stone, Bruce E. DeSimas, II, Edward Lakatos, Jeff Levi, Israel Metal, George Sabak, Yongdong Wang
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Patent number: 7670848Abstract: In a luminescence detecting apparatus and method for analyzing luminescent samples, luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, an infrared filter, and a camera lens, whereupon a focused image is created by the optics on the camera. The use of an infrared filter suppresses stray IR radiation resulting from plate phosphorescence (which can result in abnormally high backgrounds and/or alteration of the image received by the camera).Type: GrantFiled: October 18, 2005Date of Patent: March 2, 2010Assignee: Applied Biosystems, LLCInventors: Michael R. Gambini, John C. Voyta, John Atwood, Susan A. Atwood-Stone, legal representative, Bruce E. DeSimas, II, Edward Lakatos, Jeff Levi, Israel Metal, George Sabak, Yongdong Wang
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Patent number: 7435602Abstract: Method and system providing calibration of light detected from biological samples with a correction factor including components for each of a plurality of spectrally distinguishable species and/or for each well and/or for each filter.Type: GrantFiled: January 14, 2004Date of Patent: October 14, 2008Assignee: Applied Biosystems Inc.Inventors: Stephen J. Gunstream, Mark F. Oldham, Michael R. Gambini, John C. Voyta
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Patent number: 7183103Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.Type: GrantFiled: February 20, 2003Date of Patent: February 27, 2007Assignee: Applera CorporationInventors: Michael R. Gambini, Susan Atwood Stone, legal representative, Judith K. Atwood, legal representative, Eugene F. Young, Edward J. Lakatos, Anthony L. Cerrone, John G. Atwood, deceased
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Patent number: 7008789Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.Type: GrantFiled: August 9, 2002Date of Patent: March 7, 2006Assignee: Applera CorporationInventors: Michael R. Gambini, Susan Atwood Stone, legal representative, Judith K. Atwood, legal representative, Eugene F. Young, Edward J. Lakatos, Anthony L. Cerrone, John G. Atwood, deceased
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Publication number: 20040259260Abstract: Method and system providing calibration of light detected from biological samples with a correction factor including components for each of a plurality of spectrally distinguishable species and/or for each well and/or for each filter.Type: ApplicationFiled: January 14, 2004Publication date: December 23, 2004Applicant: Applera CorporationInventors: Stephen J. Gunstream, Mark F. Oldham, Michael R. Gambini, John C. Voyta
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Patent number: 6818437Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.Type: GrantFiled: November 29, 2001Date of Patent: November 16, 2004Assignee: Applera CorporationInventors: Michael R. Gambini, John G. Atwood, Eugene F. Young, Edward J. Lakatos, Anthony L. Cerrone
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Publication number: 20030148505Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.Type: ApplicationFiled: February 20, 2003Publication date: August 7, 2003Inventors: Michael R. Gambini, John G. Atwood, Susan Atwood Stone, Judith K. Atwood, Eugene F. Young, Edward J. Lakatos, Anthony L. Cerrone
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Publication number: 20030092194Abstract: A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. Luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. The samples may be injected in the wells, and the samples may be injected with buffers and reagents, by an injector. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, a filter, and a camera lens, whereupon a focused image is created by the optics on the charge-coupled device (CCD) camera. The use of a Fresnel field lens, in combination with a collimator and filter, reduces crosstalk between samples below the level attainable by the prior art. Preferred embodiments of the luminescence detecting apparatus and method disclosed include central processing control of all operations, multiple wavelength filter wheel, and robot handling of samples and reagents.Type: ApplicationFiled: December 20, 2002Publication date: May 15, 2003Inventors: Michael R. Gambini, John C. Voyta, John Atwood, Bruce E. DeSimas, Edward Lakatos, Jeff Levi, Israel Metal, George Sabak, Yongdong Wang
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Patent number: 6518068Abstract: A luminescence detecting apparatus and method for analyzing luminescent samples is disclosed. Luminescent samples are placed in a plurality of sample wells in a tray, and the tray is placed in a visible-light impervious chamber containing a charge coupled device camera. The samples may be injected in the wells, and the samples may be injected with buffers and reagents, by an injector. In the chamber, light from the luminescent samples pass through a collimator, a Fresnel field lens, a filter, and a camera lens, whereupon a focused image is created by the optics on the charge-coupled device (CCD) camera. The use of a Fresnel field lens, in combination with a collimator and filter, reduces crosstalk between samples below the level attainable by the prior art. Preferred embodiments of the luminescence detecting apparatus and method disclosed include central processing control of all operations, multiple wavelength filter wheel, and robot handling of samples and reagents.Type: GrantFiled: July 21, 2000Date of Patent: February 11, 2003Assignee: Tropix, Inc.Inventors: Michael R. Gambini, John C. Voyta, John Atwood, Bruce E. DeSimas, II, Edward Lakatos, Jeff Levi, Israel Metal, George Sabak, Yongdong Wang