Abstract: An observation chamber for three-dimensional kinematic profiling of subject animals. One example system for analyzing free-moving animal subject behavior includes a housing, a transparent arena configured to hold a subject, a camera mounted below the arena, and a mirror system configured to reflect multiple images of the subject in a single field of view of the camera. A height of the housing is less than 80 inches, and a width of the housing is less than 32 inches. The system fits within standard doorway dimensions.

Abstract: Disclosed herein are protein-RNA display constructs that couple a protein of interest to its encoding mRNA. An example protein-RNA display construct includes a first nucleotide portion including an RNA that forms hairpin structures; a second nucleotide portion including an mRNA encoding a protein of interest; a first protein portion including a protein having RNA hairpin binding peptides that can specifically bind to the RNA hairpin structures; and a second protein portion including the protein of interest. The protein-RNA display constructs take advantage of binding interactions between RNA hairpin structures and RNA hairpin binding peptides to stably couple the protein of interest to its encoding mRNA. Also disclosed are nucleic acids encoding the protein-RNA display constructs, libraries and kits including the protein-RNA display constructs, and methods of using the protein-RNA display constructs in high-throughput display applications.

Abstract: Disclosed herein are compounds that have improved cellular specificity. The compounds have linkers and other moieties that can both aid in cellular specificity and that can attach functional groups to a target cell. The compounds can be used, e.g., in methods of modulating, detecting, and labeling of proteins and cells. An example method includes the compound forming a covalent bond with a dehalogenase variant.

Abstract: Disclosed herein are electrically conductive polymer compositions comprising a polymer and a dispersant, and methods of synthesizing the polymers therein. Also disclosed herein are microelectrode arrays comprising the electrically conductive material and a conductive polymer composition attached to a surface of the electrically conductive material. Further disclosed herein are methods of detecting electrical activity in a cell comprising contacting the microelectrode array with a cell and detecting electrical activity in the cell by covalently attaching the polymer of the conductive polymer composition to a protein on the surface of the cell.

Abstract: An observation chamber for three-dimensional kinematic profiling of subject animals. One example system for analyzing free-moving animal subject behavior includes a housing, a transparent arena configured to hold a subject, a camera mounted below the arena, and a mirror system configured to reflect multiple images of the subject in a single field of view of the camera. A height of the housing is less than 80 inches, and a width of the housing is less than 32 inches. The system fits within standard doorway dimensions.

Abstract: A ferroelectric phase shifting antenna array consists of a plurality of lrally spaced antenna patches which have respective ferroelectric components abutting an edge of each patch at a near central location on the edge to provide impedance matching. DC control power lines are connected to the center voltage null position of the resonant mode of the antenna patch. An Rf source is connected to the other ends of the ferroelectric components through quarter wave coupled lines which provides simultaneous impedance matching and DC isolation.