Abstract: The invention provides a modified T cell, or an isolated population of immune cells expressing a CXCR3 isoform selected from CXCR3A, CXCR3B, and CXCR3alt, and optionally, further expressing transgenes comprising an artificial T cell receptor, and/or a CXCR3 ligand, for use as a medicament. The invention also provides the methods to obtain said cells, or populations of cells from a plurality of immune cells derived from a human subject. The invention also relates to assessment of CXCR3 splice variants and its ligands CXCL9, CXCL10, and CXCL11 in muscle-invasive bladder cancer (MIBC) patients, to enable patients to be stratified for their predicted response to a chemotherapy drug treatment, or clinical outcome.

Abstract: A nucleic acid construct for targeting and integrating a CD3 zeta-deficient chimeric antigen receptor (CAR) fragment into an endogenous CD3 zeta/CD247 gene of a host genome. Also disclosed is a genetically modified human cell expressing an exogenous nucleic acid sequence encoding a CD3 zeta deficient CAR fragment, integrated in-frame into the endogenous CD3 zeta/CD247 gene for gene fusion, to form a functional CAR including an exogenous CAR fragment fused with an endogenous CD3 zeta domain.

Abstract: The invention relates to a method for determining T cell mediated immunity towards a CRISPR associated protein by contacting said cell preparation obtained from a patient with a CRISPR associated protein or a peptide mix that represents its amino acid sequence, or a cell manipulated to contain a CRISPR protein polypeptide to provide activated T cells. Subsequently, one or more subpopulations of said activated T cells are marked by specific ligand and counted, and a ratio (TREG/TEFF) of activated regulatory T cells to activated effector T cells is used to assess the immune status of the patient with respect to the CRISPR associated protein. The invention further relates to a method for generating a CRISPR associated protein specific Treg population and its use in therapy.

Abstract: A device for accurately guiding and positioning a surgical instrument bearing suture material to a predetermined area within the body for closing an open wound includes an elongated conically shaped guide that is wider at the top and which has two openings in the side wall thereof. The top has two openings that communicate with the openings in the side wall through generally linear passageways that pass through the guide and which allow surgical instruments carrying suture material to pass therethrough. A thin elongated rod extends upwardly from the top wall approximately two inches and includes a handle at the top thereof. Raising the handle above the guide increases the field of view of the surgeon.

Abstract: The invention relates to a method for generation of T cell preparations that are specific for at least one target antigen, comprising the steps of expanding lymphoid cells in vitro in the presence of a target antigen or peptide fragments thereof in an expansion step, isolating cells that secrete interferon gamma and culturing, the cells in the presence of interleukin 2 and interleukin 7 and either an inhibitor of the mTOR Complex 1, or in the presence of an inhibitor of IL-2/IL-2R interaction. The invention further relates to preparations obtained by the method of the invention.

Abstract: A device for accurately guiding and positioning a surgical instrument bearing suture material to a predetermined area within the body for closing an open wound includes an elongated conically shaped guide that is wider at the top and which has two openings in the side wall thereof. The top has two openings that communicate with the openings in the side wall through generally linear passageways that pass through the guide and which allow surgical instruments carrying suture material to pass therethrough. A thin elongated rod extends upwardly from the top wall approximately two inches and includes a handle at the top thereof. Raising the handle above the guide increases the field of view of the surgeon.

Abstract: The invention relates to a method for generation of T cell preparations that are specific for at least one target antigen, comprising the steps of expanding lymphoid cells in vitro in the presence of a target antigen or peptide fragments thereof in an expansion step, isolating cells that secrete interferon gamma and culturing, the cells in the presence of interleukin 2 and interleukin 7 and either an inhibitor of the mTOR Complex 1, or in the presence of an inhibitor of IL-2/IL-2R interaction. The invention further relates to preparations obtained by the method of the invention.

Abstract: The invention relates to an adjustment device for a valve pin on an injection molding machine, and particularly on a multi-cavity machine, having a plurality of valve pins mounted on a common yoke plate which is actuated by a common actuator. The adjustment device includes a pin holder for holding the valve pin, and a locking member. The pin holder has two threaded portions. The first threaded portion is adjustable within a threaded receiving hole in the yoke plate. The second threaded portion is received by the locking member. The locking member has a bottom shoulder which butts against the yoke plate, and retains the pin holder in place.

Abstract: The invention relates to an adjustment device for a valve pin on an injection molding machine, and particularly on a multi-cavity machine, having a plurality of valve pins mounted on a common yoke plate which is actuated by a common actuator. The adjustment device includes a pin holder for holding the valve pin, and a locking member. The pin holder has two threaded portions. The first threaded portion is adjustable within a threaded receiving hole in the yoke plate. The second threaded portion is received by the locking member. The locking member has a bottom shoulder which butts against the yoke plate, and retains the pin holder in place.