Patents by Inventor Michael Tarasev

Michael Tarasev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11933780
    Abstract: A method for assessing an effect, such as a treatment, disease, or passage of time on a cellular unit of an organism includes providing a sample including a cellular unit subject to the effect in a supporting medium in a hermetically sealed container. The container is deoxygenated to induce at least one of a predetermined amount of hypoxia and a predetermined rate of oxygen consumption in the medium. Values for variables associated with the medium and/or the cellular unit are determined at each of a plurality of different times. Various combinations of the predetermined amount of hypoxia, the predetermined rate of oxygen consumption, the values for the variables associated with the medium and/or cellular unit, and the times and then correlated to generate a multi-dimensional surface. This surface is compared with another surface correlating corresponding values relating to another cellular unit such as one not subject to the effect.
    Type: Grant
    Filed: August 17, 2022
    Date of Patent: March 19, 2024
    Assignee: Functional Fluidics Inc.
    Inventors: Michael Tarasev, Xiufeng Gao, Marta Ferranti
  • Publication number: 20240060958
    Abstract: A method for assessing an effect, such as a treatment, disease, or passage of time on a cellular unit of an organism includes providing a sample including a cellular unit subject to the effect in a supporting medium in a hermetically sealed container. The container is deoxygenated to induce at least one of a predetermined amount of hypoxia and a predetermined rate of oxygen consumption in the medium. Values for variables associated with the medium and/or the cellular unit are determined at each of a plurality of different times. Various combinations of the predetermined amount of hypoxia, the predetermined rate of oxygen consumption, the values for the variables associated with the medium and/or cellular unit, and the times and then correlated to generate a multi-dimensional surface. This surface is compared with another surface correlating corresponding values relating to another cellular unit such as one not subject to the effect.
    Type: Application
    Filed: August 17, 2022
    Publication date: February 22, 2024
    Inventors: Michael Tarasev, Xiufeng Gao, Marta Ferranti
  • Publication number: 20230258662
    Abstract: A method for assessing an effect of hypoxia on a tissue includes providing a sample of the tissue in a hermetically sealed container, determining a first amount of a reaction substrate (e.g., protocatechuic acid) to be introduced into the sealed container and determining a second amount of a reaction enzyme (e.g., protocatechuate dioxygenase) to be introduced into the sealed container. The method further includes introducing the reaction substrate and the reaction enzyme into the sealed container. At least one of the first amount of the reaction substrate and the second amount of the reaction enzyme is selected to induce at least one of a predetermined amount of hypoxia less than anoxia and a predetermined rate of hypoxia in the tissue during a reaction between the reaction substrate and the reaction enzyme. Values of properties of the tissue can be measured before and after the reaction to assess effects of hypoxia.
    Type: Application
    Filed: April 25, 2023
    Publication date: August 17, 2023
    Inventors: Michael Tarasev, Lucas Gelmini
  • Patent number: 11662353
    Abstract: A method for assessing an effect of hypoxia on a tissue includes providing a sample of the tissue in a hermetically sealed container, determining a first amount of a reaction substrate (e.g., protocatechuic acid) to be introduced into the sealed container and determining a second amount of a reaction enzyme (e.g., protocatechuate dioxygenase) to be introduced into the sealed container. The method further includes introducing the reaction substrate and the reaction enzyme into the sealed container. At least one of the first amount of the reaction substrate and the second amount of the reaction enzyme is selected to induce at least one of a predetermined amount of hypoxia less than anoxia and a predetermined rate of hypoxia in the tissue during a reaction between the reaction substrate and the reaction enzyme. Values of properties of the tissue can be measured before and after the reaction to assess effects of hypoxia.
    Type: Grant
    Filed: September 10, 2020
    Date of Patent: May 30, 2023
    Assignee: Functional Fluidics Inc.
    Inventors: Michael Tarasev, Lucas Gelmini
  • Publication number: 20220074955
    Abstract: A method for assessing an effect of hypoxia on a tissue includes providing a sample of the tissue in a hermetically sealed container, determining a first amount of a reaction substrate(e.g., protocatechuic acid) to be introduced into the sealed container and determining a second amount of a reaction enzyme (e.g., protocatechuate dioxygenase) to be introduced into the sealed container. The method further includes introducing the reaction substrate and the reaction enzyme into the sealed container. At least one of the first amount of the reaction substrate and the second amount of the reaction enzyme is selected to induce at least one of a predetermined amount of hypoxia less than anoxia and a predetermined rate of hypoxia in the tissue during a reaction between the reaction substrate and the reaction enzyme. Values of properties of the tissue can be measured before and after the reaction to assess effects of hypoxia.
    Type: Application
    Filed: September 10, 2020
    Publication date: March 10, 2022
    Inventors: Michael Tarasev, Lucas Gelmini
  • Publication number: 20210231640
    Abstract: A bead mill and an associated bead-mill-based machine for testing mechanical fragility of red blood cells, employing a cartridge configured to contain a sample while cells get stressed via bead oscillation and, in the case of the fragility testing machine, also while lysis levels get detected, for presentation of fragility information.
    Type: Application
    Filed: April 14, 2021
    Publication date: July 29, 2021
    Inventors: Kenneth Alfano, Michael Tarasev, Steven Meines, Terrance Boyd, Aaron Kehrer, Gene Parunak
  • Patent number: 11009500
    Abstract: A bead mill and an associated bead-mill-based machine for testing mechanical fragility of red blood cells, employing a cartridge configured to contain a sample while cells get stressed via bead oscillation and, in the case of the fragility testing machine, also while lysis levels get detected, for presentation of fragility information.
    Type: Grant
    Filed: May 10, 2019
    Date of Patent: May 18, 2021
    Assignee: Blaze Medical Devices, Inc.
    Inventors: Kenneth Alfano, Michael Tarasev, Steven Meines, Terrance Boyd, Aaron Kehrer, Gene Parunak
  • Patent number: 10900954
    Abstract: A method using variable bead-oscillation-based stress parameters, including relative bead dimensionality or other structural features as well as stress duration or intensity or overall magnitude, in inducing different shear stresses in biological samples, for testing the effects of different combinations of various defined stress conditions.
    Type: Grant
    Filed: April 13, 2016
    Date of Patent: January 26, 2021
    Assignee: Blaze Medical Devices, Inc.
    Inventors: Kenneth Alfano, Michael Tarasev, Sumita Chakraborty
  • Patent number: 10495629
    Abstract: A bead mill and an associated bead-mill-based machine for testing mechanical fragility of red blood cells, employing a cartridge configured to contain a sample while cells get stressed via bead oscillation and, in the case of the fragility testing machine, also while lysis levels get detected, for presentation of fragility information.
    Type: Grant
    Filed: January 22, 2014
    Date of Patent: December 3, 2019
    Assignee: Blaze Medical Devices, Inc.
    Inventors: Kenneth Alfano, Michael Tarasev, Steven Meines, Terrance Boyd, Aaron Kehrer, Gene Parunak
  • Publication number: 20190265227
    Abstract: A bead mill and an associated bead-mill-based machine for testing mechanical fragility of red blood cells, employing a cartridge configured to contain a sample while cells get stressed via bead oscillation and, in the case of the fragility testing machine, also while lysis levels get detected, for presentation of fragility information.
    Type: Application
    Filed: May 10, 2019
    Publication date: August 29, 2019
    Inventors: Kenneth Alfano, Michael Tarasev, Steven Meines, Terrance Boyd, Aaron Kehrer, Gene Parunak
  • Publication number: 20160305929
    Abstract: A method using variable bead-oscillation-based stress parameters, including relative bead dimensionality or other structural features as well as stress duration or intensity or overall magnitude, in inducing different shear stresses in biological samples, for testing the effects of different combinations of various defined stress conditions.
    Type: Application
    Filed: April 13, 2016
    Publication date: October 20, 2016
    Inventors: Kenneth Alfano, Michael Tarasev, Sumita Chakraborty
  • Publication number: 20160011171
    Abstract: A bead mill and an associated bead-mill-based machine for testing mechanical fragility of red blood cells, employing a cartridge configured to contain a sample while cells get stressed via bead oscillation and, in the case of the fragility testing machine, also while lysis levels get detected, for presentation of fragility information.
    Type: Application
    Filed: January 22, 2014
    Publication date: January 14, 2016
    Inventors: Kenneth Alfano, Michael Tarasev, Steven Meines, Terrance Boyd, Aaron Kehrer, Gene Parunak
  • Publication number: 20150153321
    Abstract: A method for using red blood cell deformability testing to improve management of blood product comprising RBC, the method comprising: generating deformability data for red blood cells corresponding to a respective unit of blood product; correlating the deformability data with red blood cell viability or efficacy based on available data; obtaining a representation of quality for the respective unit; and based on the representation of quality assigning a rank and/or timing a transfer.
    Type: Application
    Filed: February 11, 2015
    Publication date: June 4, 2015
    Inventors: Michael Tarasev, Kenneth Alfano
  • Publication number: 20140315289
    Abstract: A disposable cartridge for use in red blood cell mechanical fragility profiling, comprising a stressing section with a lysing chamber in which the sample can receive multiple increments of mechanical stress to cause lysis, and a detection section with a transparent region that at least sometimes can have a thickness suitable to take optical readings that can directly or indirectly reflect a fraction/proportion of cells lysed after each increment of the stress, whereby said region can capture at least a representative portion of the sample that received each increment of stress. In some embodiments, the stressing section and the detection section are the same, and/or the transparent region is flexible or otherwise compressible, and/or the stress is ultrasonic. In some embodiments, the sections are separate, and/or the region is rigid or has a fixed thickness, and/or the stress may comprise cell-to-wall interaction and associated fluidic or viscous shear stress induced via concentric/coaxial objects.
    Type: Application
    Filed: June 30, 2014
    Publication date: October 23, 2014
    Inventors: Michael Tarasev, Kenneth Alfano, Sumita Chakraborty
  • Publication number: 20140017718
    Abstract: A test for erythrocyte membrane mechanical fragility, involving subjection of a sample comprising red blood cells to a mechanical stress capable of causing hemolysis, and measurement of the hemolysis present after particular extent(s) of the stress. Measurement of hemolysis can be without component separation, such as by cell-counting (optical or non-optical) or by spectral differentiation between cell-free and intracellular hemoglobin. Such tests can incorporate a disposable single-use component that contains an RBC sample during testing, and for certain embodiments the mechanical stress involved comprises (higher-energy) ultrasonic stress.
    Type: Application
    Filed: September 18, 2013
    Publication date: January 16, 2014
    Applicant: Blaze Medical Devices, LLC
    Inventors: Michael Tarasev, Kenneth Alfano, Sumita Chakraborty
  • Publication number: 20130144247
    Abstract: A storage bag for containing biologic content having a first chamber operable to contain the biologic content, a second chamber separate from the first chamber, and a first fluidic seal system disposed between the first chamber and the second chamber. The first fluidic seal system is positionable between a first position retaining the biologic content in the first chamber and preventing the biologic content from entering the second chamber and a second position permitting at least a portion of the biologic content to flow from the first chamber to the second chamber.
    Type: Application
    Filed: May 25, 2011
    Publication date: June 6, 2013
    Applicants: BLAZE MEDICAL DEVICES, LLC, THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Robertson Davenport, Michael Tarasev, Kenneth Alfano, Tejinder Singh Deo
  • Patent number: 8268244
    Abstract: The present disclosure describes an apparatus for quantifying the quality degradation of individual stored red blood cell (RBC) units, thereby yielding information to improve decisions regarding their respective allocation, patient suitability, and use. The apparatus for quantifying the quality degradation of individual stored (RBC) units comprises: a hemolysis unit; an optical analysis unit; and a computation unit. This apparatus is amenable to clinical implementation as well as indicative of any given unit's relative viability and thus prospective efficacy. This would provide clinicians with actual data on RBC quality when making decisions about which and how many units to use for transfusion of a given patient. Moreover, deploying this testing throughout the supply chain will improve distribution, planning, and inventory control decisions.
    Type: Grant
    Filed: August 5, 2011
    Date of Patent: September 18, 2012
    Assignee: Blaze Medical Devices, LLC
    Inventors: Michael Tarasev, Kenneth Alfano
  • Patent number: 8263408
    Abstract: A method for quantifying the quality degradation of individual stored red blood cell (RBC) units, thereby yielding information to improve decisions regarding their respective allocation, patient suitability, and use. The method comprises: a hemolysis step; an optical analysis step; and a computation step. The method is amenable to clinical implementation as well as indicative of any given unit's relative viability and thus prospective efficacy. This would provide clinicians with actual data on RBC quality when making decisions about which and how many units to use for transfusion of a given patient. Moreover, deploying this testing throughout the supply chain will improve distribution, planning, and inventory control decisions. A vital aspect of this testing method is the accumulation of copious output and other associated data and the mathematical analyses thereof to optimize algorithms by which to characterize each subsequent test output as meaningfully as possible.
    Type: Grant
    Filed: August 5, 2011
    Date of Patent: September 11, 2012
    Assignee: Blaze Medical Devices, LLC
    Inventors: Michael Tarasev, Kenneth Alfano
  • Publication number: 20120178121
    Abstract: An apparatus and method for quantifying RBC fragility via stress-induced hemolysis and subsequent optical and computational analysis. While directed toward applications in blood quality control, the technology could have application in diagnosis. The apparatus comprises: a hemolysis unit; an optical analysis unit; and a computation unit. Similarly, the associated process comprises: a hemolysis step; an optical analysis step; and a computation step.
    Type: Application
    Filed: March 6, 2012
    Publication date: July 12, 2012
    Inventors: Michael Tarasev, Kenneth Alfano
  • Publication number: 20110318773
    Abstract: A method for assessing the quality degradation of an RBC or whole blood unit, the method comprising: 1) using an ektacytometer to generate erythrocyte deformability data for the RBC or whole blood unit; 2) analyzing the erythrocyte deformability data to assess the quality degradation of the RBC or whole blood unit; 3) providing the erythrocyte deformability data to medical personnel; 4) correlating the erythrocyte deformability data with a clinical outcome of a patient that has used the RBC or whole blood unit; and 5) incorporating the erythrocyte deformability data into subsequent assessments of quality degradation of RBC and whole blood units. This method would provide clinicians with actual data on RBC quality when making decisions about which and how many units to use for transfusion of a given patient. Moreover, deploying the approach throughout the supply chain will improve distribution, planning, and inventory control decisions.
    Type: Application
    Filed: June 24, 2010
    Publication date: December 29, 2011
    Inventors: Michael Tarasev, Kenneth Alfano