Abstract: A concrete block wet cast ready mix composition preferably includes ground recycled glass, river sand, river gravel, cement, soap, water and a water proofing substance. The river gravel preferably includes natural stone, granite, limestone and domite. The cement is preferably an off white cement. The soap is preferably laundry detergent. The water proofing substance is preferably Grace Optec Admix waterproofing. The wet cast composition is preferably mixed in the following manner. The ground recycled glass, the river sand and the river gravel are mixed together to form an aggregate mix. The cement is combined with the aggregate mix to form a cement aggregate mix. Water is added to the cement aggregate mix to form a wet aggregate mix. Finally, the soap and the waterproofing substance are combined with the wet aggregate mix. The wet cast composition is now ready to be poured into concrete block molds or poured as cast concrete.

Abstract: A concrete block wet cast ready mix composition preferably includes ground recycled glass, river sand, river gravel, cement, soap, water and a water proofing substance. The river gravel preferably includes natural stone, granite, limestone and domite. The cement is preferably an off-white cement. The soap is preferably laundry detergent. The water proofing substance is preferably Grace Optec Admix waterproofing. The wet cast composition is preferably mixed in the following manner. The ground recycled glass, the river sand and the river gravel are mixed together to form an aggregate mix. The cement is combined with the aggregate mix to form a cement aggregate mix. Water is added to the cement aggregate mix to form a wet aggregate mix. Finally, the soap and the waterproofing substance are combined with the wet aggregate mix. The wet cast composition is now ready to be poured into concrete block molds or poured as cast concrete.

Abstract: A system for accurately positioning a reaction mixture during amplification of a nucleic acid includes a reaction vessel that contains the reaction mixture, a first heat exchanger, a second heat exchanger and a pump assembly. The reaction vessel can include .a first zone and a second zone. The first heat exchanger is positioned near the first zone, and the second heat exchanger positioned near the second zone. The first heat exchanger adjusts the temperature of the reaction mixture so the reaction mixture is at a first temperature when the reaction mixture is in the first zone. The second heat exchanger adjusts the temperature of the reaction mixture so the reaction mixture is at a second temperature. The pump assembly adjusts the pressure within the reaction vessel to selectively position the reaction mixture relative to the first zone and the second zone during amplification. The system can include a sensor that monitors the position of the reaction mixture within the reaction vessel.

Abstract: The present invention provides methods for purifying nucleic acid molecules, wherein each method includes the steps of: (a) synthesizing nucleic acid molecules in a reaction mixture; (b) contacting the nucleic acid molecules with a proteinase for a period of time sufficient to degrade protein in the reaction mixture; (c) applying the nucleic acid molecules treated in accordance with step (b) to a size-limiting filter so that at least some of the nucleic acid molecules are trapped on the filter; and (d) washing the filter with a phosphate buffer having a pH in the range of from about 5.7 to about 8.5.

Abstract: Interlocking blocks for construction preferably include an in-line block, a corner block, an offset block and end blocks. The in-line block includes a connector extension disposed on one end and a connector cavity formed on the opposite end thereof. The connector cavity is sized to receive the connector extension. First and second retention projections preferably extend from a face of the in-line block. The corner block preferably includes the connector extension formed on one end and the connector cavity formed in a side at the opposite end thereof. The offset block includes the connector extension formed on one side at one end and the connector cavity formed on the opposite side at the opposite end thereof. The end block includes a connector extension or a connector cavity disposed on one end. At least one cavity is formed through each block.

Abstract: A recreational boat construction utilizes a hull formed of a thin gauge metal, such as aluminum, with the hull having an upper extremity to which are fastened unitary superstructure members molded from a plastic resin. The superstructure panels are rigidly fastened to the upper extremity of the hull and to the deck.