Patents by Inventor Michael R Loken
Michael R Loken has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240044904Abstract: A normal set of cells is characterized using flow cytometry. A centroid and radius are defined for a set of clusters in an n-dimensional space corresponding to a normal maturation for a cell lineage in the normal set of cells. A test set of cells is characterized using flow cytometry and the characterization is compared to the defined set of clusters. Support Vector Machine (SVM) subroutines are employed to identify reference populations of interest by generating multidimensional boundary definitions. These boundary definitions may be used to identify reference populations to use in defining or refining a centroid line or a radius or radii defining a set of normal clusters, and to characterize and compare a test set of cells to the defined set of normal clusters.Type: ApplicationFiled: March 20, 2023Publication date: February 8, 2024Inventors: Michael R. Loken, Andrew P. Voigt
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Patent number: 11639936Abstract: A normal set of cells is characterized using flow cytometry. A centroid and radius are defined for a set of clusters in an n-dimensional space corresponding to a normal maturation for a cell lineage in the normal set of cells. A test set of cells is characterized using flow cytometry and the characterization is compared to the defined set of clusters. Support Vector Machine (SVM) subroutines are employed to identify reference populations of interest by generating multidimensional boundary definitions. These boundary definitions may be used to identify reference populations to use in defining or refining a centroid line or a radius or radii defining a set of normal clusters, and to characterize and compare a test set of cells to the defined set of normal clusters.Type: GrantFiled: September 19, 2017Date of Patent: May 2, 2023Assignee: Hematologics, Inc.Inventors: Michael R. Loken, Andrew P. Voigt
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Publication number: 20200158734Abstract: A normal set of cells is characterized using flow cytometry. A centroid and radius are defined for a set of clusters in an n-dimensional space corresponding to a normal maturation for a cell lineage in the normal set of cells. A test set of cells is characterized using flow cytometry and the characterization is compared to the defined set of clusters. Support Vector Machine (SVM) subroutines are employed to identify reference populations of interest by generating multidimensional boundary definitions. These boundary definitions may be used to identify reference populations to use in defining or refining a centroid line or a radius or radii defining a set of normal clusters, and to characterize and compare a test set of cells to the defined set of normal clusters.Type: ApplicationFiled: September 19, 2017Publication date: May 21, 2020Inventors: Michael R. Loken, Andrew P. Voigt
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Publication number: 20180106787Abstract: A system, method, and article for diagnosing a test set of biological cells. For example, in one embodiment a normal set of cells is characterized using flow cytometry. A centroid and radius are defined for a set of clusters in an n-dimensional space corresponding to a normal maturation for a cell lineage in the normal set of cells. A test set of cells is characterized using flow cytometry and the characterization is compared to the set of clusters.Type: ApplicationFiled: December 19, 2017Publication date: April 19, 2018Inventors: Michael R. Loken, Sanjaya N. Joshi
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Patent number: 9880155Abstract: A system, method, and article for diagnosing a test set of biological cells. For example, in one embodiment a normal set of cells is characterized using flow cytometry. A centroid and radius are defined for a set of clusters in an n-dimensional space corresponding to a normal maturation for a cell lineage in the normal set of cells. A test set of cells is characterized using flow cytometry and the characterization is compared to the set of clusters.Type: GrantFiled: December 5, 2013Date of Patent: January 30, 2018Assignee: Hematologics, Inc.Inventors: Michael R. Loken, Sanjaya N. Joshi
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Publication number: 20140221247Abstract: A system, method, and article for diagnosing a test set of biological cells. For example, in one embodiment a normal set of cells is characterized using flow cytometry. A centroid and radius are defined for a set of clusters in an n-dimensional space corresponding to a normal maturation for a cell lineage in the normal set of cells. A test set of cells is characterized using flow cytometry and the characterization is compared to the set of clusters.Type: ApplicationFiled: December 5, 2013Publication date: August 7, 2014Applicant: Hematologics, Inc.Inventors: Michael R. Loken, Sanjaya N. Joshi
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Patent number: 8630833Abstract: A system, method, and article for diagnosing a test set of biological cells. For example, in one embodiment a normal set of cells is characterized using flow cytometry. A centroid and radius are defined for a set of clusters in an n-dimensional space corresponding to a normal maturation for a cell lineage in the normal set of cells. A test set of cells is characterized using flow cytometry and the characterization is compared to the set of clusters.Type: GrantFiled: February 17, 2006Date of Patent: January 14, 2014Assignee: Hematologics, Inc.Inventors: Michael R. Loken, Sanjaya N. Joshi
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Patent number: 7045295Abstract: The present invention provides improved methods for collecting cells for subsequent analysis. More specifically, the invention provides methods for collecting purified cell samples from a flow cytometer onto a solid support on which cell lysis occurs and genetic material is immobilized.Type: GrantFiled: March 30, 2005Date of Patent: May 16, 2006Assignee: HematoLogics, Inc.Inventor: Michael R Loken
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Patent number: 5840580Abstract: One or more population of cells enriched for human hematopoietic stem cells is disclosed. HSC in this population of cells are capable of limited self-renewal and are capable of differentiating into all elements of the hematopoietic system. This population of cells has the phenotype of CD34.sup.+ /CD38.sup.- and more preferably CD34.sup.+ /CD38.sup.- /HLA-DR.sup.+. Cells within this population have been found to express CD13, CD33 and CD71. Hematopoietic stem cells can be used in a number of therapies, including autologous transplantation and in gene therapy.Type: GrantFiled: May 14, 1997Date of Patent: November 24, 1998Assignee: Becton Dickinson and CompanyInventors: Leon W. Terstappen, Michael R. Loken, Shiang Huang, Johanna Olweus, Fridtjof Lund-Johansen
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Patent number: 5224058Abstract: A method for the non-linear, non-logrithmic transformation of one or more parameters of data gathered by means of flow cytometry is disclosed wherein the method seeks to maximize the resolution between populations of interest for each parameter while maintaining the dynamic range of the data recorded.Type: GrantFiled: May 1, 1990Date of Patent: June 29, 1993Assignee: Becton, Dickinson and CompanyInventors: Ronald A. Mickaels, Richard K. Dost, Leon W. M. M. Terstappen, Michael R. Loken
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Patent number: 5137809Abstract: A method and kit for identification of the lineages and stages of hematopoietic cells in a sample wherein the cells are treated with labeled monoclonal antibodies selected to distinguish between each lineage and maturational stages within each lineage.Type: GrantFiled: July 19, 1990Date of Patent: August 11, 1992Assignee: Becton, Dickinson and CompanyInventors: Michael R. Loken, Curt I. Civin, Virendra O. Shah
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Patent number: 5057413Abstract: A method is disclosed for the discrimination between intact and damaged cells in a sample, and more particularly, the method uses a vital nucleic acid dye to selectively stain intact versus damaged cells which then may be counted and sorted by flow cytometry means. The method also may be used in conjunction with fluorescently labelled monoclonal antibodies to simultaneously identify cellular antigens.Type: GrantFiled: June 13, 1988Date of Patent: October 15, 1991Assignee: Becton, Dickinson and CompanyInventors: Leon W. M. M. Terstappen, Michael R. Loken, Virendra O. Shah
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Patent number: 5047321Abstract: A method for multi-parameter analysis of cells in a body fluid sample is described which makes use of a plurality of fluorescence measurements, comprising at least two nucleic acid dyes and at least one fluorescently labelled cell surface marker, and a plurality of light scattering measurements. A kit containing the nucleic acid dyes and cell surface marker also is described.Type: GrantFiled: June 15, 1988Date of Patent: September 10, 1991Assignee: Becton Dickinson & Co.Inventors: Michael R. Loken, Leon W. M. M. Terstappen
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Patent number: 4940668Abstract: In order to produce a more well defined interface between adjacent cell layers in a centrifuged sample of anticoagulated whole blood, a material which will bond one group of cells together is added to the blood sample prior to centrifugation. The bonding material must produce a high strength bond between one group of cells, but not effect the other cell types. The material is added prior to centrifugation of the blood sample.Type: GrantFiled: September 18, 1987Date of Patent: July 10, 1990Assignee: Becton Dickinson and CompanyInventors: Stephen C. Wardlaw, Robert A. Levine, Rodolfo R. Rodriguez, Michael R. Loken
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Patent number: 4885237Abstract: A method is described for the detection of halodeoxyuridines taken up by cells during DNA synthesis by the use of monoclonal antibodies. The described method also allows the simultaneous detection of cell surface receptors.Type: GrantFiled: June 6, 1988Date of Patent: December 5, 1989Assignee: Becton, Dickinson and CompanyInventors: Elizabeth L. Evans, Nick J. Gonchoroff, Philip R. Greipp, David W. Houck, Jerry A. Katzmann, Robert A. Kyle, Michael R. Loken
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Patent number: 4867908Abstract: A method for calibrating an instrument for using that instrument to obtain at least one light-related signal from particles under analysis comprises directing an incident beam of light at calibration particles having one or more known characteristics related to the particles expected to be analyzed. Both a light signal and a noise signal from the calibration particles are detected. A measurement is made of the ratio of the detected light signal to the detected noise signal, and that measurement is reported. The measured ratio is then compared to a predetermined ratio which represents a threshold for minimum instrument performance. This method further includes adjusting, if the predetermined ratio has not been attained, the operation of the instrument, while the calibration particles are within the incident beam of light, until the measured ratio reaches the predetermined ratio whereby the instrument is calibrated for subsequently obtaining the light signal from particles to be analyzed.Type: GrantFiled: June 4, 1987Date of Patent: September 19, 1989Assignee: Becton, Dickinson and CompanyInventors: Diether J. Recktenwald, Rickie S. Kerndt, Michael R. Loken, Chia H. Chen
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Patent number: 4704891Abstract: A method for calibrating an instrument for using that instrument to obtain at least one light-related signal from particles under analysis comprises directing an incident beam of light at calibration particles having one or more known characteristics related to the particles expected to be analyzed. Both a light signal and a noise signal from the calibration particles are detected. A measurement is made of the ratio of the detected light signal to the detected noise signal, and that measurement is reported. The measured ratio is then compared to a predetermined ratio which represents a threshold for minimum instrument performance. This method further includes adjusting, if the predetermined ratio has not been attained, the operation of the instrument, while the calibration particles are within the incident beam of light, until the measured ratio reaches the predetermined ratio whereby the instrument is calibrated for subsequently obtaining the light signal from particles to be analyzed.Type: GrantFiled: August 29, 1986Date of Patent: November 10, 1987Assignee: Becton, Dickinson and CompanyInventors: Diether J. Recktenwald, Rickie S. Kerndt, Michael R. Loken, Chia H. Chen
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Patent number: 4695553Abstract: In order to produce a more well defined interface between adjacent cell layers in a centrifuged sample of anticoagulated whole blood, a material which will bond one group of cells together is added to the blood sample prior to centrifugation. The bonding material must produce a high strength bond between one group of cells, but not effect the other cell types. The material is added prior to centrifugation of the blood sample. An example of a bonding agent is a monoclonal antibody.Type: GrantFiled: November 1, 1985Date of Patent: September 22, 1987Assignee: Becton Dickinson and Co., Inc.Inventors: Stephen C. Wardlaw, Robert A. Levine, Rodolfo R. Rodriguez, Michael R. Loken