Abstract: A pen sterilization device includes a main body having a first end, a second end and a plurality of opposing sides defining a watertight reservoir for containing a sterilization agent. A pair of watertight valves are positioned along each end of the main body and function to allow ingress and egress of a pen to be cleaned. A partition may be provided within the watertight reservoir having one or more brushes that are suspended in the sterilization agent for scrubbing a pen that is passing through the device.