Abstract: The invention relates to a calibration process for determining the presence and/or the amount of a target compound in a test sample, using a solid support, attached to the surface of which are a compound capable of binding specifically with said target compound and a calibration probe compound, the molar ratio of said compound Csc to said compound Cs being known, in particular for nucleic acid or protein biochips. The invention also comprises the use of such a support for the self-calibration of a measurement, in particular by surface plasmon resonance or by fluorescence, and also a device or a kit comprising such a support and a standard calibration reagent.