Patents by Inventor Michael Tarasev
Michael Tarasev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11933780Abstract: A method for assessing an effect, such as a treatment, disease, or passage of time on a cellular unit of an organism includes providing a sample including a cellular unit subject to the effect in a supporting medium in a hermetically sealed container. The container is deoxygenated to induce at least one of a predetermined amount of hypoxia and a predetermined rate of oxygen consumption in the medium. Values for variables associated with the medium and/or the cellular unit are determined at each of a plurality of different times. Various combinations of the predetermined amount of hypoxia, the predetermined rate of oxygen consumption, the values for the variables associated with the medium and/or cellular unit, and the times and then correlated to generate a multi-dimensional surface. This surface is compared with another surface correlating corresponding values relating to another cellular unit such as one not subject to the effect.Type: GrantFiled: August 17, 2022Date of Patent: March 19, 2024Assignee: Functional Fluidics Inc.Inventors: Michael Tarasev, Xiufeng Gao, Marta Ferranti
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Publication number: 20240060958Abstract: A method for assessing an effect, such as a treatment, disease, or passage of time on a cellular unit of an organism includes providing a sample including a cellular unit subject to the effect in a supporting medium in a hermetically sealed container. The container is deoxygenated to induce at least one of a predetermined amount of hypoxia and a predetermined rate of oxygen consumption in the medium. Values for variables associated with the medium and/or the cellular unit are determined at each of a plurality of different times. Various combinations of the predetermined amount of hypoxia, the predetermined rate of oxygen consumption, the values for the variables associated with the medium and/or cellular unit, and the times and then correlated to generate a multi-dimensional surface. This surface is compared with another surface correlating corresponding values relating to another cellular unit such as one not subject to the effect.Type: ApplicationFiled: August 17, 2022Publication date: February 22, 2024Inventors: Michael Tarasev, Xiufeng Gao, Marta Ferranti
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Publication number: 20230258662Abstract: A method for assessing an effect of hypoxia on a tissue includes providing a sample of the tissue in a hermetically sealed container, determining a first amount of a reaction substrate (e.g., protocatechuic acid) to be introduced into the sealed container and determining a second amount of a reaction enzyme (e.g., protocatechuate dioxygenase) to be introduced into the sealed container. The method further includes introducing the reaction substrate and the reaction enzyme into the sealed container. At least one of the first amount of the reaction substrate and the second amount of the reaction enzyme is selected to induce at least one of a predetermined amount of hypoxia less than anoxia and a predetermined rate of hypoxia in the tissue during a reaction between the reaction substrate and the reaction enzyme. Values of properties of the tissue can be measured before and after the reaction to assess effects of hypoxia.Type: ApplicationFiled: April 25, 2023Publication date: August 17, 2023Inventors: Michael Tarasev, Lucas Gelmini
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Patent number: 11662353Abstract: A method for assessing an effect of hypoxia on a tissue includes providing a sample of the tissue in a hermetically sealed container, determining a first amount of a reaction substrate (e.g., protocatechuic acid) to be introduced into the sealed container and determining a second amount of a reaction enzyme (e.g., protocatechuate dioxygenase) to be introduced into the sealed container. The method further includes introducing the reaction substrate and the reaction enzyme into the sealed container. At least one of the first amount of the reaction substrate and the second amount of the reaction enzyme is selected to induce at least one of a predetermined amount of hypoxia less than anoxia and a predetermined rate of hypoxia in the tissue during a reaction between the reaction substrate and the reaction enzyme. Values of properties of the tissue can be measured before and after the reaction to assess effects of hypoxia.Type: GrantFiled: September 10, 2020Date of Patent: May 30, 2023Assignee: Functional Fluidics Inc.Inventors: Michael Tarasev, Lucas Gelmini
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Publication number: 20220074955Abstract: A method for assessing an effect of hypoxia on a tissue includes providing a sample of the tissue in a hermetically sealed container, determining a first amount of a reaction substrate(e.g., protocatechuic acid) to be introduced into the sealed container and determining a second amount of a reaction enzyme (e.g., protocatechuate dioxygenase) to be introduced into the sealed container. The method further includes introducing the reaction substrate and the reaction enzyme into the sealed container. At least one of the first amount of the reaction substrate and the second amount of the reaction enzyme is selected to induce at least one of a predetermined amount of hypoxia less than anoxia and a predetermined rate of hypoxia in the tissue during a reaction between the reaction substrate and the reaction enzyme. Values of properties of the tissue can be measured before and after the reaction to assess effects of hypoxia.Type: ApplicationFiled: September 10, 2020Publication date: March 10, 2022Inventors: Michael Tarasev, Lucas Gelmini
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Publication number: 20210231640Abstract: A bead mill and an associated bead-mill-based machine for testing mechanical fragility of red blood cells, employing a cartridge configured to contain a sample while cells get stressed via bead oscillation and, in the case of the fragility testing machine, also while lysis levels get detected, for presentation of fragility information.Type: ApplicationFiled: April 14, 2021Publication date: July 29, 2021Inventors: Kenneth Alfano, Michael Tarasev, Steven Meines, Terrance Boyd, Aaron Kehrer, Gene Parunak
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Patent number: 11009500Abstract: A bead mill and an associated bead-mill-based machine for testing mechanical fragility of red blood cells, employing a cartridge configured to contain a sample while cells get stressed via bead oscillation and, in the case of the fragility testing machine, also while lysis levels get detected, for presentation of fragility information.Type: GrantFiled: May 10, 2019Date of Patent: May 18, 2021Assignee: Blaze Medical Devices, Inc.Inventors: Kenneth Alfano, Michael Tarasev, Steven Meines, Terrance Boyd, Aaron Kehrer, Gene Parunak
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Patent number: 10900954Abstract: A method using variable bead-oscillation-based stress parameters, including relative bead dimensionality or other structural features as well as stress duration or intensity or overall magnitude, in inducing different shear stresses in biological samples, for testing the effects of different combinations of various defined stress conditions.Type: GrantFiled: April 13, 2016Date of Patent: January 26, 2021Assignee: Blaze Medical Devices, Inc.Inventors: Kenneth Alfano, Michael Tarasev, Sumita Chakraborty
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Patent number: 10495629Abstract: A bead mill and an associated bead-mill-based machine for testing mechanical fragility of red blood cells, employing a cartridge configured to contain a sample while cells get stressed via bead oscillation and, in the case of the fragility testing machine, also while lysis levels get detected, for presentation of fragility information.Type: GrantFiled: January 22, 2014Date of Patent: December 3, 2019Assignee: Blaze Medical Devices, Inc.Inventors: Kenneth Alfano, Michael Tarasev, Steven Meines, Terrance Boyd, Aaron Kehrer, Gene Parunak
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Publication number: 20190265227Abstract: A bead mill and an associated bead-mill-based machine for testing mechanical fragility of red blood cells, employing a cartridge configured to contain a sample while cells get stressed via bead oscillation and, in the case of the fragility testing machine, also while lysis levels get detected, for presentation of fragility information.Type: ApplicationFiled: May 10, 2019Publication date: August 29, 2019Inventors: Kenneth Alfano, Michael Tarasev, Steven Meines, Terrance Boyd, Aaron Kehrer, Gene Parunak
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Publication number: 20160305929Abstract: A method using variable bead-oscillation-based stress parameters, including relative bead dimensionality or other structural features as well as stress duration or intensity or overall magnitude, in inducing different shear stresses in biological samples, for testing the effects of different combinations of various defined stress conditions.Type: ApplicationFiled: April 13, 2016Publication date: October 20, 2016Inventors: Kenneth Alfano, Michael Tarasev, Sumita Chakraborty
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Publication number: 20160011171Abstract: A bead mill and an associated bead-mill-based machine for testing mechanical fragility of red blood cells, employing a cartridge configured to contain a sample while cells get stressed via bead oscillation and, in the case of the fragility testing machine, also while lysis levels get detected, for presentation of fragility information.Type: ApplicationFiled: January 22, 2014Publication date: January 14, 2016Inventors: Kenneth Alfano, Michael Tarasev, Steven Meines, Terrance Boyd, Aaron Kehrer, Gene Parunak
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Publication number: 20150153321Abstract: A method for using red blood cell deformability testing to improve management of blood product comprising RBC, the method comprising: generating deformability data for red blood cells corresponding to a respective unit of blood product; correlating the deformability data with red blood cell viability or efficacy based on available data; obtaining a representation of quality for the respective unit; and based on the representation of quality assigning a rank and/or timing a transfer.Type: ApplicationFiled: February 11, 2015Publication date: June 4, 2015Inventors: Michael Tarasev, Kenneth Alfano
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Publication number: 20140315289Abstract: A disposable cartridge for use in red blood cell mechanical fragility profiling, comprising a stressing section with a lysing chamber in which the sample can receive multiple increments of mechanical stress to cause lysis, and a detection section with a transparent region that at least sometimes can have a thickness suitable to take optical readings that can directly or indirectly reflect a fraction/proportion of cells lysed after each increment of the stress, whereby said region can capture at least a representative portion of the sample that received each increment of stress. In some embodiments, the stressing section and the detection section are the same, and/or the transparent region is flexible or otherwise compressible, and/or the stress is ultrasonic. In some embodiments, the sections are separate, and/or the region is rigid or has a fixed thickness, and/or the stress may comprise cell-to-wall interaction and associated fluidic or viscous shear stress induced via concentric/coaxial objects.Type: ApplicationFiled: June 30, 2014Publication date: October 23, 2014Inventors: Michael Tarasev, Kenneth Alfano, Sumita Chakraborty
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Publication number: 20140017718Abstract: A test for erythrocyte membrane mechanical fragility, involving subjection of a sample comprising red blood cells to a mechanical stress capable of causing hemolysis, and measurement of the hemolysis present after particular extent(s) of the stress. Measurement of hemolysis can be without component separation, such as by cell-counting (optical or non-optical) or by spectral differentiation between cell-free and intracellular hemoglobin. Such tests can incorporate a disposable single-use component that contains an RBC sample during testing, and for certain embodiments the mechanical stress involved comprises (higher-energy) ultrasonic stress.Type: ApplicationFiled: September 18, 2013Publication date: January 16, 2014Applicant: Blaze Medical Devices, LLCInventors: Michael Tarasev, Kenneth Alfano, Sumita Chakraborty
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Publication number: 20130144247Abstract: A storage bag for containing biologic content having a first chamber operable to contain the biologic content, a second chamber separate from the first chamber, and a first fluidic seal system disposed between the first chamber and the second chamber. The first fluidic seal system is positionable between a first position retaining the biologic content in the first chamber and preventing the biologic content from entering the second chamber and a second position permitting at least a portion of the biologic content to flow from the first chamber to the second chamber.Type: ApplicationFiled: May 25, 2011Publication date: June 6, 2013Applicants: BLAZE MEDICAL DEVICES, LLC, THE REGENTS OF THE UNIVERSITY OF MICHIGANInventors: Robertson Davenport, Michael Tarasev, Kenneth Alfano, Tejinder Singh Deo
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Patent number: 8268244Abstract: The present disclosure describes an apparatus for quantifying the quality degradation of individual stored red blood cell (RBC) units, thereby yielding information to improve decisions regarding their respective allocation, patient suitability, and use. The apparatus for quantifying the quality degradation of individual stored (RBC) units comprises: a hemolysis unit; an optical analysis unit; and a computation unit. This apparatus is amenable to clinical implementation as well as indicative of any given unit's relative viability and thus prospective efficacy. This would provide clinicians with actual data on RBC quality when making decisions about which and how many units to use for transfusion of a given patient. Moreover, deploying this testing throughout the supply chain will improve distribution, planning, and inventory control decisions.Type: GrantFiled: August 5, 2011Date of Patent: September 18, 2012Assignee: Blaze Medical Devices, LLCInventors: Michael Tarasev, Kenneth Alfano
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Patent number: 8263408Abstract: A method for quantifying the quality degradation of individual stored red blood cell (RBC) units, thereby yielding information to improve decisions regarding their respective allocation, patient suitability, and use. The method comprises: a hemolysis step; an optical analysis step; and a computation step. The method is amenable to clinical implementation as well as indicative of any given unit's relative viability and thus prospective efficacy. This would provide clinicians with actual data on RBC quality when making decisions about which and how many units to use for transfusion of a given patient. Moreover, deploying this testing throughout the supply chain will improve distribution, planning, and inventory control decisions. A vital aspect of this testing method is the accumulation of copious output and other associated data and the mathematical analyses thereof to optimize algorithms by which to characterize each subsequent test output as meaningfully as possible.Type: GrantFiled: August 5, 2011Date of Patent: September 11, 2012Assignee: Blaze Medical Devices, LLCInventors: Michael Tarasev, Kenneth Alfano
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Publication number: 20120178121Abstract: An apparatus and method for quantifying RBC fragility via stress-induced hemolysis and subsequent optical and computational analysis. While directed toward applications in blood quality control, the technology could have application in diagnosis. The apparatus comprises: a hemolysis unit; an optical analysis unit; and a computation unit. Similarly, the associated process comprises: a hemolysis step; an optical analysis step; and a computation step.Type: ApplicationFiled: March 6, 2012Publication date: July 12, 2012Inventors: Michael Tarasev, Kenneth Alfano
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Publication number: 20110318773Abstract: A method for assessing the quality degradation of an RBC or whole blood unit, the method comprising: 1) using an ektacytometer to generate erythrocyte deformability data for the RBC or whole blood unit; 2) analyzing the erythrocyte deformability data to assess the quality degradation of the RBC or whole blood unit; 3) providing the erythrocyte deformability data to medical personnel; 4) correlating the erythrocyte deformability data with a clinical outcome of a patient that has used the RBC or whole blood unit; and 5) incorporating the erythrocyte deformability data into subsequent assessments of quality degradation of RBC and whole blood units. This method would provide clinicians with actual data on RBC quality when making decisions about which and how many units to use for transfusion of a given patient. Moreover, deploying the approach throughout the supply chain will improve distribution, planning, and inventory control decisions.Type: ApplicationFiled: June 24, 2010Publication date: December 29, 2011Inventors: Michael Tarasev, Kenneth Alfano