Abstract: The invention provides an applicator of a spongeous collagen membrane and a kit comprising such applicator and such membrane. The applicator comprises an applicator body having a guide extending in a direction from the proximal end to the distal end of the applicator body and a sliding element slidably comprising a shaft portion movable along the guide. The sliding element is provided at its distal end with a contact portion for contacting the spongeous collagen membrane, and the contact portion of the sliding element comprises mechanical fixation means configured to releasably hold the spongeous collagen membrane without substantially generating forces acting in the distal direction on the spongeous collagen membrane over its area extension. The distal end of the applicator body comprises a release surface configured to apply a release force to the spongeous collagen membrane releasing it from the mechanical fixation means for application to the wound site.