Patents by Inventor Michael R. Lieber

Michael R. Lieber has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • NOVEL ARTEMIS/DNA-DEPENDENT PROTEIN KINASE COMPLEX AND METHODS OF USE THEREOF
    Publication number: 20090017010
    Abstract: In the present invention, it is disclosed that Artemis forms a complex with the 469 kDa DNA-dependent protein kinase (DNA-PKcs) in vitro and in vivo in the absence of DNA. The purified Artemis protein alone possesses single-strand specific 5? to 3? exonuclease activity. Upon complex formation, DNA-PKcs phosphorylates Artemis, and Artemis acquires endonucleolytic activity with respect to single-stranded nucleotides, including 5? and 3? overhangs, as well as hairpins. Further, the Artemis:DNA-PKcs complex can open hairpins generated by the RAG complex from a 12/23-substrate pair. Thus, DNA-PKcs regulates Artemis by both phosphorylation and complex formation to permit enzymatic activities that are critical for the hairpin opening step of V(D)J recombination and for all of the 5? and 3? overhang processing in nonhomologous DNA end joining.
    Type: Application
    Filed: June 12, 2008
    Publication date: January 15, 2009
    Applicant: University of Southern California
    Inventors: Michael R. Lieber, Yunmei Ma, Ulrich Pannicke, Klaus Schwarz
  • Novel Artemis/DNA-dependent protein kinase complex and methods of use thereof
    Publication number: 20040005586
    Abstract: In the present invention, it is disclosed that Artemis forms a complex with the 469 kDa DNA-dependent protein kinase (DNA-PKcs) in vitro and in vivo in the absence of DNA. The purified Artemis protein alone possesses single-strand specific 5′ to 3′ exonuclease activity. Upon complex formation, DNA-PKcs phosphorylates Artemis, and Artemis acquires endonucleolytic activity with respect to single-stranded nucleotides, including 5′ and 3′ overhangs, as well as hairpins. Further, the Artemis:DNA-PKcs complex can open hairpins generated by the RAG complex from a 12/23-substrate pair. Thus, DNA-PKcs regulates Artemis by both phosphorylation and complex formation to permit enzymatic activities that are critical for the hairpin opening step of V(D)J recombination and for all of the 5′ and 3′ overhang processing in nonhomologous DNA end joining.
    Type: Application
    Filed: February 5, 2003
    Publication date: January 8, 2004
    Inventors: Michael R. Lieber, Yunmei Ma, Ulrich Pannicke, Klaus Schwarz
  • Mammalian flap-specific endonuclease
    Patent number: 5874283
    Abstract: Compositions comprising human FEN-1(flap) endonucleases, nucleic acids encoding them, and methods for their use are provided.
    Type: Grant
    Filed: May 30, 1995
    Date of Patent: February 23, 1999
    Assignees: John Joseph Harrington, Michael R. Lieber, Chih-Lin Hsieh
    Inventors: John Joseph Harrington, Chih-Lin Hsieh, Michael R. Lieber