Abstract: The present disclosure provides methods of generating skeletal muscle progenitor cells (SMPCs). The present disclosure provides methods of generating immature SMPCs and multinucleated muscle cells, and matured SMPCs and multinucleated muscle cells. The present disclosure provides engraftment methods and treatment methods, involving generating SMPCs and introducing the SMPCs into an individual.

Abstract: An apparatus is disclosed for applying impedance matching gel to an ultrasonic transducer head of an imaging device for application to a patient. The apparatus comprises: a reservoir for containing the impedance matching gel; a heater for heating the impedance matching gel in the reservoir to a predetermined temperature; a conduit for conveying the impedance matching gel from the reservoir to the ultrasonic transducer head; a pumping arrangement for supplying the heated impedance matching gel from the reservoir, via the conduit, to the ultrasonic transducer head for application to the patient; and a low gel sensing system for indicating when the amount of gel in the reservoir drops below a predetermined quantity to prevent overheating of the gel.